| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 08-May-2010 |  Mike Catt | London Irish | England | 17-Sep-1971 | 38(234) |
| 08-May-2010 |  Olivier Sourgens | Worcester Warriors | France | 17-Jan-1972 | 38(112) |
| 24-Apr-2010 |  Mefin Davies | Leicester Tigers | Wales | 02-Sep-1972 | 37(234) |
| 08-May-2010 |  Danny Grewcock | Bath | England | 07-Nov-1972 | 37(182) |
| 27-Feb-2010 | Julian White | Leicester Tigers | England | 14-May-1973 | 36(289) |
| 10-Apr-2010 |  Tani Fuga | Harlequins | Samoa | 14-Jul-1973 | 36(270) |
| 17-Apr-2010 |  Justin Marshall | Saracens | New Zealand | 05-Aug-1973 | 36(255) |
| 24-Apr-2010 |  Simon Shaw | London Wasps | England | 01-Sep-1973 | 36(235) |
| 08-May-2010 | Serge Betsen | London Wasps | France | 25-Mar-1974 | 36(44) |
| 28-Feb-2010 | Henry Paul | Leeds Tykes | England | 10-Feb-1974 | 36(18) |
| 31-Mar-2010 |  Pierre Capdevielle | Gloucester | France | 30-Mar-1974 | 36(1) |
| 25-Apr-2010 | Tevita Taumoepeau | Worcester Warriors | Tonga | 16-May-1974 | 35(345) |
| 08-May-2010 | Andy Gomersall | Leeds Tykes | England | 24-Jul-1974 | 35(289) |
| 24-Apr-2010 | Duncan Bell | Bath | England | 01-Oct-1974 | 35(206) |
| 17-Apr-2010 | Olivier Azam | Gloucester | France | 21-Oct-1974 | 35(179) |
| 08-May-2010 |  Ignacio Fernandez Lobbe | Northampton Saints | Argentina | 20-Nov-1974 | 35(170) |
| 28-Mar-2010 |  Brent Cockbain | Sale Sharks | Wales | 15-Nov-1974 | 35(134) |
| 08-May-2010 | Adam Black | Worcester Warriors | Wales | 24-May-1975 | 34(350) |
| 05-Sep-2009 | Andy Perry | London Irish | England | 28-Dec-1974 | 34(252) |
| 28-Mar-2010 | Dafydd James | Sale Sharks | Wales | 24-Jul-1975 | 34(248) |
| 08-May-2010 | Oriol Ripol | Sale Sharks | Spain | 06-Sep-1975 | 34(245) |
| 08-May-2010 | Chris Latham | Worcester Warriors | Australia | 08-Sep-1975 | 34(243) |
| 02-Apr-2010 | Chris Fortey | Worcester Warriors | England | 25-Aug-1975 | 34(221) |
| 27-Nov-2009 | Santigo Bonorino | Northampton Saints | Argentina | 05-May-1975 | 34(207) |
| 08-May-2010 | Glen Jackson | Saracens | New Zealand | 23-Oct-1975 | 34(198) |
| 17-Apr-2010 | Jake Boer | Gloucester | South Africa | 01-Nov-1975 | 34(168) |
| 05-Dec-2009 | Mosese Raulini | Saracens | Fiji | 27-Jun-1975 | 34(162) |
| 08-May-2010 | Ben Kay | Leicester Tigers | England | 14-Dec-1975 | 34(146) |
| 09-Jan-2010 | Mark Robinson | London Wasps | New Zealand | 21-Aug-1975 | 34(142) |
| 08-May-2010 | Faan Rautenbach | London Irish | South Africa | 22-Feb-1976 | 34(76) |
| 20-Apr-2010 | Alan Dickens | Northampton Saints | England | 04-Feb-1976 | 34(76) |
| 08-May-2010 | Philip Vickery | London Wasps | England | 14-Mar-1976 | 34(55) |
| 20-Apr-2010 | Barry Everitt | Northampton Saints | Ireland | 09-Mar-1976 | 34(42) |
| 08-May-2010 | Bruce Reihana | Northampton Saints | New Zealand | 06-Apr-1976 | 34(32) |
| 24-Oct-2009 | Carlos Spencer | Gloucester | New Zealand | 14-Oct-1975 | 34(11) |
| 08-May-2010 | Craig Gillies | Worcester Warriors | England | 06-May-1976 | 34(2) |
| 08-May-2010 | George Chuter | Leicester Tigers | England | 09-Jul-1976 | 33(303) |
| 24-Apr-2010 | David Barnes | Bath | England | 12-Jul-1976 | 33(286) |
| 02-Jan-2010 | Carlos Nieto | Saracens | Italy | 28-Apr-1976 | 33(249) |
| 08-May-2010 | Hugh Vyvyan | Saracens | England | 08-Sep-1976 | 33(242) |
| 20-Apr-2010 | Nicky Little | Bath | Fiji | 13-Sep-1976 | 33(219) |
| 02-Jan-2010 | Rob Rawlinson | Leeds Tykes | England | 23-Aug-1976 | 33(132) |
| 03-Apr-2010 | William James | Gloucester | Wales | 22-Dec-1976 | 33(102) |
| 28-Feb-2010 | Kas Lealamanu'a | Saracens | Samoa | 15-Dec-1976 | 33(75) |
| 28-Mar-2010 | Gavin Kerr | Sale Sharks | Scotland | 03-Apr-1977 | 32(359) |
| 08-May-2010 | Ceri Jones | Harlequins | Wales | 19-Jun-1977 | 32(323) |
| 24-Apr-2010 | Joe Worsley | London Wasps | England | 14-Jun-1977 | 32(314) |
| 08-May-2010 | Dale Rasmussen | Worcester Warriors | Samoa | 06-Jul-1977 | 32(306) |
| 24-Apr-2010 | Mark van Gisbergen | London Wasps | England | 30-Jun-1977 | 32(298) |
| 08-May-2010 | Danie Coetzee | London Irish | South Africa | 02-Sep-1977 | 32(248) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).