| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 25-Apr-2009 |  Mike Catt | London Irish | England | 17-Sep-1971 | 37(221) |
| 07-Mar-2009 |  Tom Smith | Northampton Saints | Scotland | 31-Oct-1971 | 37(128) |
| 13-Mar-2009 |  Mark Regan | Bristol Bears | England | 28-Jan-1972 | 37(45) |
| 08-Mar-2009 |  Stuart Turner | Sale Sharks | England | 22-Apr-1972 | 36(320) |
| 25-Apr-2009 | Darren Crompton | Bristol Bears | England | 12-Sep-1972 | 36(225) |
| 13-Mar-2009 |  Mefin Davies | Leicester Tigers | Wales | 02-Sep-1972 | 36(192) |
| 22-Nov-2008 |  Danny Grewcock | Bath | England | 07-Nov-1972 | 36(15) |
| 25-Apr-2009 | Julian White | Leicester Tigers | England | 14-May-1973 | 35(347) |
| 25-Apr-2009 |  Tani Fuga | Harlequins | Samoa | 14-Jul-1973 | 35(286) |
| 23-Nov-2008 |  Raphael Ibanez | London Wasps | France | 17-Feb-1973 | 35(280) |
| 04-Apr-2009 |  Justin Marshall | Saracens | New Zealand | 05-Aug-1973 | 35(243) |
| 25-Apr-2009 | Simon Shaw | London Wasps | England | 01-Sep-1973 | 35(237) |
| 26-Sep-2008 |  Joe McDonnell | Newcastle Falcons | New Zealand | 01-Mar-1973 | 35(210) |
| 27-Mar-2009 | Martin Corry | Leicester Tigers | England | 12-Oct-1973 | 35(167) |
| 21-Mar-2009 | Cobus Visagie | Saracens | South Africa | 31-Oct-1973 | 35(142) |
| 19-Apr-2009 | Kris Chesney | Saracens | England | 02-Mar-1974 | 35(49) |
| 25-Apr-2009 | Serge Betsen | London Wasps | France | 25-Mar-1974 | 35(32) |
| 25-Apr-2009 | Justin Harrison | Bath | Australia | 20-Apr-1974 | 35(6) |
| 07-Mar-2009 |  Tevita Taumoepeau | Worcester Warriors | Tonga | 16-May-1974 | 34(296) |
| 17-Apr-2009 | Andy Gomersall | Harlequins | England | 24-Jul-1974 | 34(268) |
| 25-Apr-2009 | Sebastien Bruno | Sale Sharks | France | 26-Aug-1974 | 34(243) |
| 25-Apr-2009 | Darren Morris | Worcester Warriors | Wales | 24-Sep-1974 | 34(214) |
| 25-Apr-2009 | Duncan Bell | Bath | England | 01-Oct-1974 | 34(207) |
| 04-Apr-2009 |  Olivier Azam | Gloucester | France | 21-Oct-1974 | 34(166) |
| 25-Apr-2009 | Ignacio Fernandez Lobbe | Northampton Saints | Argentina | 20-Nov-1974 | 34(157) |
| 04-Apr-2009 | Brent Cockbain | Sale Sharks | Wales | 15-Nov-1974 | 34(141) |
| 13-Sep-2008 | Kevin Maggs | Bristol Bears | Ireland | 03-Jun-1974 | 34(103) |
| 25-Apr-2009 | Andy Farrell | Saracens | England | 30-May-1975 | 33(331) |
| 25-Apr-2009 | Barry Stewart | Northampton Saints | Scotland | 03-Jun-1975 | 33(327) |
| 30-Sep-2008 | Andy Perry | Newcastle Falcons | England | 28-Dec-1974 | 33(277) |
| 25-Apr-2009 | Russell Winter | Newcastle Falcons | South Africa | 17-Aug-1975 | 33(252) |
| 25-Apr-2009 | Chris Fortey | Worcester Warriors | England | 25-Aug-1975 | 33(244) |
| 25-Apr-2009 | Oriol Ripol | Sale Sharks | Spain | 06-Sep-1975 | 33(232) |
| 05-Apr-2009 | Mark Robinson | London Wasps | New Zealand | 21-Aug-1975 | 33(228) |
| 22-Mar-2009 | Chris Latham | Worcester Warriors | Australia | 08-Sep-1975 | 33(196) |
| 25-Apr-2009 | Carlos Spencer | Gloucester | New Zealand | 14-Oct-1975 | 33(194) |
| 25-Apr-2009 | Glen Jackson | Saracens | New Zealand | 23-Oct-1975 | 33(185) |
| 27-Dec-2008 | Mosese Raulini | Saracens | Fiji | 27-Jun-1975 | 33(184) |
| 13-Sep-2008 | Andrew Blowers | Bristol Bears | New Zealand | 27-Mar-1975 | 33(171) |
| 25-Apr-2009 | Ben Kay | Leicester Tigers | England | 14-Dec-1975 | 33(133) |
| 07-Mar-2009 | Nathan Budgett | Bristol Bears | Wales | 02-Nov-1975 | 33(126) |
| 22-Mar-2009 | Roy Winters | Bristol Bears | England | 13-Dec-1975 | 33(100) |
| 25-Apr-2009 | Loki Crichton | Worcester Warriors | Samoa | 14-Mar-1976 | 33(42) |
| 25-Apr-2009 | Philip Vickery | London Wasps | England | 14-Mar-1976 | 33(42) |
| 22-Mar-2009 | Barry Everitt | Northampton Saints | Ireland | 09-Mar-1976 | 33(13) |
| 18-Apr-2009 | Bruce Reihana | Northampton Saints | New Zealand | 06-Apr-1976 | 33(12) |
| 21-Apr-2009 | Carlos Nieto | Gloucester | Italy | 28-Apr-1976 | 32(358) |
| 01-Apr-2009 | Craig Gillies | Worcester Warriors | England | 06-May-1976 | 32(330) |
| 19-Apr-2009 | George Chuter | Leicester Tigers | England | 09-Jul-1976 | 32(284) |
| 21-Nov-2008 | Graeme Beveridge | Bristol Bears | Scotland | 17-Feb-1976 | 32(278) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).