| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 31-May-2008 |  Lawrence Dallaglio | London Wasps | England | 10-Aug-1972 | 35(295) |
| 31-May-2008 |  Mefin Davies | Leicester Tigers | Wales | 02-Sep-1972 | 35(272) |
| 18-May-2008 |  Danny Grewcock | Bath | England | 07-Nov-1972 | 35(193) |
| 31-May-2008 | Raphael Ibanez | London Wasps | France | 17-Feb-1973 | 35(104) |
| 31-May-2008 | Julian White | Leicester Tigers | England | 14-May-1973 | 35(18) |
| 31-May-2008 | Simon Shaw | London Wasps | England | 01-Sep-1973 | 34(273) |
| 31-May-2008 | Martin Corry | Leicester Tigers | England | 12-Oct-1973 | 34(232) |
| 31-May-2008 | Ben Kay | Leicester Tigers | England | 14-Dec-1975 | 32(169) |
| 31-May-2008 | Philip Vickery | London Wasps | England | 14-Mar-1976 | 32(78) |
| 31-May-2008 | Fraser Waters | London Wasps | England | 31-Mar-1976 | 32(61) |
| 18-May-2008 |  Carlos Nieto | Gloucester | Italy | 28-Apr-1976 | 32(20) |
| 18-May-2008 | David Barnes | Bath | England | 12-Jul-1976 | 31(310) |
| 31-May-2008 | Josh Lewsey | London Wasps | England | 30-Nov-1976 | 31(183) |
| 31-May-2008 | Mark van Gisbergen | London Wasps | England | 30-Jun-1977 | 30(336) |
| 18-May-2008 | Pieter Dixon | Bath | Zimbabwe | 17-Oct-1977 | 30(214) |
| 31-May-2008 | Geordan Murphy | Leicester Tigers | Ireland | 19-Apr-1978 | 30(43) |
| 18-May-2008 | Willie Walker | Gloucester | New Zealand | 19-May-1978 | 29(365) |
| 18-May-2008 | Joe Maddock | Bath | New Zealand | 20-Dec-1978 | 29(150) |
| 18-May-2008 | Shaun Berne | Bath | Australia | 08-Jan-1979 | 29(131) |
| 31-May-2008 | Tom Palmer | London Wasps | England | 27-Mar-1979 | 29(66) |
| 18-May-2008 | Daniel Browne | Bath | Ireland | 13-Apr-1979 | 29(36) |
| 31-May-2008 | Tim Payne | London Wasps | England | 29-Apr-1979 | 29(33) |
| 18-May-2008 | Iain Balshaw | Gloucester | England | 18-Apr-1979 | 29(31) |
| 31-May-2008 | Marco Wentzel | Leicester Tigers | South Africa | 06-May-1979 | 29(26) |
| 18-May-2008 | Lesley Vainikolo | Gloucester | England | 04-May-1979 | 29(15) |
| 18-May-2008 | Alex Brown | Gloucester | England | 17-May-1979 | 29(2) |
| 18-May-2008 | Butch James | Bath | South Africa | 08-Jul-1979 | 28(315) |
| 18-May-2008 | Steve Borthwick | Bath | England | 12-Oct-1979 | 28(219) |
| 31-May-2008 | Paul Sackey | London Wasps | England | 08-Nov-1979 | 28(205) |
| 18-May-2008 | Michael Lipman | Bath | England | 16-Jan-1980 | 28(123) |
| 31-May-2008 | Riki Flutey | London Wasps | England | 10-Feb-1980 | 28(111) |
| 31-May-2008 | Ben Herring | Leicester Tigers | New Zealand | 14-Mar-1980 | 28(78) |
| 31-May-2008 | Andy Goode | Leicester Tigers | England | 03-Apr-1980 | 28(58) |
| 18-May-2008 | Joe Ward | London Wasps | England | 03-Jun-1980 | 27(349) |
| 18-May-2008 | Marco Bortolami | Gloucester | Italy | 12-Jun-1980 | 27(340) |
| 31-May-2008 | Boris Stankovich | Leicester Tigers | England | 01-Sep-1980 | 27(272) |
| 31-May-2008 | Eion Redden | London Wasps | Ireland | 20-Nov-1980 | 27(192) |
| 31-May-2008 | Aaron Mauger | Leicester Tigers | New Zealand | 29-Nov-1980 | 27(183) |
| 18-May-2008 | Tom Voyce | London Wasps | England | 05-Jan-1981 | 27(133) |
| 18-May-2008 | Andrew Titterell | Gloucester | England | 10-Jan-1981 | 27(128) |
| 31-May-2008 | Alesana Tuilagi | Leicester Tigers | Samoa | 24-Feb-1981 | 27(96) |
| 18-May-2008 | Rory Lawson | Gloucester | Scotland | 12-Mar-1981 | 27(67) |
| 18-May-2008 | Pat Barnard | London Wasps | England | 03-Jul-1981 | 26(320) |
| 18-May-2008 | Andrew Higgins | Bath | England | 13-Jul-1981 | 26(310) |
| 18-May-2008 | Alex Crockett | Bath | England | 20-Nov-1981 | 26(180) |
| 18-May-2008 | Olly Barkley | Bath | England | 28-Nov-1981 | 26(172) |
| 18-May-2008 | Michael Claassens | Bath | South Africa | 29-Jan-1982 | 26(110) |
| 31-May-2008 | Harry Ellis | Leicester Tigers | England | 17-May-1982 | 26(15) |
| 31-May-2008 | Dan Hipkiss | Leicester Tigers | England | 04-Jun-1982 | 25(362) |
| 18-May-2008 | James Simpson-Daniel | Gloucester | England | 30-May-1982 | 25(354) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).