| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 21-May-2016 |  Carlo Festuccia | London Wasps | Italy | 20-Jun-1980 | 35(336) |
| 21-May-2016 | George Smith | London Wasps | Australia | 17-Jul-1980 | 35(309) |
| 28-May-2016 |  Schalk Brits | Saracens | South Africa | 16-May-1981 | 35(13) |
| 28-May-2016 | Petrus Du Plessis | Saracens | England | 31-May-1981 | 34(363) |
| 21-May-2016 |  Marcos Ayerza | Leicester Tigers | Argentina | 12-Jan-1983 | 33(130) |
| 21-May-2016 | Brendan O'Connor | Leicester Tigers | New Zealand | 31-May-1983 | 32(356) |
| 28-May-2016 | Richard Wigglesworth | Saracens | England | 09-Jun-1983 | 32(354) |
| 21-May-2016 | Jimmy Gopperth | London Wasps | New Zealand | 29-Jun-1983 | 32(327) |
| 28-May-2016 | Chris Wyles | Saracens | United States | 13-Sep-1983 | 32(258) |
| 28-May-2016 |  Geoffrey Parling | Exeter Chiefs | England | 28-Oct-1983 | 32(213) |
| 28-May-2016 | Gareth Steenson | Exeter Chiefs | Ireland | 05-Apr-1984 | 32(53) |
| 21-May-2016 | Vereniki Goneva | Leicester Tigers | Fiji | 05-Apr-1984 | 32(46) |
| 21-May-2016 | Bradley Davies | London Wasps | Wales | 09-Jan-1985 | 31(133) |
| 21-May-2016 | James Haskell | London Wasps | England | 02-Apr-1985 | 31(49) |
| 28-May-2016 | Phil Dollman | Exeter Chiefs | Wales | 15-May-1985 | 31(14) |
| 21-May-2016 | Siale Piutau | London Wasps | Tonga | 13-Oct-1985 | 30(221) |
| 28-May-2016 | Julian Salvi | Exeter Chiefs | Australia | 09-Nov-1985 | 30(201) |
| 21-May-2016 | Matthew Tait | Leicester Tigers | England | 06-Feb-1986 | 30(105) |
| 21-May-2016 | Matt Mullan | London Wasps | England | 15-Mar-1986 | 30(68) |
| 28-May-2016 | Bradley Barritt | Saracens | England | 07-Aug-1986 | 29(295) |
| 21-May-2016 | Michael Fitzgerald | Leicester Tigers | New Zealand | 08-Feb-1987 | 29(103) |
| 28-May-2016 | Chris Ashton | Saracens | England | 29-Mar-1987 | 29(61) |
| 21-May-2016 | Dan Cole | Leicester Tigers | England | 09-May-1987 | 29(13) |
| 28-May-2016 | Ian Whitten | Exeter Chiefs | Ireland | 05-Jun-1987 | 28(358) |
| 28-May-2016 | Michael Rhodes | Saracens | South Africa | 19-Dec-1987 | 28(161) |
| 21-May-2016 | Frank Halai | London Wasps | New Zealand | 06-Mar-1988 | 28(76) |
| 28-May-2016 | Will Chudley | Exeter Chiefs | England | 17-Mar-1988 | 28(72) |
| 28-May-2016 | Alex Goode | Saracens | England | 07-May-1988 | 28(21) |
| 28-May-2016 | Donovan Armand | Exeter Chiefs | England | 23-Sep-1988 | 27(247) |
| 28-May-2016 | Mitchell Lees | Exeter Chiefs | Australia | 12-Oct-1988 | 27(228) |
| 21-May-2016 | Peter Betham | Leicester Tigers | Australia | 06-Jan-1989 | 27(135) |
| 28-May-2016 | Ben Moon | Exeter Chiefs | England | 14-Jul-1989 | 26(319) |
| 28-May-2016 | Duncan Taylor | Saracens | Scotland | 05-Sep-1989 | 26(266) |
| 21-May-2016 | Ben Youngs | Leicester Tigers | England | 05-Sep-1989 | 26(259) |
| 21-May-2016 | Graham Kitchener | Leicester Tigers | England | 29-Sep-1989 | 26(235) |
| 28-May-2016 | Will Fraser | Saracens | England | 29-Oct-1989 | 26(212) |
| 21-May-2016 | Jake Cooper-Woolley | London Wasps | England | 18-Nov-1989 | 26(185) |
| 28-May-2016 | George Kruis | Saracens | England | 22-Feb-1990 | 26(96) |
| 21-May-2016 | Locky McCaffrey | Leicester Tigers | Australia | 19-Mar-1990 | 26(64) |
| 28-May-2016 | Dave Ewers | Exeter Chiefs | England | 03-Nov-1990 | 25(207) |
| 21-May-2016 | Telusa Veainu | Leicester Tigers | Tonga | 26-Dec-1990 | 25(147) |
| 28-May-2016 | Makovina Vunipola | Saracens | England | 13-Jan-1991 | 25(136) |
| 21-May-2016 | Joe Launchberry | London Wasps | England | 12-Apr-1991 | 25(40) |
| 21-May-2016 | Christian Wade | London Wasps | England | 15-May-1991 | 25(7) |
| 21-May-2016 | Manu Tuilagi | Leicester Tigers | England | 18-May-1991 | 25(4) |
| 21-May-2016 | Nathan Hughes | London Wasps | England | 10-Jun-1991 | 24(346) |
| 28-May-2016 | Owen Farrell | Saracens | England | 21-Sep-1991 | 24(250) |
| 28-May-2016 | Harry Williams | Exeter Chiefs | England | 01-Oct-1991 | 24(240) |
| 21-May-2016 | Charles Piutau | London Wasps | New Zealand | 31-Oct-1991 | 24(203) |
| 28-May-2016 | Olly Woodburn | Exeter Chiefs | England | 18-Nov-1991 | 24(192) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).