| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 08-May-2010 |  Jonathan Joseph | London Irish | England | 21-May-1991 | 18(353) |
| 08-May-2010 |  Louis Silver | Worcester Warriors | England | 10-Feb-1991 | 19(87) |
| 04-Sep-2009 |  James Gaskell | Sale Sharks | England | 20-May-1990 | 19(107) |
| 31-Oct-2009 |  Callum Green | Leicester Tigers | England | 15-Jul-1990 | 19(108) |
| 14-Feb-2010 | Jamie Gibson | London Irish | England | 27-Sep-1990 | 19(140) |
| 28-Nov-2009 |  Joe Ford | Leeds Tykes | England | 04-Jun-1990 | 19(177) |
| 20-Nov-2009 |  Freddie Burns | Gloucester | England | 13-May-1990 | 19(191) |
| 24-Oct-2009 |  Will Welch | Newcastle Falcons | England | 03-Apr-1990 | 19(204) |
| 24-Oct-2009 | Tom Homer | London Irish | England | 01-Apr-1990 | 19(206) |
| 08-May-2010 | Lee Imiolek | Sale Sharks | England | 21-Sep-1990 | 19(229) |
| 23-Apr-2010 | Kieran Brookes | Newcastle Falcons | England | 29-Aug-1990 | 19(237) |
| 28-Feb-2010 |  Sam Smith | Harlequins | England | 06-Jun-1990 | 19(267) |
| 31-Oct-2009 | Rory Clegg | Harlequins | England | 06-Jan-1990 | 19(298) |
| 27-Dec-2009 | Jonny Kennedy | Sale Sharks | Scotland | 16-Feb-1990 | 19(314) |
| 20-Feb-2010 | Jonny May | Gloucester | England | 01-Apr-1990 | 19(325) |
| 12-Sep-2009 | George Lowe | Harlequins | England | 22-Oct-1989 | 19(325) |
| 04-Sep-2009 | Ben Youngs | Leicester Tigers | England | 05-Sep-1989 | 19(364) |
| 06-Sep-2009 | Charles Sharples | Gloucester | England | 17-Aug-1989 | 20(20) |
| 19-Sep-2009 | Robert Miller | Newcastle Falcons | England | 05-Aug-1989 | 20(45) |
| 13-Feb-2010 | Chris Yorke | Harlequins | England | 27-Dec-1989 | 20(48) |
| 03-Jan-2010 |  Bob Baker | London Wasps | England | 12-Sep-1989 | 20(113) |
| 19-Sep-2009 | David Lewis | Gloucester | England | 29-Apr-1989 | 20(143) |
| 26-Feb-2010 | Graham Kitchener | Worcester Warriors | England | 29-Sep-1989 | 20(150) |
| 30-Oct-2009 | Carl Fearns | Sale Sharks | England | 25-May-1989 | 20(158) |
| 22-Nov-2009 | Calum Clark | Leeds Tykes | England | 10-Jun-1989 | 20(165) |
| 20-Nov-2009 | Ben Williams | Bath | England | 28-May-1989 | 20(176) |
| 06-Sep-2009 |  Courtney Lawes | Northampton Saints | England | 23-Feb-1989 | 20(195) |
| 23-Apr-2010 | Grant Shiells | Newcastle Falcons | Scotland | 23-Sep-1989 | 20(212) |
| 20-Nov-2009 | Henry Trinder | Gloucester | England | 14-Apr-1989 | 20(220) |
| 06-Sep-2009 | Michael Young | Newcastle Falcons | England | 31-Dec-1988 | 20(249) |
| 05-Dec-2009 | John Arr | Worcester Warriors | England | 29-Nov-1988 | 21(6) |
| 05-Sep-2009 |  Andy Saull | Saracens | England | 27-Aug-1988 | 21(9) |
| 31-Oct-2009 | James Cannon | Northampton Saints | England | 24-Sep-1988 | 21(37) |
| 05-Sep-2009 | Joe Simpson | London Wasps | England | 05-Jul-1988 | 21(62) |
| 28-Nov-2009 | Alex Corbisiero | London Irish | England | 30-Aug-1988 | 21(90) |
| 14-Feb-2010 | Billy Twelvetrees | Leicester Tigers | England | 15-Nov-1988 | 21(91) |
| 06-Sep-2009 | Miles Benjamin | Worcester Warriors | England | 11-May-1988 | 21(118) |
| 05-Sep-2009 | Alex Goode | Saracens | England | 07-May-1988 | 21(121) |
| 06-Sep-2009 | Greg King | Worcester Warriors | England | 24-Apr-1988 | 21(135) |
| 05-Sep-2009 | Noah Cato | Saracens | England | 31-Mar-1988 | 21(158) |
| 06-Sep-2009 | Alex Tait | Newcastle Falcons | England | 18-Mar-1988 | 21(172) |
| 26-Sep-2009 | Tom Denton | Leeds Tykes | England | 13-Feb-1988 | 21(226) |
| 05-Sep-2009 | Jordan Turner-Hall | Harlequins | England | 05-Jan-1988 | 21(244) |
| 01-Nov-2009 | Jacob Abbott | Worcester Warriors | England | 04-Feb-1988 | 21(271) |
| 06-Sep-2009 | Alex Grove | Worcester Warriors | Scotland | 30-Nov-1987 | 21(281) |
| 20-Sep-2009 | Luther Burrell | Leeds Tykes | England | 06-Dec-1987 | 21(289) |
| 05-Sep-2009 | Danny Cipriani | London Wasps | England | 02-Nov-1987 | 21(308) |
| 06-Sep-2009 | Peter Browne | Newcastle Falcons | England | 25-Oct-1987 | 21(317) |
| 20-Sep-2009 | Lachlan Mitchell | London Wasps | Australia | 30-Sep-1987 | 21(356) |
| 20-Sep-2009 | Dominic Waldouck | London Wasps | England | 26-Sep-1987 | 21(360) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).