| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 20-Sep-2008 |  Peter Homan | Middlesbrough | England | 25-Jul-1991 | 17(58) |
| 06-Dec-2008 | Carl Kirwan | Middlesbrough | England | 21-Mar-1991 | 17(261) |
| 21-Mar-2009 | Max Alderson | Middlesbrough | England | 07-Aug-1990 | 18(227) |
| 01-Nov-2008 | Mohammed Mustafa | Middlesbrough | England | 20-Feb-1990 | 18(255) |
| 25-Oct-2008 | Nick Brocklehurst | Cleckheaton | England | 29-Sep-1989 | 19(26) |
| 13-Sep-2008 | Tom Cruse | Stockport | England | 30-Mar-1989 | 19(167) |
| 11-Oct-2008 | Dominic Parsons | Sheffield Tigers | England | 19-Apr-1989 | 19(175) |
| 13-Sep-2008 | James Cameron | Hull | England | 19-Oct-1988 | 19(329) |
| 31-Jan-2009 | Gavin Charlton | Westoe | England | 03-Feb-1989 | 19(362) |
| 13-Sep-2008 | Mateo Malupo | Hull | Tonga | 07-Jul-1988 | 20(68) |
| 13-Sep-2008 | Aaron Myers | West Hartlepool | England | 30-May-1988 | 20(106) |
| 07-Mar-2009 | Ashely Moeke | Westoe | New Zealand | 09-Mar-1988 | 20(363) |
| 13-Sep-2008 | James Fitzpatrick | Westoe | England | 06-Jul-1987 | 21(70) |
| 13-Sep-2008 | Sione Onesi | Hull | Other | 17-Jun-1985 | 23(88) |
| 13-Sep-2008 |  James Stannard | Morley | England | 08-Aug-1984 | 24(36) |
| 13-Sep-2008 | Alex Piercey | Hull | England | 03-Jul-1984 | 24(72) |
| 13-Sep-2008 | Gareth Morley | Sheffield Tigers | England | 25-May-1984 | 24(111) |
| 13-Sep-2008 | Gareth Kerr | West Hartlepool | Other | 11-Jul-1983 | 25(65) |
| 13-Sep-2008 | Shaun Richardson | Darlington | England | 11-Oct-1982 | 25(338) |
| 20-Sep-2008 | Reuban Harvey | Stockport | New Zealand | 04-Oct-1982 | 25(352) |
| 13-Sep-2008 | Ryan de la Harpe | West Park St Helens | Namibia | 02-Sep-1982 | 26(12) |
| 27-Sep-2008 | Latu Makaafi | Hull | Tonga | 17-Aug-1981 | 27(41) |
| 24-Jan-2009 | Zak Hammond | Stockport | England | 09-Nov-1981 | 27(76) |
| 13-Sep-2008 | Iain Dixon | West Hartlepool | England | 04-Apr-1981 | 27(162) |
| 13-Sep-2008 | Kerry Wood | Westoe | England | 30-Jan-1981 | 27(226) |
| 13-Sep-2008 | Rob Devonshire | Hull | England | 13-Jan-1981 | 27(243) |
| 13-Sep-2008 | Paul Vinnicombe | West Hartlepool | England | 03-Dec-1980 | 27(284) |
| 25-Oct-2008 | Iain Robinson | West Hartlepool | Other | 07-Feb-1980 | 28(261) |
| 15-Nov-2008 | Dave McCabe | Sheffield Tigers | Other | 07-Sep-1979 | 29(70) |
| 11-Oct-2008 | Leon Treco | Morley | England | 27-Sep-1978 | 30(15) |
| 13-Sep-2008 | Nigel Douglas | Westoe | England | 25-Nov-1977 | 30(293) |
| 27-Sep-2008 | Guy Hope | Beverley | England | 27-Oct-1976 | 31(336) |
| 13-Sep-2008 | Scott Plevey | Hull | England | 28-Sep-1976 | 31(350) |
| 27-Sep-2008 | Michael Wood | Hull | England | 15-Jul-1976 | 32(74) |
| 13-Sep-2008 | Chris Murphy | Hull | England | 02-Feb-1976 | 32(224) |
| 13-Sep-2008 | Rob Hitchmough | West Park St Helens | England | 17-Dec-1975 | 32(271) |
| 08-Nov-2008 | Chris Stewart | West Hartlepool | Other | 13-Dec-1974 | 33(331) |
| 13-Sep-2008 | Ben Wade | Cleckheaton | England | 11-Sep-1974 | 34(3) |
| 13-Sep-2008 | Dan Cook | Hull | England | 10-Sep-1974 | 34(4) |
| 04-Oct-2008 | Scott Benton | Morley | England | 08-Sep-1974 | 34(27) |
| 13-Sep-2008 | Jamie Connolly | West Hartlepool | * | 06-Feb-1973 | 35(220) |
| 13-Sep-2008 | Maea David | Hull | Other | 27-Feb-1972 | 36(199) |
| 13-Sep-2008 | Njike Tchakoute | Birkenhead Park | England | 23-Oct-1971 | 36(326) |
| 13-Sep-2008 | Harry Toews | Sheffield Tigers | Canada | 07-Aug-1971 | 37(38) |
| 13-Sep-2008 | Mike Burnett | Morley | England | 09-Dec-1970 | 37(279) |
| 13-Sep-2008 | Simon Bunting | Sheffield Tigers | England | 17-Sep-1970 | 37(362) |
| 25-Oct-2008 | Luke Gross | Sheffield Tigers | United States | 21-Nov-1969 | 38(339) |
| 13-Sep-2008 | David Scully | Sheffield Tigers | England | 07-Aug-1965 | 43(38) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).