| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 08-Nov-2009 |  George Ford | Leicester Tigers | England | 16-Mar-1993 | 16(237) |
| 29-Jan-2010 |  Tom Prydie | Ospreys | Wales | 23-Feb-1992 | 17(341) |
| 08-Nov-2009 | Alex Lewington | Leicester Tigers | England | 20-Sep-1991 | 18(50) |
| 08-Nov-2009 | Joe Cobden | Leicester Tigers | England | 01-Sep-1991 | 18(69) |
| 15-Nov-2009 |  Jonathan Joseph | London Irish | England | 21-May-1991 | 18(179) |
| 15-Nov-2009 |  Jack Wallace | London Wasps | England | 22-Jan-1991 | 18(298) |
| 08-Nov-2009 | Matt Everard | Leicester Tigers | England | 09-Jan-1991 | 18(304) |
| 04-Feb-2010 | Hanno Dirksen | Ospreys | South Africa | 31-Mar-1991 | 18(311) |
| 15-Nov-2009 |  Josh Navidi | Cardiff Blues | Wales | 30-Dec-1990 | 18(321) |
| 13-Nov-2009 | Gareth Denman | Leeds Tykes | England | 12-Nov-1990 | 19(1) |
| 14-Nov-2009 |  Jackson Wray | Saracens | England | 10-Nov-1990 | 19(4) |
| 07-Nov-2009 |  Tom Catterick | Newcastle Falcons | England | 18-Oct-1990 | 19(20) |
| 15-Nov-2009 |  Ross Chisholm | Harlequins | England | 05-Oct-1990 | 19(41) |
| 08-Nov-2009 | Tom Armes | Leicester Tigers | England | 19-Sep-1990 | 19(50) |
| 05-Nov-2009 | Kristian Phillips | Ospreys | Wales | 02-Sep-1990 | 19(64) |
| 14-Nov-2009 | Andrew Forsyth | Leicester Tigers | England | 09-Sep-1990 | 19(66) |
| 14-Nov-2009 | James King | Ospreys | Wales | 24-Jul-1990 | 19(113) |
| 08-Nov-2009 | Joe Ford | Leeds Tykes | England | 04-Jun-1990 | 19(157) |
| 08-Nov-2009 |  Tavis Knoyle | Scarlets | Wales | 02-Jun-1990 | 19(159) |
| 15-Nov-2009 | Sam Smith | Harlequins | England | 06-Jun-1990 | 19(162) |
| 06-Nov-2009 |  James Gaskell | Sale Sharks | England | 20-May-1990 | 19(170) |
| 08-Nov-2009 |  Freddie Burns | Gloucester | England | 13-May-1990 | 19(179) |
| 29-Jan-2010 | Callum Green | Leicester Tigers | England | 15-Jul-1990 | 19(198) |
| 14-Nov-2009 | Ashley Beck | Ospreys | Wales | 15-Apr-1990 | 19(213) |
| 08-Nov-2009 | Tom Homer | London Irish | England | 01-Apr-1990 | 19(221) |
| 15-Nov-2009 | Jonny May | Gloucester | England | 01-Apr-1990 | 19(228) |
| 06-Nov-2009 | Dan Watchurst | Gwent Dragons | Wales | 15-Mar-1990 | 19(236) |
| 14-Nov-2009 | George Kruis | Saracens | England | 22-Feb-1990 | 19(265) |
| 31-Jan-2010 | Michael Tait | Newcastle Falcons | Scotland | 07-May-1990 | 19(269) |
| 30-Jan-2010 | Yann Thomas | Gloucester | England | 16-Apr-1990 | 19(289) |
| 08-Nov-2009 | Rhys Williams | Cardiff Blues | Wales | 16-Jan-1990 | 19(296) |
| 08-Nov-2009 | Will Hurrell | Leicester Tigers | England | 15-Jan-1990 | 19(297) |
| 31-Jan-2010 | Will Welch | Newcastle Falcons | England | 03-Apr-1990 | 19(303) |
| 08-Nov-2009 | Rory Clegg | Harlequins | England | 06-Jan-1990 | 19(306) |
| 13-Nov-2009 | Alex Ball | Leeds Tykes | England | 09-Jan-1990 | 19(308) |
| 15-Nov-2009 | Chris Yorke | Harlequins | England | 27-Dec-1989 | 19(323) |
| 08-Nov-2009 | Philip Swainston | Leeds Tykes | England | 12-Dec-1989 | 19(331) |
| 06-Feb-2010 | Jordan Davies | Sale Sharks | England | 12-Mar-1990 | 19(331) |
| 06-Feb-2010 | Mark Atkinson | Sale Sharks | England | 08-Mar-1990 | 19(335) |
| 14-Nov-2009 | Guy Mercer | Bath | England | 12-Dec-1989 | 19(337) |
| 29-Jan-2010 | Jonny Kennedy | Sale Sharks | Scotland | 16-Feb-1990 | 19(347) |
| 14-Nov-2009 |  Oliver Frost | Worcester Warriors | England | 02-Dec-1989 | 19(347) |
| 29-Jan-2010 | Jacob Rowan | Leeds Tykes | England | 14-Jan-1990 | 20(15) |
| 15-Nov-2009 | George Lowe | Harlequins | England | 22-Oct-1989 | 20(24) |
| 14-Nov-2009 | James Short | Saracens | England | 15-Oct-1989 | 20(30) |
| 07-Nov-2009 | Mark Wilson | Newcastle Falcons | England | 06-Oct-1989 | 20(32) |
| 14-Nov-2009 | Graham Kitchener | Worcester Warriors | England | 29-Sep-1989 | 20(46) |
| 08-Nov-2009 | Bob Baker | London Wasps | England | 12-Sep-1989 | 20(57) |
| 14-Nov-2009 | Ben Youngs | Leicester Tigers | England | 05-Sep-1989 | 20(70) |
| 07-Nov-2009 | Robert Miller | Newcastle Falcons | England | 05-Aug-1989 | 20(94) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).