| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 17-Nov-2023 |  Nathan Michelow | Ampthill | England | 16-May-2004 | 19(184) |
| 17-Nov-2023 |  Zach Carr | London Scottish | England | 03-May-2004 | 19(197) |
| 21-Oct-2023 | Charlie Bracken | Ampthill | England | 09-Dec-2003 | 19(316) |
| 21-Oct-2023 | Sam Worsley | Hartpury College | England | 13-Oct-2003 | 20(8) |
| 28-Oct-2023 | Tobias Elliott | Ampthill | England | 16-Sep-2003 | 20(42) |
| 21-Oct-2023 |  Georgie Irvine | Cambridge | England | 05-Aug-2003 | 20(77) |
| 20-Oct-2023 |  Iwan Jenkins | Cornish Pirates | England | 13-Mar-2003 | 20(221) |
| 21-Oct-2023 | Kaden Pearce-Paul | Ampthill | England | 13-Mar-2003 | 20(222) |
| 17-Nov-2023 | Cassius Cleaves | London Scottish | England | 15-Mar-2003 | 20(247) |
| 04-Nov-2023 |  Obinna Nkwocha | Coventry | England | 20-Dec-2002 | 20(319) |
| 21-Oct-2023 | Francis Moore | Ampthill | England | 12-Nov-2002 | 20(343) |
| 28-Oct-2023 | Jevaughn Warren | Ampthill | England | 18-Jul-2002 | 21(102) |
| 04-Nov-2023 | Ollie Hartley | Ampthill | England | 19-Feb-2002 | 21(258) |
| 21-Oct-2023 | Joe Peard | Ampthill | Wales | 02-Feb-2002 | 21(261) |
| 04-Nov-2023 | Samson Adejimi | Ampthill | England | 15-Feb-2002 | 21(262) |
| 20-Oct-2023 | Dan John | Cornish Pirates | Wales | 04-Oct-2001 | 22(16) |
| 04-Nov-2023 | Louis James | Coventry | England | 29-Sep-2001 | 22(36) |
| 17-Nov-2023 | Brandon Jackson | Ampthill | England | 21-Sep-2001 | 22(57) |
| 20-Oct-2023 | Ioan Evans | Cornish Pirates | Wales | 18-Jul-2001 | 22(94) |
| 04-Nov-2023 | Luke Green | Ampthill | England | 06-Jun-2001 | 22(151) |
| 21-Oct-2023 | Harvey Beaton | Ampthill | England | 15-Mar-2001 | 22(220) |
| 04-Nov-2023 | Oliver Stonham | Ampthill | England | 16-Feb-2001 | 22(261) |
| 28-Oct-2023 |  Come Joussain | Nottingham | France | 18-Jan-2001 | 22(283) |
| 20-Oct-2023 | Jonathan Law | London Scottish | England | 19-Dec-2000 | 22(305) |
| 21-Oct-2023 | Tom Bacon | Ampthill | England | 03-Nov-2000 | 22(352) |
| 21-Oct-2023 | Joshua Smart | Ampthill | England | 15-Sep-2000 | 23(35) |
| 20-Oct-2023 | Robbie McCallum | London Scottish | Scotland | 11-Jun-2000 | 23(130) |
| 20-Oct-2023 | Ioan Rhys Davis | London Scottish | Wales | 12-Apr-2000 | 23(190) |
| 20-Oct-2023 | Matas Jurevicius | London Scottish | England | 07-Apr-2000 | 23(195) |
| 21-Oct-2023 | Alex Morgan | Hartpury College | England | 26-Nov-1999 | 23(329) |
| 21-Oct-2023 | Iestyn Rees | Ampthill | Wales | 05-Oct-1999 | 24(16) |
| 20-Oct-2023 | Luke Mehson | London Scottish | England | 18-Sep-1999 | 24(32) |
| 20-Oct-2023 | Cameron Anderson | London Scottish | Scotland | 16-Sep-1999 | 24(34) |
| 21-Oct-2023 | Ben Harris | Ampthill | England | 08-Sep-1999 | 24(43) |
| 21-Oct-2023 | Oliver Morris | Ampthill | England | 19-Aug-1999 | 24(63) |
| 17-Nov-2023 | Kapeliele Pifeletijr | Ampthill | United States | 01-Sep-1999 | 24(77) |
| 20-Oct-2023 | George Head | London Scottish | England | 04-Aug-1999 | 24(77) |
| 21-Oct-2023 |  Oliver Fox | Doncaster Knights | England | 14-Mar-1999 | 24(221) |
| 28-Oct-2023 | Kai Owen | Nottingham | England | 27-Feb-1999 | 24(243) |
| 04-Nov-2023 | Arthur Cordwell | Coventry | England | 03-Mar-1999 | 24(246) |
| 21-Oct-2023 | Harry Bazalgette | Hartpury College | England | 27-Jan-1999 | 24(267) |
| 21-Oct-2023 | Harry Davey | Doncaster Knights | England | 21-Dec-1998 | 24(304) |
| 21-Oct-2023 | Fraser Strachan | Ampthill | England | 18-Sep-1998 | 25(33) |
| 28-Oct-2023 | Xavier Valentine | Nottingham | England | 22-Jul-1998 | 25(98) |
| 20-Oct-2023 | Matthew McNab | Cornish Pirates | Zimbabwe | 08-Jun-1998 | 25(134) |
| 21-Oct-2023 | Matt Williams | Cambridge | England | 16-Mar-1998 | 25(219) |
| 20-Oct-2023 | Matthew Johnson | Cornish Pirates | England | 21-Nov-1997 | 25(333) |
| 17-Nov-2023 | Jack Ingall | London Scottish | England | 19-Dec-1997 | 25(333) |
| 04-Nov-2023 | Suva Ma'asi | Coventry | England | 08-Nov-1997 | 25(361) |
| 20-Oct-2023 | Will Talbot-Davies | London Scottish | Wales | 11-Sep-1997 | 26(39) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).