| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 13-Nov-1999 |  Guy Parker | Manchester | England | 28-Jan-1963 | 36(289) |
| 03-Jan-2000 |  Dave Baldwin | Sale Sharks | England | 03-Sep-1964 | 35(122) |
| 03-Jan-2000 |  Victor Ubogu | Bath | England | 08-Sep-1964 | 35(117) |
| 13-May-2000 |  Allan Bateman | Northampton Saints | Wales | 06-Mar-1965 | 35(69) |
| 26-Feb-2000 |  Zinzan Brooke | Harlequins | New Zealand | 14-Feb-1965 | 35(12) |
| 08-Apr-2000 |  Kevin Putt | London Irish | Ireland | 28-Jul-1965 | 34(255) |
| 29-Jan-2000 |  Andrew Deacon | Gloucester | England | 31-Jul-1965 | 34(182) |
| 08-Apr-2000 |  Henry Honiball | Bristol Bears | South Africa | 01-Dec-1965 | 34(129) |
| 13-Nov-1999 |  David Scully | Rotherham Titans | England | 07-Aug-1965 | 34(98) |
| 16-Oct-1999 |  Charlie Vyvyan | Wharfedale | England | 01-Sep-1965 | 34(45) |
| 30-Jan-2000 |  George Graham | Newcastle Falcons | Scotland | 19-Jan-1966 | 34(11) |
| 26-Feb-2000 |  Mike White | London Welsh | England | 30-Mar-1966 | 33(333) |
| 26-Feb-2000 | Steve Bachop | London Irish | Samoa | 02-Apr-1966 | 33(330) |
| 29-Jan-2000 | Jim Mallinder | Sale Sharks | England | 16-Mar-1966 | 33(319) |
| 29-Jan-2000 |  Darren Garforth | Leicester Tigers | England | 09-Apr-1966 | 33(295) |
| 08-Apr-2000 | Dean Ryan | Bristol Bears | England | 22-Jun-1966 | 33(291) |
| 13-Nov-1999 | Alan Buzza | Rotherham Titans | England | 03-Mar-1966 | 33(255) |
| 13-May-2000 | Garry Pagel | Northampton Saints | South Africa | 17-Sep-1966 | 33(239) |
| 02-Jan-2000 | Alan Charron | Bristol Bears | Canada | 27-Jul-1966 | 33(159) |
| 29-Jan-2000 | Dawie Theron | Sale Sharks | South Africa | 15-Sep-1966 | 33(136) |
| 30-Jan-2000 | Gary Armstrong | Newcastle Falcons | Scotland | 30-Sep-1966 | 33(122) |
| 26-Feb-2000 | Chris Sheasby | Harlequins | England | 30-Nov-1966 | 33(88) |
| 02-Jan-2000 |  Phil Hopley | Bracknell | England | 06-Oct-1966 | 33(88) |
| 29-Jan-2000 |  Francois Pienaar | Saracens | South Africa | 02-Jan-1967 | 33(27) |
| 29-Jan-2000 | Thierry Lacroix | Saracens | France | 02-Mar-1967 | 32(333) |
| 29-Jan-2000 | Adrian Powles | Gloucester | England | 14-Mar-1967 | 32(321) |
| 26-Feb-2000 | Ian Jones | Gloucester | New Zealand | 17-Apr-1967 | 32(315) |
| 26-Feb-2000 | Paul Graham | Harlequins | Wales | 28-Apr-1967 | 32(304) |
| 26-Feb-2000 | John Schuster | Harlequins | Other | 30-Jun-1967 | 32(241) |
| 26-Feb-2000 | Gareth Rees | Harlequins | Canada | 30-Jun-1967 | 32(241) |
| 29-Jan-2000 |  Mark Fountaine | Coventry | England | 07-Jun-1967 | 32(236) |
| 02-Jan-2000 | Guy Spencer | Bracknell | England | 22-Jun-1967 | 32(194) |
| 13-Nov-1999 | Terry Garnett | Rotherham Titans | England | 10-May-1967 | 32(187) |
| 29-Jan-2000 | Dorian West | Leicester Tigers | England | 05-Oct-1967 | 32(116) |
| 26-Feb-2000 | Ian Buckett | London Welsh | Wales | 23-Dec-1967 | 32(65) |
| 03-Jan-2000 | Jon Preston | Bath | New Zealand | 15-Nov-1967 | 32(49) |
| 29-Jan-2000 | Kevin Nepia | Harlequins | New Zealand | 27-Dec-1967 | 32(33) |
| 26-Feb-2000 | Gregg Botterman | London Welsh | England | 03-Mar-1968 | 31(360) |
| 02-Jan-2000 |  Andy Parton | Henley Hawks | England | 31-Jan-1968 | 31(336) |
| 26-Feb-2000 | Richard Tombs | Gloucester | Australia | 01-Apr-1968 | 31(331) |
| 03-Jan-2000 | Chris Wright | Harlequins | England | 08-Feb-1968 | 31(329) |
| 03-Jan-2000 | Tim Fourie | Leeds Tykes | England | 12-Mar-1968 | 31(297) |
| 29-Jan-2000 | Dave Lougheed | Leicester Tigers | Canada | 11-Apr-1968 | 31(292) |
| 03-Jan-2000 | Eddie Jones | Leeds Tykes | England | 22-Mar-1968 | 31(286) |
| 13-Nov-1999 | Mark McAtamney | London Welsh | New Zealand | 19-Feb-1968 | 31(268) |
| 03-Jan-2000 | Ben Clarke | Bath | England | 15-Apr-1968 | 31(262) |
| 26-Feb-2000 | Ian Dunston | London Welsh | England | 11-Jun-1968 | 31(260) |
| 27-Feb-2000 |  Mark Weedon | London Wasps | New Zealand | 13-Jul-1968 | 31(228) |
| 13-May-2000 | Pat Lam | Northampton Saints | Samoa | 29-Sep-1968 | 31(226) |
| 08-Apr-2000 | Jarrod Cunningham | London Irish | * | 07-Sep-1968 | 31(213) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).