| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 09-May-2009 |  Jordan Turner-Hall | Harlequins | England | 05-Jan-1988 | 21(125) |
| 09-May-2009 |  Adam Thompstone | London Irish | England | 04-Sep-1987 | 21(248) |
| 09-May-2009 | Danny Care | Harlequins | England | 02-Jan-1987 | 22(128) |
| 09-May-2009 |  Matt Banahan | Bath | England | 30-Dec-1986 | 22(131) |
| 09-May-2009 | Nick Abendanon | Bath | England | 27-Aug-1986 | 22(256) |
| 09-May-2009 | Chris Robshaw | Harlequins | England | 04-Jun-1986 | 22(340) |
| 09-May-2009 |  Jordan Crane | Leicester Tigers | England | 03-Jun-1986 | 22(341) |
| 09-May-2009 | Ryan Davis | Bath | England | 11-Nov-1985 | 23(179) |
| 09-May-2009 | Tom Croft | Leicester Tigers | England | 07-Nov-1985 | 23(183) |
| 09-May-2009 | George Robson | Harlequins | England | 04-Nov-1985 | 23(186) |
| 09-May-2009 | Steffon Armitage | London Irish | England | 20-Sep-1985 | 23(231) |
| 09-May-2009 | Mike Brown | Harlequins | England | 04-Sep-1985 | 23(247) |
| 09-May-2009 | Gonzalo Tiesi | Harlequins | Argentina | 25-Apr-1985 | 24(14) |
| 09-May-2009 | Johne Murphy | Leicester Tigers | Ireland | 10-Nov-1984 | 24(180) |
| 09-May-2009 | Will Skinner | Harlequins | England | 08-Feb-1984 | 25(91) |
| 09-May-2009 | Julian Dupuy | Leicester Tigers | France | 19-Dec-1983 | 25(142) |
| 09-May-2009 | Delon Armitage | London Irish | England | 15-Dec-1983 | 25(146) |
| 09-May-2009 | Tom Williams | Harlequins | England | 12-Oct-1983 | 25(210) |
| 09-May-2009 | Ugo Monye | Harlequins | England | 13-Apr-1983 | 26(27) |
| 09-May-2009 | James Percival | Harlequins | England | 09-Feb-1983 | 26(90) |
| 09-May-2009 | Marcos Ayerza | Leicester Tigers | Argentina | 12-Jan-1983 | 26(118) |
| 09-May-2009 | Sailosi Tagicakibau | London Irish | Samoa | 14-Nov-1982 | 26(177) |
| 09-May-2009 | Ben Woods | Leicester Tigers | England | 09-Jun-1982 | 26(335) |
| 09-May-2009 | Dan Hipkiss | Leicester Tigers | England | 04-Jun-1982 | 26(340) |
| 09-May-2009 | Paul Hodgson | London Irish | England | 25-Apr-1982 | 27(14) |
| 09-May-2009 | Shontayne Hape | Bath | England | 30-Jan-1982 | 27(99) |
| 09-May-2009 | Michael Claassens | Bath | South Africa | 29-Jan-1982 | 27(100) |
| 09-May-2009 | James Scayesbrook | Bath | England | 01-Jan-1982 | 27(128) |
| 09-May-2009 | Sam Vesty | Leicester Tigers | England | 26-Nov-1981 | 27(164) |
| 09-May-2009 | Alex Crockett | Bath | England | 20-Nov-1981 | 27(170) |
| 09-May-2009 | Stuart Hooper | Bath | England | 18-Nov-1981 | 27(172) |
| 09-May-2009 | Martin Castrogiovanni | Leicester Tigers | Italy | 21-Oct-1981 | 27(200) |
| 16-May-2009 | James Hudson | London Irish | England | 28-Oct-1981 | 27(200) |
| 09-May-2009 | Nick Kennedy | London Irish | England | 19-Aug-1981 | 27(263) |
| 09-May-2009 | Richard Skuse | London Irish | England | 11-Sep-1980 | 28(240) |
| 09-May-2009 | Nick Evans | Harlequins | New Zealand | 14-Aug-1980 | 28(268) |
| 09-May-2009 | Clarke Dermody | London Irish | New Zealand | 22-Apr-1980 | 29(17) |
| 09-May-2009 | Scott Hamilton | Leicester Tigers | New Zealand | 04-Mar-1980 | 29(66) |
| 09-May-2009 | Seilala Mapusua | London Irish | Samoa | 27-Feb-1980 | 29(72) |
| 09-May-2009 | Ayoola Erinle | Leicester Tigers | England | 20-Feb-1980 | 29(79) |
| 09-May-2009 | David Flatman | Bath | England | 21-Jan-1980 | 29(109) |
| 09-May-2009 | Declan Danagher | London Irish | England | 11-Jan-1980 | 29(119) |
| 09-May-2009 | Mike Ross | Harlequins | Ireland | 21-Dec-1979 | 29(140) |
| 09-May-2009 | Craig Newby | Leicester Tigers | New Zealand | 27-Jul-1979 | 29(287) |
| 09-May-2009 | Peter Short | Bath | England | 20-Jun-1979 | 29(324) |
| 09-May-2009 | Lee Mears | Bath | England | 05-Mar-1979 | 30(66) |
| 09-May-2009 | Shaun Berne | Bath | Australia | 08-Jan-1979 | 30(122) |
| 09-May-2009 | Joe Maddock | Bath | New Zealand | 20-Dec-1978 | 30(141) |
| 09-May-2009 | Christopher Hala'ufia | London Irish | Tonga | 24-Oct-1978 | 30(198) |
| 09-May-2009 | Andy Beattie | Bath | England | 06-Sep-1978 | 30(246) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).