| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 26-May-2012 |  George Chuter | Leicester Tigers | England | 09-Jul-1976 | 35(322) |
| 12-May-2012 |  Andy Long | Northampton Saints | England | 02-Sep-1977 | 34(253) |
| 26-May-2012 | Geordan Murphy | Leicester Tigers | Ireland | 19-Apr-1978 | 34(38) |
| 26-May-2012 |  Nick Easter | Harlequins | England | 15-Aug-1978 | 33(285) |
| 12-May-2012 |  Neil de Koch | Saracens | South Africa | 20-Nov-1978 | 33(174) |
| 12-May-2012 | Craig Newby | Leicester Tigers | New Zealand | 27-Jul-1979 | 32(290) |
| 12-May-2012 | Steve Borthwick | Saracens | England | 12-Oct-1979 | 32(213) |
| 26-May-2012 | Maurie Fa'asavlu | Harlequins | Samoa | 12-Jan-1980 | 32(135) |
| 26-May-2012 | Nick Evans | Harlequins | New Zealand | 14-Aug-1980 | 31(286) |
| 12-May-2012 | Ernst Joubert | Saracens | South Africa | 05-Aug-1980 | 31(281) |
| 12-May-2012 | Charlie Hodgson | Saracens | England | 12-Nov-1980 | 31(181) |
| 26-May-2012 | Alesana Tuilagi | Leicester Tigers | Samoa | 24-Feb-1981 | 31(91) |
| 12-May-2012 | James Downey | Northampton Saints | Ireland | 23-Mar-1981 | 31(51) |
| 26-May-2012 | Olly Kohn | Harlequins | Wales | 19-May-1981 | 31(7) |
| 12-May-2012 | Schalk Brits | Saracens | South Africa | 16-May-1981 | 30(362) |
| 12-May-2012 | Roger Wilson | Northampton Saints | Ireland | 21-Sep-1981 | 30(234) |
| 12-May-2012 | Phil Dowson | Northampton Saints | England | 01-Oct-1981 | 30(224) |
| 12-May-2012 | Soane Tonga'uiha | Northampton Saints | Tonga | 21-Jan-1982 | 30(112) |
| 12-May-2012 | Mouritz Botha | Saracens | England | 29-Jan-1982 | 30(104) |
| 12-May-2012 | Karl Dickson | Harlequins | England | 02-Aug-1982 | 29(284) |
| 12-May-2012 | Matthew Stevens | Saracens | England | 01-Oct-1982 | 29(224) |
| 26-May-2012 | George Skivington | Leicester Tigers | England | 03-Dec-1982 | 29(175) |
| 26-May-2012 | Marcos Ayerza | Leicester Tigers | Argentina | 12-Jan-1983 | 29(135) |
| 26-May-2012 | Ugo Monye | Harlequins | England | 13-Apr-1983 | 29(44) |
| 26-May-2012 | Thomas Waldron | Leicester Tigers | England | 28-Apr-1983 | 29(29) |
| 12-May-2012 | Christian Day | Northampton Saints | England | 24-Jun-1983 | 28(323) |
| 12-May-2012 | David Strettle | Saracens | England | 23-Jul-1983 | 28(294) |
| 12-May-2012 | Chris Wyles | Saracens | United States | 13-Sep-1983 | 28(242) |
| 26-May-2012 | Tom Williams | Harlequins | England | 12-Oct-1983 | 28(227) |
| 26-May-2012 | Geoffrey Parling | Leicester Tigers | England | 28-Oct-1983 | 28(211) |
| 12-May-2012 | Brian Mujati | Northampton Saints | South Africa | 28-Sep-1984 | 27(226) |
| 26-May-2012 | Horacio Agulla | Leicester Tigers | Argentina | 20-Oct-1984 | 27(218) |
| 12-May-2012 | Paul Diggin | Northampton Saints | England | 23-Jan-1985 | 27(109) |
| 12-May-2012 | Lee Dickson | Northampton Saints | England | 29-Mar-1985 | 27(44) |
| 12-May-2012 | Ben Foden | Northampton Saints | England | 22-Jul-1985 | 26(295) |
| 26-May-2012 | Mike Brown | Harlequins | England | 04-Sep-1985 | 26(265) |
| 26-May-2012 | George Robson | Harlequins | England | 04-Nov-1985 | 26(204) |
| 26-May-2012 | Julian Salvi | Leicester Tigers | Australia | 09-Nov-1985 | 26(199) |
| 26-May-2012 | James Johnston | Harlequins | Samoa | 06-Mar-1986 | 26(82) |
| 12-May-2012 | Ryan Lamb | Northampton Saints | England | 18-May-1986 | 25(360) |
| 26-May-2012 | Chris Robshaw | Harlequins | England | 04-Jun-1986 | 25(357) |
| 12-May-2012 | George Pisi | Northampton Saints | Samoa | 29-Jun-1986 | 25(318) |
| 12-May-2012 | Bradley Barritt | Saracens | England | 07-Aug-1986 | 25(279) |
| 26-May-2012 | Anthony Allen | Leicester Tigers | England | 01-Sep-1986 | 25(268) |
| 12-May-2012 | Rhys Gill | Saracens | Wales | 30-Oct-1986 | 25(195) |
| 26-May-2012 | Danny Care | Harlequins | England | 02-Jan-1987 | 25(145) |
| 12-May-2012 | Mark Sorenson | Northampton Saints | New Zealand | 12-Feb-1987 | 25(90) |
| 26-May-2012 | Dan Cole | Leicester Tigers | England | 09-May-1987 | 25(18) |
| 12-May-2012 | Vasily Artemyev | Northampton Saints | Russia | 24-Jul-1987 | 24(293) |
| 26-May-2012 | Jordan Turner-Hall | Harlequins | England | 05-Jan-1988 | 24(142) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).