| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 30-Oct-2021 |  Richard Wigglesworth | Leicester Tigers | England | 09-Jun-1983 | 38(144) |
| 30-Oct-2021 |  Jimmy Gopperth | London Wasps | New Zealand | 29-Jun-1983 | 38(124) |
| 30-Oct-2021 |  John Afoa | Bristol Bears | New Zealand | 16-Oct-1983 | 38(15) |
| 06-Nov-2021 |  Agustin Creevy | London Irish | Argentina | 15-Mar-1985 | 36(236) |
| 06-Nov-2021 |  Mike Brown | Newcastle Falcons | England | 04-Sep-1985 | 36(63) |
| 30-Oct-2021 |  Api Ratuniyarawa | Northampton Saints | Fiji | 11-Jul-1986 | 35(112) |
| 30-Oct-2021 | Tom Wood | Northampton Saints | England | 03-Nov-1986 | 34(362) |
| 09-Oct-2021 |  Willi Heinz | Worcester Warriors | England | 24-Nov-1986 | 34(320) |
| 07-Nov-2021 |  Danny Care | Harlequins | England | 02-Jan-1987 | 34(310) |
| 06-Nov-2021 |  Tim Swinson | Saracens | Scotland | 11-Feb-1987 | 34(269) |
| 06-Nov-2021 | David Attwood | Bristol Bears | England | 05-Apr-1987 | 34(216) |
| 19-Sep-2021 | Logoviki Mulipola | Newcastle Falcons | Samoa | 11-Mar-1987 | 34(193) |
| 05-Nov-2021 | Dan Cole | Leicester Tigers | England | 09-May-1987 | 34(181) |
| 23-Oct-2021 |  Kirill Gotovtsev | Gloucester | Russia | 17-Jul-1987 | 34(99) |
| 05-Nov-2021 |  Semesa Rokoduguni | Bath | England | 28-Aug-1987 | 34(70) |
| 06-Nov-2021 | Matthew Garvey | Worcester Warriors | England | 23-Oct-1987 | 34(15) |
| 05-Nov-2021 | Danny Cipriani | Bath | England | 02-Nov-1987 | 34(4) |
| 05-Nov-2021 | Nemani Nadolo | Leicester Tigers | Fiji | 31-Jan-1988 | 33(279) |
| 06-Nov-2021 | Ben Stevenson | Newcastle Falcons | England | 13-Mar-1988 | 33(238) |
| 06-Nov-2021 | Will Chudley | Worcester Warriors | England | 17-Mar-1988 | 33(234) |
| 24-Oct-2021 | Francois Hougaard | London Wasps | South Africa | 06-Apr-1988 | 33(201) |
| 06-Nov-2021 | Alex Goode | Saracens | England | 07-May-1988 | 33(183) |
| 09-Oct-2021 | Juan Pablo Socino | Leicester Tigers | Argentina | 30-May-1988 | 33(132) |
| 06-Nov-2021 | Scott Baldwin | Worcester Warriors | Wales | 12-Jul-1988 | 33(117) |
| 23-Oct-2021 | Joe Simpson | Bath | England | 05-Jul-1988 | 33(110) |
| 24-Sep-2021 | Ed Slater | Gloucester | England | 01-Aug-1988 | 33(54) |
| 31-Oct-2021 | Sean Maitland | Saracens | Scotland | 14-Sep-1988 | 33(47) |
| 06-Nov-2021 | Alapati Leiua | Bristol Bears | Samoa | 21-Sep-1988 | 33(46) |
| 06-Nov-2021 |  Donovan Armand | Exeter Chiefs | England | 23-Sep-1988 | 33(44) |
| 03-Oct-2021 |  William Cliff | Sale Sharks | England | 17-Oct-1988 | 32(351) |
| 29-Oct-2021 | Billy Twelvetrees | Gloucester | England | 15-Nov-1988 | 32(348) |
| 06-Nov-2021 | Nick Phipps | London Irish | Australia | 09-Jan-1989 | 32(301) |
| 29-Oct-2021 | Ben Morgan | Gloucester | England | 18-Feb-1989 | 32(253) |
| 22-Oct-2021 | Courtney Lawes | Northampton Saints | England | 23-Feb-1989 | 32(241) |
| 06-Nov-2021 | Coenraad Oosthuizen | Sale Sharks | South Africa | 22-Mar-1989 | 32(229) |
| 24-Oct-2021 | Tom Cruse | London Wasps | England | 30-Mar-1989 | 32(208) |
| 06-Nov-2021 | Rob Simmons | London Irish | Australia | 19-Apr-1989 | 32(201) |
| 06-Nov-2021 | Byron McGuigan | Sale Sharks | Scotland | 20-Apr-1989 | 32(200) |
| 06-Nov-2021 | Carl Fearns | Newcastle Falcons | England | 25-May-1989 | 32(165) |
| 10-Oct-2021 | Ben Harris | London Wasps | England | 28-Apr-1989 | 32(165) |
| 06-Nov-2021 | Phillip van der Walt | Newcastle Falcons | South Africa | 14-Jul-1989 | 32(115) |
| 23-Oct-2021 | Ben Moon | Exeter Chiefs | England | 14-Jul-1989 | 32(101) |
| 07-Nov-2021 | Matthew Symons | Harlequins | England | 04-Aug-1989 | 32(95) |
| 06-Nov-2021 | Duncan Taylor | Saracens | Scotland | 05-Sep-1989 | 32(62) |
| 23-Oct-2021 | Ben Youngs | Leicester Tigers | England | 05-Sep-1989 | 32(48) |
| 30-Oct-2021 | Graham Kitchener | Worcester Warriors | England | 29-Sep-1989 | 32(31) |
| 30-Oct-2021 | Daniel Biggar | Northampton Saints | Wales | 16-Oct-1989 | 32(14) |
| 06-Nov-2021 | Max Lahiff | Bristol Bears | England | 09-Dec-1989 | 31(332) |
| 30-Oct-2021 | Steve Mafi | London Irish | Tonga | 09-Dec-1989 | 31(326) |
| 06-Nov-2021 | Jack Yeandle | Exeter Chiefs | England | 22-Dec-1989 | 31(320) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).