| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 19-Jun-2021 |  Louis Lynagh | Harlequins | Italy | 03-Dec-2000 | 20(198) |
| 19-Jun-2021 |  Richard Capstick | Exeter Chiefs | England | 13-Feb-2000 | 21(127) |
| 19-Jun-2021 | Jack Kenningham | Harlequins | England | 19-Nov-1999 | 21(213) |
| 26-Jun-2021 | Cadan Murley | Harlequins | England | 31-Jul-1999 | 21(331) |
| 19-Jun-2021 |  Arron Reed | Sale Sharks | England | 10-Jul-1999 | 21(345) |
| 19-Jun-2021 | Marcus Smith | Harlequins | England | 14-Feb-1999 | 22(126) |
| 19-Jun-2021 | Tom Curry | Sale Sharks | England | 15-Jun-1998 | 23(4) |
| 19-Jun-2021 | Ben Curry | Sale Sharks | England | 15-Jun-1998 | 23(4) |
| 19-Jun-2021 | Tyrone Green | Harlequins | South Africa | 05-Mar-1998 | 23(106) |
| 19-Jun-2021 |  Benjamin Earl | Bristol Bears | England | 07-Jan-1998 | 23(163) |
| 19-Jun-2021 | Curtis Langdon | Sale Sharks | England | 03-Aug-1997 | 23(320) |
| 19-Jun-2021 | Alex Dombrandt | Harlequins | England | 15-May-1997 | 24(35) |
| 19-Jun-2021 | Jack Maunder | Exeter Chiefs | England | 05-Apr-1997 | 24(75) |
| 19-Jun-2021 | Max Malins | Bristol Bears | England | 07-Jan-1997 | 24(163) |
| 19-Jun-2021 | Cobus Wiese | Sale Sharks | South Africa | 02-Jan-1997 | 24(168) |
| 19-Jun-2021 | Joe Simmonds | Exeter Chiefs | England | 19-Dec-1996 | 24(182) |
| 19-Jun-2021 | Joe Marchant | Harlequins | England | 16-Jul-1996 | 24(338) |
| 19-Jun-2021 | Andy Uren | Bristol Bears | England | 20-Feb-1996 | 25(120) |
| 19-Jun-2021 | Callum Sheedy | Bristol Bears | Wales | 28-Oct-1995 | 25(235) |
| 19-Jun-2021 | Daniel du Preez | Sale Sharks | South Africa | 05-Aug-1995 | 25(319) |
| 19-Jun-2021 | Jean Luc du Preez | Sale Sharks | South Africa | 05-Aug-1995 | 25(319) |
| 26-Jun-2021 | James Chisholm | Harlequins | England | 11-Aug-1995 | 25(320) |
| 19-Jun-2021 | Aaron Morris | Harlequins | England | 10-Jan-1995 | 26(161) |
| 19-Jun-2021 | Sam Simmonds | Exeter Chiefs | England | 10-Nov-1994 | 26(222) |
| 19-Jun-2021 | Rohan Janse van Rensburg | Sale Sharks | South Africa | 11-Sep-1994 | 26(282) |
| 19-Jun-2021 | Thomas O'Flaherty | Exeter Chiefs | England | 21-Jul-1994 | 26(334) |
| 19-Jun-2021 | Wilco Louw | Harlequins | South Africa | 20-Jul-1994 | 26(335) |
| 19-Jun-2021 | Jonny Hill | Exeter Chiefs | England | 08-Jun-1994 | 27(11) |
| 26-Jun-2021 | Andre Esterhuizen | Harlequins | South Africa | 30-Mar-1994 | 27(88) |
| 19-Jun-2021 | Jonny Gray | Exeter Chiefs | Scotland | 14-Mar-1994 | 27(97) |
| 19-Jun-2021 | Harry Thacker | Bristol Bears | England | 18-Feb-1994 | 27(121) |
| 19-Jun-2021 | Johannes Kirsten | Exeter Chiefs | South Africa | 01-Dec-1993 | 27(200) |
| 19-Jun-2021 | Thomas Lawday | Harlequins | England | 11-Nov-1993 | 27(220) |
| 19-Jun-2021 | Ollie Devoto | Exeter Chiefs | England | 22-Sep-1993 | 27(270) |
| 19-Jun-2021 | Robert du Preez | Sale Sharks | South Africa | 30-Jul-1993 | 27(324) |
| 19-Jun-2021 | Luke Cowan-Dickie | Exeter Chiefs | England | 20-Jun-1993 | 27(364) |
| 19-Jun-2021 | Jack Nowell | Exeter Chiefs | England | 11-Apr-1993 | 28(69) |
| 19-Jun-2021 | Alec Hepburn | Exeter Chiefs | England | 30-Mar-1993 | 28(81) |
| 19-Jun-2021 | Kyle Sinckler | Bristol Bears | England | 30-Mar-1993 | 28(81) |
| 19-Jun-2021 | Simon Hammersley | Sale Sharks | England | 20-Mar-1993 | 28(91) |
| 19-Jun-2021 | Henry Slade | Exeter Chiefs | England | 19-Mar-1993 | 28(92) |
| 19-Jun-2021 | Christopher Vui | Bristol Bears | Samoa | 11-Feb-1993 | 28(128) |
| 19-Jun-2021 | Ross Harrison | Sale Sharks | England | 03-Sep-1992 | 28(289) |
| 19-Jun-2021 | Semi Radradra | Bristol Bears | Fiji | 13-Jun-1992 | 29(6) |
| 19-Jun-2021 | Stephan Lewies | Harlequins | South Africa | 27-Jan-1992 | 29(144) |
| 19-Jun-2021 | Charles Piutau | Bristol Bears | New Zealand | 31-Oct-1991 | 29(232) |
| 19-Jun-2021 | Faf de Klerk | Sale Sharks | South Africa | 19-Oct-1991 | 29(244) |
| 19-Jun-2021 | Harry Williams | Exeter Chiefs | England | 01-Oct-1991 | 29(262) |
| 19-Jun-2021 | Nathan Hughes | Bristol Bears | England | 10-Jun-1991 | 30(10) |
| 19-Jun-2021 | Manu Tuilagi | Sale Sharks | England | 18-May-1991 | 30(33) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).