| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 12-May-2012 |  George Ford | Leicester Tigers | England | 16-Mar-1993 | 19(57) |
| 12-May-2012 |  Owen Farrell | Saracens | England | 21-Sep-1991 | 20(234) |
| 12-May-2012 | Manu Tuilagi | Leicester Tigers | England | 18-May-1991 | 20(360) |
| 12-May-2012 | Jackson Wray | Saracens | England | 10-Nov-1990 | 21(184) |
| 12-May-2012 |  Joe Marler | Harlequins | England | 07-Jul-1990 | 21(310) |
| 12-May-2012 | Sam Smith | Harlequins | England | 06-Jun-1990 | 21(341) |
| 12-May-2012 | Steve Mafi | Leicester Tigers | Tonga | 09-Dec-1989 | 22(155) |
| 12-May-2012 | Will Fraser | Saracens | England | 29-Oct-1989 | 22(196) |
| 12-May-2012 | George Lowe | Harlequins | England | 22-Oct-1989 | 22(203) |
| 12-May-2012 | Ben Youngs | Leicester Tigers | England | 05-Sep-1989 | 22(250) |
| 12-May-2012 |  James Craig | Northampton Saints | England | 08-Nov-1988 | 23(185) |
| 12-May-2012 | Joe Gray | Harlequins | England | 05-Aug-1988 | 23(280) |
| 12-May-2012 | Alex Goode | Saracens | England | 07-May-1988 | 24(5) |
| 12-May-2012 | Jordan Turner-Hall | Harlequins | England | 05-Jan-1988 | 24(128) |
| 12-May-2012 | Vasily Artemyev | Northampton Saints | Russia | 24-Jul-1987 | 24(293) |
| 12-May-2012 | Dan Cole | Leicester Tigers | England | 09-May-1987 | 25(4) |
| 12-May-2012 | Mark Sorenson | Northampton Saints | New Zealand | 12-Feb-1987 | 25(90) |
| 26-May-2012 | Danny Care | Harlequins | England | 02-Jan-1987 | 25(145) |
| 12-May-2012 | Rhys Gill | Saracens | Wales | 30-Oct-1986 | 25(195) |
| 12-May-2012 | Anthony Allen | Leicester Tigers | England | 01-Sep-1986 | 25(254) |
| 12-May-2012 | Bradley Barritt | Saracens | England | 07-Aug-1986 | 25(279) |
| 12-May-2012 | George Pisi | Northampton Saints | Samoa | 29-Jun-1986 | 25(318) |
| 12-May-2012 | Chris Robshaw | Harlequins | England | 04-Jun-1986 | 25(343) |
| 12-May-2012 | Ryan Lamb | Northampton Saints | England | 18-May-1986 | 25(360) |
| 12-May-2012 | James Johnston | Harlequins | Samoa | 06-Mar-1986 | 26(68) |
| 12-May-2012 | George Robson | Harlequins | England | 04-Nov-1985 | 26(190) |
| 26-May-2012 | Julian Salvi | Leicester Tigers | Australia | 09-Nov-1985 | 26(199) |
| 12-May-2012 | Mike Brown | Harlequins | England | 04-Sep-1985 | 26(251) |
| 12-May-2012 | Ben Foden | Northampton Saints | England | 22-Jul-1985 | 26(295) |
| 12-May-2012 | Lee Dickson | Northampton Saints | England | 29-Mar-1985 | 27(44) |
| 12-May-2012 | Paul Diggin | Northampton Saints | England | 23-Jan-1985 | 27(109) |
| 12-May-2012 | Horacio Agulla | Leicester Tigers | Argentina | 20-Oct-1984 | 27(204) |
| 12-May-2012 | Brian Mujati | Northampton Saints | South Africa | 28-Sep-1984 | 27(226) |
| 12-May-2012 | Geoffrey Parling | Leicester Tigers | England | 28-Oct-1983 | 28(197) |
| 12-May-2012 | Tom Williams | Harlequins | England | 12-Oct-1983 | 28(213) |
| 12-May-2012 | Chris Wyles | Saracens | United States | 13-Sep-1983 | 28(242) |
| 12-May-2012 | David Strettle | Saracens | England | 23-Jul-1983 | 28(294) |
| 12-May-2012 | Christian Day | Northampton Saints | England | 24-Jun-1983 | 28(323) |
| 12-May-2012 | Thomas Waldron | Leicester Tigers | England | 28-Apr-1983 | 29(15) |
| 26-May-2012 | Ugo Monye | Harlequins | England | 13-Apr-1983 | 29(44) |
| 12-May-2012 | Marcos Ayerza | Leicester Tigers | Argentina | 12-Jan-1983 | 29(121) |
| 12-May-2012 | George Skivington | Leicester Tigers | England | 03-Dec-1982 | 29(161) |
| 12-May-2012 | Matthew Stevens | Saracens | England | 01-Oct-1982 | 29(224) |
| 12-May-2012 | Karl Dickson | Harlequins | England | 02-Aug-1982 | 29(284) |
| 12-May-2012 | Mouritz Botha | Saracens | England | 29-Jan-1982 | 30(104) |
| 12-May-2012 | Soane Tonga'uiha | Northampton Saints | Tonga | 21-Jan-1982 | 30(112) |
| 12-May-2012 | Phil Dowson | Northampton Saints | England | 01-Oct-1981 | 30(224) |
| 12-May-2012 | Roger Wilson | Northampton Saints | Ireland | 21-Sep-1981 | 30(234) |
| 12-May-2012 | Olly Kohn | Harlequins | Wales | 19-May-1981 | 30(359) |
| 12-May-2012 | Schalk Brits | Saracens | South Africa | 16-May-1981 | 30(362) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).