| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 16-May-2009 |  Mike Catt | London Irish | England | 17-Sep-1971 | 37(242) |
| 16-May-2009 |  Julian White | Leicester Tigers | England | 14-May-1973 | 36(2) |
| 09-May-2009 |  Tani Fuga | Harlequins | Samoa | 14-Jul-1973 | 35(300) |
| 09-May-2009 |  Justin Harrison | Bath | Australia | 20-Apr-1974 | 35(20) |
| 09-May-2009 | Duncan Bell | Bath | England | 01-Oct-1974 | 34(221) |
| 16-May-2009 | Ben Kay | Leicester Tigers | England | 14-Dec-1975 | 33(154) |
| 16-May-2009 | George Chuter | Leicester Tigers | England | 09-Jul-1976 | 32(311) |
| 09-May-2009 | David Barnes | Bath | England | 12-Jul-1976 | 32(301) |
| 09-May-2009 | Ceri Jones | Harlequins | Wales | 19-Jun-1977 | 31(324) |
| 16-May-2009 | Danie Coetzee | London Irish | South Africa | 02-Sep-1977 | 31(257) |
| 16-May-2009 | Peter Hewat | London Irish | Australia | 17-Mar-1978 | 31(60) |
| 16-May-2009 | Geordan Murphy | Leicester Tigers | Ireland | 19-Apr-1978 | 31(28) |
| 16-May-2009 | Bob Casey | London Irish | Ireland | 18-Jul-1978 | 30(303) |
| 09-May-2009 | Nick Easter | Harlequins | England | 15-Aug-1978 | 30(268) |
| 09-May-2009 | Andy Beattie | Bath | England | 06-Sep-1978 | 30(246) |
| 16-May-2009 | Christopher Hala'ufia | London Irish | Tonga | 24-Oct-1978 | 30(205) |
| 09-May-2009 | Joe Maddock | Bath | New Zealand | 20-Dec-1978 | 30(141) |
| 09-May-2009 | Shaun Berne | Bath | Australia | 08-Jan-1979 | 30(122) |
| 09-May-2009 | Lee Mears | Bath | England | 05-Mar-1979 | 30(66) |
| 09-May-2009 | Peter Short | Bath | England | 20-Jun-1979 | 29(324) |
| 16-May-2009 | Craig Newby | Leicester Tigers | New Zealand | 27-Jul-1979 | 29(294) |
| 09-May-2009 | Mike Ross | Harlequins | Ireland | 21-Dec-1979 | 29(140) |
| 16-May-2009 | Declan Danagher | London Irish | England | 11-Jan-1980 | 29(126) |
| 09-May-2009 | David Flatman | Bath | England | 21-Jan-1980 | 29(109) |
| 16-May-2009 | Ayoola Erinle | Leicester Tigers | England | 20-Feb-1980 | 29(86) |
| 16-May-2009 | Seilala Mapusua | London Irish | Samoa | 27-Feb-1980 | 29(79) |
| 16-May-2009 | Scott Hamilton | Leicester Tigers | New Zealand | 04-Mar-1980 | 29(73) |
| 16-May-2009 | Clarke Dermody | London Irish | New Zealand | 22-Apr-1980 | 29(24) |
| 09-May-2009 | Nick Evans | Harlequins | New Zealand | 14-Aug-1980 | 28(268) |
| 16-May-2009 | Richard Skuse | London Irish | England | 11-Sep-1980 | 28(247) |
| 09-May-2009 | Nick Kennedy | London Irish | England | 19-Aug-1981 | 27(263) |
| 09-May-2009 | Martin Castrogiovanni | Leicester Tigers | Italy | 21-Oct-1981 | 27(200) |
| 16-May-2009 | James Hudson | London Irish | England | 28-Oct-1981 | 27(200) |
| 09-May-2009 | Stuart Hooper | Bath | England | 18-Nov-1981 | 27(172) |
| 16-May-2009 | Sam Vesty | Leicester Tigers | England | 26-Nov-1981 | 27(171) |
| 09-May-2009 | Alex Crockett | Bath | England | 20-Nov-1981 | 27(170) |
| 09-May-2009 | James Scayesbrook | Bath | England | 01-Jan-1982 | 27(128) |
| 09-May-2009 | Michael Claassens | Bath | South Africa | 29-Jan-1982 | 27(100) |
| 09-May-2009 | Shontayne Hape | Bath | England | 30-Jan-1982 | 27(99) |
| 16-May-2009 | Paul Hodgson | London Irish | England | 25-Apr-1982 | 27(21) |
| 16-May-2009 | Dan Hipkiss | Leicester Tigers | England | 04-Jun-1982 | 26(347) |
| 16-May-2009 | Ben Woods | Leicester Tigers | England | 09-Jun-1982 | 26(342) |
| 16-May-2009 | Sailosi Tagicakibau | London Irish | Samoa | 14-Nov-1982 | 26(184) |
| 16-May-2009 | Marcos Ayerza | Leicester Tigers | Argentina | 12-Jan-1983 | 26(125) |
| 09-May-2009 | James Percival | Harlequins | England | 09-Feb-1983 | 26(90) |
| 09-May-2009 | Ugo Monye | Harlequins | England | 13-Apr-1983 | 26(27) |
| 09-May-2009 | Tom Williams | Harlequins | England | 12-Oct-1983 | 25(210) |
| 16-May-2009 | Delon Armitage | London Irish | England | 15-Dec-1983 | 25(153) |
| 16-May-2009 | Julian Dupuy | Leicester Tigers | France | 19-Dec-1983 | 25(149) |
| 09-May-2009 | Will Skinner | Harlequins | England | 08-Feb-1984 | 25(91) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).