| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 23-Nov-2008 |  Tom Homer | London Irish | England | 01-Apr-1990 | 18(237) |
| 07-Sep-2008 |  Rory Clegg | Newcastle Falcons | England | 06-Jan-1990 | 18(245) |
| 20-Dec-2008 | Robert Miller | Newcastle Falcons | England | 05-Aug-1989 | 19(137) |
| 14-Nov-2008 |  Carl Fearns | Sale Sharks | England | 25-May-1989 | 19(173) |
| 21-Feb-2009 |  Charles Sharples | Gloucester | England | 17-Aug-1989 | 19(188) |
| 22-Mar-2009 |  Bob Baker | London Wasps | England | 12-Sep-1989 | 19(191) |
| 16-Nov-2008 | David Lewis | Gloucester | England | 29-Apr-1989 | 19(201) |
| 21-Apr-2009 |  Graham Kitchener | Worcester Warriors | England | 29-Sep-1989 | 19(204) |
| 30-Nov-2008 |  Sebastian Stegmann | Harlequins | England | 12-Apr-1989 | 19(232) |
| 30-Nov-2008 |  Luke Eves | Bristol Bears | England | 11-Apr-1989 | 19(233) |
| 31-Jan-2009 | Henry Trinder | Gloucester | England | 14-Apr-1989 | 19(292) |
| 27-Dec-2008 | Michael Young | Newcastle Falcons | England | 31-Dec-1988 | 19(361) |
| 22-Feb-2009 |  Courtney Lawes | Northampton Saints | England | 23-Feb-1989 | 19(364) |
| 06-Sep-2008 | Alex Corbisiero | London Irish | England | 30-Aug-1988 | 20(7) |
| 12-Sep-2008 |  Andy Saull | Saracens | England | 27-Aug-1988 | 20(16) |
| 14-Feb-2009 | Olly Marchon | Saracens | England | 31-Oct-1988 | 20(106) |
| 22-Nov-2008 | Joe Gray | Northampton Saints | England | 05-Aug-1988 | 20(109) |
| 07-Sep-2008 | Miles Benjamin | Worcester Warriors | England | 11-May-1988 | 20(119) |
| 22-Mar-2009 | William Cliff | Sale Sharks | England | 17-Oct-1988 | 20(156) |
| 02-Oct-2008 | Charles Fellows | Worcester Warriors | England | 26-Apr-1988 | 20(159) |
| 07-Sep-2008 | Alex Tait | Newcastle Falcons | England | 18-Mar-1988 | 20(173) |
| 26-Dec-2008 | Joe Simpson | London Wasps | England | 05-Jul-1988 | 20(174) |
| 16-Nov-2008 | Alex Goode | Saracens | England | 07-May-1988 | 20(193) |
| 16-Nov-2008 | Noah Cato | Saracens | England | 31-Mar-1988 | 20(230) |
| 06-Sep-2008 | Jordan Turner-Hall | Harlequins | England | 05-Jan-1988 | 20(245) |
| 26-Dec-2008 | Hugo Ellis | London Wasps | England | 04-Feb-1988 | 20(326) |
| 01-Oct-2008 | Danny Cipriani | London Wasps | England | 02-Nov-1987 | 20(334) |
| 01-Apr-2009 | Greg King | Worcester Warriors | England | 24-Apr-1988 | 20(342) |
| 06-Sep-2008 | Dominic Waldouck | London Wasps | England | 26-Sep-1987 | 20(346) |
| 11-Jan-2009 | Mathew Turner | Bristol Bears | England | 18-Jan-1988 | 20(359) |
| 20-Sep-2008 |  Jack Cuthbert | Bath | Scotland | 03-Sep-1987 | 21(18) |
| 01-Apr-2009 | Jack Gilding | Worcester Warriors | England | 10-Mar-1988 | 21(22) |
| 07-Sep-2008 | Tom Arscott | Bristol Bears | England | 25-Jul-1987 | 21(45) |
| 16-Nov-2008 | Lachlan Mitchell | London Wasps | Australia | 30-Sep-1987 | 21(48) |
| 01-Apr-2009 | Jacob Abbott | Worcester Warriors | England | 04-Feb-1988 | 21(57) |
| 31-Jan-2009 | Joe Carlisle | Worcester Warriors | England | 04-Dec-1987 | 21(59) |
| 28-Feb-2009 | Alex Grove | Worcester Warriors | Scotland | 30-Nov-1987 | 21(91) |
| 26-Sep-2008 | Tim Visser | Newcastle Falcons | Scotland | 29-May-1987 | 21(121) |
| 26-Sep-2008 |  Dan Cole | Leicester Tigers | England | 09-May-1987 | 21(141) |
| 23-Nov-2008 | David Tait | Sale Sharks | England | 05-Jul-1987 | 21(142) |
| 14-Sep-2008 | Chris Pennell | Worcester Warriors | England | 26-Apr-1987 | 21(142) |
| 31-Jan-2009 | Adam Thompstone | London Irish | England | 04-Sep-1987 | 21(150) |
| 07-Sep-2008 | Chris Ashton | Northampton Saints | England | 29-Mar-1987 | 21(163) |
| 03-Jan-2009 | Tom Mercey | Saracens | England | 15-Jun-1987 | 21(203) |
| 07-Sep-2008 | Mark Sorenson | Newcastle Falcons | New Zealand | 12-Feb-1987 | 21(208) |
| 25-Apr-2009 | James Phillips | Bristol Bears | England | 27-Sep-1987 | 21(211) |
| 07-Sep-2008 | David Doherty | Sale Sharks | England | 28-Jan-1987 | 21(223) |
| 23-Nov-2008 | Marc Jones | Sale Sharks | Wales | 03-Apr-1987 | 21(235) |
| 14-Mar-2009 | Charlie Beech | London Wasps | England | 21-Jul-1987 | 21(237) |
| 16-Nov-2008 | Jack Forster | Gloucester | England | 19-Mar-1987 | 21(243) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).