| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 18-May-2008 |  Danny Cipriani | London Wasps | England | 02-Nov-1987 | 20(198) |
| 31-May-2008 | Dominic Waldouck | London Wasps | England | 26-Sep-1987 | 20(248) |
| 18-May-2008 |  Matt Banahan | Bath | England | 30-Dec-1986 | 21(140) |
| 18-May-2008 |  Anthony Allen | Gloucester | England | 01-Sep-1986 | 21(260) |
| 18-May-2008 |  Jordan Crane | Leicester Tigers | England | 03-Jun-1986 | 21(350) |
| 18-May-2008 | Ryan Lamb | Gloucester | England | 18-May-1986 | 22(1) |
| 18-May-2008 | Tom Varndell | Leicester Tigers | England | 16-Aug-1985 | 22(276) |
| 18-May-2008 | James Haskell | London Wasps | England | 02-Apr-1985 | 23(46) |
| 18-May-2008 | Tom Rees | London Wasps | England | 11-Sep-1984 | 23(249) |
| 18-May-2008 | Akapusi Qera | Gloucester | Fiji | 24-Apr-1984 | 24(24) |
| 18-May-2008 | Jonathan Faamatuainu | Bath | Samoa | 29-Dec-1983 | 24(141) |
| 18-May-2008 | Alasdair Strokosch | Gloucester | Scotland | 21-Feb-1983 | 25(87) |
| 18-May-2008 | Nick Wood | Gloucester | England | 09-Jan-1983 | 25(130) |
| 18-May-2008 | Gareth Delve | Gloucester | Wales | 30-Dec-1982 | 25(140) |
| 18-May-2008 | Matthew Stevens | Bath | England | 01-Oct-1982 | 25(230) |
| 18-May-2008 | Dan Hipkiss | Leicester Tigers | England | 04-Jun-1982 | 25(349) |
| 18-May-2008 | James Simpson-Daniel | Gloucester | England | 30-May-1982 | 25(354) |
| 18-May-2008 | Harry Ellis | Leicester Tigers | England | 17-May-1982 | 26(2) |
| 18-May-2008 | Michael Claassens | Bath | South Africa | 29-Jan-1982 | 26(110) |
| 18-May-2008 | Olly Barkley | Bath | England | 28-Nov-1981 | 26(172) |
| 18-May-2008 | Alex Crockett | Bath | England | 20-Nov-1981 | 26(180) |
| 18-May-2008 | Andrew Higgins | Bath | England | 13-Jul-1981 | 26(310) |
| 18-May-2008 | Pat Barnard | London Wasps | England | 03-Jul-1981 | 26(320) |
| 18-May-2008 | Rory Lawson | Gloucester | Scotland | 12-Mar-1981 | 27(67) |
| 18-May-2008 | Alesana Tuilagi | Leicester Tigers | Samoa | 24-Feb-1981 | 27(83) |
| 18-May-2008 | Andrew Titterell | Gloucester | England | 10-Jan-1981 | 27(128) |
| 18-May-2008 | Tom Voyce | London Wasps | England | 05-Jan-1981 | 27(133) |
| 18-May-2008 | Aaron Mauger | Leicester Tigers | New Zealand | 29-Nov-1980 | 27(170) |
| 18-May-2008 | Eion Redden | London Wasps | Ireland | 20-Nov-1980 | 27(179) |
| 18-May-2008 | Boris Stankovich | Leicester Tigers | England | 01-Sep-1980 | 27(259) |
| 18-May-2008 | Marco Bortolami | Gloucester | Italy | 12-Jun-1980 | 27(340) |
| 18-May-2008 | Joe Ward | London Wasps | England | 03-Jun-1980 | 27(349) |
| 18-May-2008 | Andy Goode | Leicester Tigers | England | 03-Apr-1980 | 28(45) |
| 18-May-2008 | Ben Herring | Leicester Tigers | New Zealand | 14-Mar-1980 | 28(65) |
| 18-May-2008 | Riki Flutey | London Wasps | England | 10-Feb-1980 | 28(98) |
| 18-May-2008 | Michael Lipman | Bath | England | 16-Jan-1980 | 28(123) |
| 18-May-2008 | Paul Sackey | London Wasps | England | 08-Nov-1979 | 28(192) |
| 18-May-2008 | Steve Borthwick | Bath | England | 12-Oct-1979 | 28(219) |
| 18-May-2008 | Butch James | Bath | South Africa | 08-Jul-1979 | 28(315) |
| 18-May-2008 | Alex Brown | Gloucester | England | 17-May-1979 | 29(2) |
| 18-May-2008 | Marco Wentzel | Leicester Tigers | South Africa | 06-May-1979 | 29(13) |
| 18-May-2008 | Lesley Vainikolo | Gloucester | England | 04-May-1979 | 29(15) |
| 18-May-2008 | Tim Payne | London Wasps | England | 29-Apr-1979 | 29(20) |
| 18-May-2008 | Iain Balshaw | Gloucester | England | 18-Apr-1979 | 29(31) |
| 18-May-2008 | Daniel Browne | Bath | Ireland | 13-Apr-1979 | 29(36) |
| 18-May-2008 | Tom Palmer | London Wasps | England | 27-Mar-1979 | 29(53) |
| 18-May-2008 | Shaun Berne | Bath | Australia | 08-Jan-1979 | 29(131) |
| 18-May-2008 | Joe Maddock | Bath | New Zealand | 20-Dec-1978 | 29(150) |
| 18-May-2008 | Willie Walker | Gloucester | New Zealand | 19-May-1978 | 29(365) |
| 18-May-2008 | Geordan Murphy | Leicester Tigers | Ireland | 19-Apr-1978 | 30(30) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).