| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 21-May-2016 |  Maro Itoje | Saracens | England | 28-Oct-1994 | 21(206) |
| 21-May-2016 |  Harry Thacker | Leicester Tigers | England | 18-Feb-1994 | 22(93) |
| 21-May-2016 |  Luke Cowan-Dickie | Exeter Chiefs | England | 20-Jun-1993 | 22(336) |
| 21-May-2016 | Jack Nowell | Exeter Chiefs | England | 11-Apr-1993 | 23(40) |
| 21-May-2016 | Dominic Barrow | Leicester Tigers | England | 19-Mar-1993 | 23(63) |
| 21-May-2016 | Henry Slade | Exeter Chiefs | England | 19-Mar-1993 | 23(63) |
| 21-May-2016 | Billy Vunipola | Saracens | England | 03-Nov-1992 | 23(199) |
| 21-May-2016 |  Elliot Daly | London Wasps | England | 08-Oct-1992 | 23(225) |
| 21-May-2016 | Dan Robson | London Wasps | England | 14-Mar-1992 | 24(68) |
| 21-May-2016 | Owen Williams | Leicester Tigers | Wales | 27-Feb-1992 | 24(84) |
| 21-May-2016 | Olly Woodburn | Exeter Chiefs | England | 18-Nov-1991 | 24(185) |
| 21-May-2016 | Charles Piutau | London Wasps | New Zealand | 31-Oct-1991 | 24(203) |
| 21-May-2016 | Harry Williams | Exeter Chiefs | England | 01-Oct-1991 | 24(233) |
| 21-May-2016 | Owen Farrell | Saracens | England | 21-Sep-1991 | 24(243) |
| 21-May-2016 | Nathan Hughes | London Wasps | England | 10-Jun-1991 | 24(346) |
| 21-May-2016 | Manu Tuilagi | Leicester Tigers | England | 18-May-1991 | 25(4) |
| 21-May-2016 | Christian Wade | London Wasps | England | 15-May-1991 | 25(7) |
| 21-May-2016 | Joe Launchberry | London Wasps | England | 12-Apr-1991 | 25(40) |
| 21-May-2016 | Makovina Vunipola | Saracens | England | 13-Jan-1991 | 25(129) |
| 21-May-2016 | Telusa Veainu | Leicester Tigers | Tonga | 26-Dec-1990 | 25(147) |
| 21-May-2016 | Dave Ewers | Exeter Chiefs | England | 03-Nov-1990 | 25(200) |
| 21-May-2016 | Locky McCaffrey | Leicester Tigers | Australia | 19-Mar-1990 | 26(64) |
| 21-May-2016 | George Kruis | Saracens | England | 22-Feb-1990 | 26(89) |
| 21-May-2016 | Jake Cooper-Woolley | London Wasps | England | 18-Nov-1989 | 26(185) |
| 21-May-2016 | Will Fraser | Saracens | England | 29-Oct-1989 | 26(205) |
| 21-May-2016 | Graham Kitchener | Leicester Tigers | England | 29-Sep-1989 | 26(235) |
| 21-May-2016 | Ben Youngs | Leicester Tigers | England | 05-Sep-1989 | 26(259) |
| 21-May-2016 | Duncan Taylor | Saracens | Scotland | 05-Sep-1989 | 26(259) |
| 21-May-2016 | Ben Moon | Exeter Chiefs | England | 14-Jul-1989 | 26(312) |
| 21-May-2016 | Peter Betham | Leicester Tigers | Australia | 06-Jan-1989 | 27(135) |
| 21-May-2016 | Mitchell Lees | Exeter Chiefs | Australia | 12-Oct-1988 | 27(221) |
| 21-May-2016 | Donovan Armand | Exeter Chiefs | England | 23-Sep-1988 | 27(240) |
| 21-May-2016 | Alex Goode | Saracens | England | 07-May-1988 | 28(14) |
| 21-May-2016 | Will Chudley | Exeter Chiefs | England | 17-Mar-1988 | 28(65) |
| 21-May-2016 | Frank Halai | London Wasps | New Zealand | 06-Mar-1988 | 28(76) |
| 21-May-2016 | Michael Rhodes | Saracens | South Africa | 19-Dec-1987 | 28(154) |
| 21-May-2016 | Ian Whitten | Exeter Chiefs | Ireland | 05-Jun-1987 | 28(351) |
| 21-May-2016 | Dan Cole | Leicester Tigers | England | 09-May-1987 | 29(13) |
| 21-May-2016 | Chris Ashton | Saracens | England | 29-Mar-1987 | 29(54) |
| 21-May-2016 | Michael Fitzgerald | Leicester Tigers | New Zealand | 08-Feb-1987 | 29(103) |
| 21-May-2016 | Bradley Barritt | Saracens | England | 07-Aug-1986 | 29(288) |
| 21-May-2016 | Matt Mullan | London Wasps | England | 15-Mar-1986 | 30(68) |
| 21-May-2016 | Matthew Tait | Leicester Tigers | England | 06-Feb-1986 | 30(105) |
| 21-May-2016 | Julian Salvi | Exeter Chiefs | Australia | 09-Nov-1985 | 30(194) |
| 21-May-2016 | Siale Piutau | London Wasps | Tonga | 13-Oct-1985 | 30(221) |
| 21-May-2016 | Phil Dollman | Exeter Chiefs | Wales | 15-May-1985 | 31(7) |
| 21-May-2016 | James Haskell | London Wasps | England | 02-Apr-1985 | 31(49) |
| 21-May-2016 | Bradley Davies | London Wasps | Wales | 09-Jan-1985 | 31(133) |
| 21-May-2016 | Gareth Steenson | Exeter Chiefs | Ireland | 05-Apr-1984 | 32(46) |
| 21-May-2016 | Vereniki Goneva | Leicester Tigers | Fiji | 05-Apr-1984 | 32(46) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).