| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 05-Nov-2009 |  Filo Tiatia | Ospreys | New Zealand | 04-Jun-1971 | 38(155) |
| 06-Feb-2010 |  Mike Catt | London Irish | England | 17-Sep-1971 | 38(143) |
| 30-Jan-2010 |  Olivier Sourgens | Worcester Warriors | France | 17-Jan-1972 | 38(14) |
| 06-Feb-2010 |  Julian White | Leicester Tigers | England | 14-May-1973 | 36(268) |
| 08-Nov-2009 | Alejandro Moreno | Leeds Tykes | Italy | 21-Apr-1973 | 36(201) |
| 07-Feb-2010 |  Justin Marshall | Saracens | New Zealand | 05-Aug-1973 | 36(186) |
| 15-Nov-2009 |  Simon Shaw | London Wasps | England | 01-Sep-1973 | 36(75) |
| 21-Mar-2010 |  Pierre Capdevielle | Gloucester | France | 30-Mar-1974 | 35(357) |
| 29-Jan-2010 | Henry Paul | Leeds Tykes | England | 10-Feb-1974 | 35(354) |
| 06-Feb-2010 | Tevita Taumoepeau | Worcester Warriors | Tonga | 16-May-1974 | 35(267) |
| 31-Jan-2010 |  Maama Molitika | Cardiff Blues | Tonga | 26-Aug-1974 | 35(159) |
| 21-Mar-2010 | Olivier Azam | Gloucester | France | 21-Oct-1974 | 35(152) |
| 06-Feb-2010 | Duncan Bell | Bath | England | 01-Oct-1974 | 35(129) |
| 14-Mar-2010 |  Ignacio Fernandez Lobbe | Northampton Saints | Argentina | 20-Nov-1974 | 35(115) |
| 15-Nov-2009 | Gareth Thomas | Cardiff Blues | Wales | 25-Jul-1974 | 35(114) |
| 08-Nov-2009 | Darren Morris | Cardiff Blues | Wales | 24-Sep-1974 | 35(46) |
| 06-Feb-2010 | Andy Perry | London Irish | England | 28-Dec-1974 | 35(41) |
| 06-Feb-2010 | Adam Black | Worcester Warriors | Wales | 24-May-1975 | 34(259) |
| 29-Jan-2010 | Mosese Raulini | Saracens | Fiji | 27-Jun-1975 | 34(217) |
| 14-Nov-2009 | Santigo Bonorino | Northampton Saints | Argentina | 05-May-1975 | 34(194) |
| 31-Jan-2010 | Dafydd James | Cardiff Blues | Wales | 24-Jul-1975 | 34(192) |
| 07-Feb-2010 | Mark Robinson | London Wasps | New Zealand | 21-Aug-1975 | 34(171) |
| 06-Feb-2010 |  Oriol Ripol | Sale Sharks | Spain | 06-Sep-1975 | 34(154) |
| 06-Feb-2010 | Chris Latham | Worcester Warriors | Australia | 08-Sep-1975 | 34(152) |
| 14-Mar-2010 | Jake Boer | Gloucester | South Africa | 01-Nov-1975 | 34(134) |
| 07-Feb-2010 | Glen Jackson | Saracens | New Zealand | 23-Oct-1975 | 34(108) |
| 14-Nov-2009 | Chris Fortey | Worcester Warriors | England | 25-Aug-1975 | 34(82) |
| 06-Feb-2010 | Ben Kay | Leicester Tigers | England | 14-Dec-1975 | 34(55) |
| 06-Feb-2010 | Alan Dickens | Northampton Saints | England | 04-Feb-1976 | 34(3) |
| 14-Nov-2009 | Shaun Connor | Gwent Dragons | Wales | 20-Nov-1975 | 33(360) |
| 06-Feb-2010 | Faan Rautenbach | London Irish | South Africa | 22-Feb-1976 | 33(350) |
| 21-Mar-2010 | Bruce Reihana | Northampton Saints | New Zealand | 06-Apr-1976 | 33(349) |
| 06-Nov-2009 | Danny Lee | Gwent Dragons | New Zealand | 01-Feb-1976 | 33(279) |
| 06-Feb-2010 | Craig Gillies | Worcester Warriors | England | 06-May-1976 | 33(276) |
| 06-Feb-2010 | David Barnes | Bath | England | 12-Jul-1976 | 33(209) |
| 14-Nov-2009 | Carlos Nieto | Saracens | Italy | 28-Apr-1976 | 33(200) |
| 29-Jan-2010 | Hugh Vyvyan | Saracens | England | 08-Sep-1976 | 33(143) |
| 29-Jan-2010 | Nicky Little | Bath | Fiji | 13-Sep-1976 | 33(138) |
| 14-Nov-2009 | George Chuter | Leicester Tigers | England | 09-Jul-1976 | 33(128) |
| 14-Mar-2010 | Kas Lealamanu'a | Saracens | Samoa | 15-Dec-1976 | 33(89) |
| 21-Mar-2010 | William James | Gloucester | Wales | 22-Dec-1976 | 33(89) |
| 05-Nov-2009 | Ian Gough | Ospreys | Wales | 10-Nov-1976 | 32(360) |
| 29-Jan-2010 | Shane Williams | Ospreys | Wales | 26-Feb-1977 | 32(337) |
| 06-Feb-2010 | Gavin Kerr | Sale Sharks | Scotland | 03-Apr-1977 | 32(309) |
| 07-Feb-2010 | Steve Jones | Gwent Dragons | Wales | 20-Apr-1977 | 32(293) |
| 14-Mar-2010 | Taufa'ao Filise | Cardiff Blues | Tonga | 26-May-1977 | 32(292) |
| 14-Nov-2009 | Kevin Sorrell | Saracens | England | 06-Mar-1977 | 32(253) |
| 14-Mar-2010 | Xavier Rush | Cardiff Blues | New Zealand | 13-Jul-1977 | 32(244) |
| 07-Feb-2010 |  Ceri Jones | Harlequins | Wales | 19-Jun-1977 | 32(233) |
| 30-Jan-2010 | Joe Worsley | London Wasps | England | 14-Jun-1977 | 32(230) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).