| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 13-Nov-1999 |  Andrew Higgins | Worcester Warriors | England | 13-Jul-1981 | 18(123) |
| 13-Nov-1999 |  Ross Winney | Wakefield | England | 25-May-1981 | 18(172) |
| 29-Jan-2000 |  Ben Gollings | Harlequins | England | 12-May-1980 | 19(261) |
| 03-Jan-2000 | Matthew Williams | Harlequins | England | 08-Feb-1980 | 19(329) |
| 03-Jan-2000 |  David Flatman | Saracens | England | 21-Jan-1980 | 19(347) |
| 02-Jan-2000 |  Sean Marsden | Bristol Bears | England | 17-Jan-1980 | 19(350) |
| 03-Jan-2000 | Paul Sackey | Bedford Blues | England | 08-Nov-1979 | 20(56) |
| 03-Jan-2000 |  Hall Charlton | Newcastle Falcons | England | 25-Oct-1979 | 20(70) |
| 03-Jan-2000 | Steve Borthwick | Bath | England | 12-Oct-1979 | 20(83) |
| 03-Jan-2000 |  Alex Sanderson | Sale Sharks | England | 07-Oct-1979 | 20(88) |
| 02-Jan-2000 |  Mark Gilbert | Coventry | England | 24-Sep-1979 | 20(100) |
| 16-Oct-1999 |  Jon Skurr | Otley | England | 08-Jul-1979 | 20(100) |
| 03-Jan-2000 |  Lee Wakelam | Orrell | England | 18-Sep-1979 | 20(107) |
| 14-Nov-1999 |  Andy Hall | Moseley | Scotland | 05-Jun-1979 | 20(162) |
| 13-Nov-1999 | Chris Bentley | Orrell | England | 24-May-1979 | 20(173) |
| 03-Jan-2000 |  Kieran Campbell | London Irish | Ireland | 06-Jul-1979 | 20(181) |
| 02-Jan-2000 | Gareth Bowen | Bristol Bears | Wales | 20-Jun-1979 | 20(196) |
| 13-Nov-1999 | Simon Amor | Coventry | England | 25-Apr-1979 | 20(202) |
| 03-Jan-2000 | Steve Hanley | Sale Sharks | England | 11-Jun-1979 | 20(206) |
| 03-Jan-2000 | Hefin O'Hare | Leeds Tykes | Scotland | 02-Jun-1979 | 20(215) |
| 03-Jan-2000 | Jonny Wilkinson | Newcastle Falcons | England | 25-May-1979 | 20(223) |
| 13-Nov-1999 | Tom Palmer | Leeds Tykes | England | 27-Mar-1979 | 20(231) |
| 03-Jan-2000 | Jamie Noon | Newcastle Falcons | England | 09-May-1979 | 20(239) |
| 13-Nov-1999 |  Dan Smaje | Henley Hawks | England | 28-Feb-1979 | 20(258) |
| 03-Jan-2000 | Iain Balshaw | Bath | England | 18-Apr-1979 | 20(260) |
| 16-Oct-1999 |  Peter Glackin | Birmingham Solihull | England | 26-Jan-1979 | 20(263) |
| 08-Apr-2000 | Alex Brown | Bristol Bears | England | 17-May-1979 | 20(327) |
| 29-Jan-2000 | Rob Thirlby | Saracens | England | 02-Mar-1979 | 20(333) |
| 13-Nov-1999 | Owen Edwards | Wakefield | England | 14-Dec-1978 | 20(334) |
| 30-Jan-2000 | Tom May | Newcastle Falcons | England | 05-Feb-1979 | 20(359) |
| 03-Jan-2000 | Micky Ward | Newcastle Falcons | England | 09-Jan-1979 | 20(359) |
| 03-Jan-2000 | Shaun Berne | Bath | Australia | 08-Jan-1979 | 20(360) |
| 29-Jan-2000 |  Adam Eustace | Gloucester | England | 09-Jan-1979 | 21(20) |
| 16-Oct-1999 | Robert Whatmuff | Otley | England | 12-Sep-1978 | 21(34) |
| 03-Jan-2000 | Ben Johnston | Saracens | England | 08-Nov-1978 | 21(56) |
| 13-Nov-1999 | David Sleman | Orrell | England | 12-Sep-1978 | 21(62) |
| 02-Jan-2000 | Scott Morgan | Bristol Bears | Wales | 17-Oct-1978 | 21(77) |
| 03-Jan-2000 | Mike Tindall | Bath | England | 18-Oct-1978 | 21(77) |
| 16-Oct-1999 |  Sam Allen | Wharfedale | England | 23-Jul-1978 | 21(85) |
| 14-Nov-1999 | Peter Buxton | Moseley | England | 21-Aug-1978 | 21(85) |
| 03-Jan-2000 | Richard Nias | Harlequins | England | 29-Sep-1978 | 21(96) |
| 29-Jan-2000 | Lee Best | Bristol Bears | England | 16-Oct-1978 | 21(105) |
| 29-Jan-2000 | Richard Jewell | Gloucester | England | 11-Oct-1978 | 21(110) |
| 13-Nov-1999 | Chris Simpson-Daniel | Wakefield | England | 15-Jul-1978 | 21(121) |
| 03-Jan-2000 | Martin Shaw | Newcastle Falcons | England | 02-Sep-1978 | 21(123) |
| 29-Jan-2000 |  Ben Cohen | Northampton Saints | England | 14-Sep-1978 | 21(137) |
| 13-Nov-1999 |  Richard Baxter | Exeter Chiefs | England | 23-Jun-1978 | 21(143) |
| 13-Nov-1999 | Paul Newton | Orrell | England | 29-Apr-1978 | 21(198) |
| 02-Jan-2000 | Jim Bramhall | Northampton Saints | England | 20-May-1978 | 21(227) |
| 03-Jan-2000 | Matt Powell | Saracens | Wales | 08-May-1978 | 21(240) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).