| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 04-Nov-2000 |  Ollie Smith | Leicester Tigers | England | 14-Aug-1982 | 18(83) |
| 07-Oct-2000 |  Pat Sykes | Westcombe Park | England | 12-Feb-1982 | 18(238) |
| 11-Nov-2000 |  Chris Hyndman | Northampton Saints | England | 06-Mar-1982 | 18(251) |
| 07-Oct-2000 |  Russell Flynn | Preston Grasshoppers | England | 10-Aug-1981 | 19(58) |
| 21-Oct-2000 |  John Rudd | Bedford Blues | England | 26-May-1981 | 19(148) |
| 21-Oct-2000 | Ross Winney | Wakefield | England | 25-May-1981 | 19(149) |
| 07-Oct-2000 |  Gareth Roberts | Sedgley Park | England | 30-Apr-1981 | 19(160) |
| 07-Oct-2000 |  Jody Peacock | Nuneaton | England | 22-Mar-1981 | 19(199) |
| 11-Nov-2000 | Mark Soden | Northampton Saints | England | 10-Apr-1981 | 19(215) |
| 07-Oct-2000 | John Carter | Nuneaton | England | 16-Feb-1981 | 19(233) |
| 07-Oct-2000 |  Paul Thirlby | Redruth | England | 30-Jan-1981 | 19(250) |
| 04-Nov-2000 |  Gerald Arasa | Saracens | * | 21-Dec-1980 | 19(318) |
| 07-Oct-2000 | Ryan Banks | Nuneaton | England | 22-Sep-1980 | 20(15) |
| 07-Oct-2000 | David Wiseman | Fylde | England | 20-Sep-1980 | 20(17) |
| 07-Oct-2000 | Elijah Sobanjo | Sedgley Park | England | 18-Sep-1980 | 20(19) |
| 10-Dec-2000 |  Charlie Hodgson | Sale Sharks | England | 12-Nov-1980 | 20(28) |
| 04-Nov-2000 | Louis Deacon | Leicester Tigers | England | 07-Oct-1980 | 20(28) |
| 07-Oct-2000 |  Ben Murphy | Nottingham | England | 03-Sep-1980 | 20(34) |
| 04-Nov-2000 |  Michael Stephenson | Newcastle Falcons | England | 20-Sep-1980 | 20(45) |
| 07-Oct-2000 |  Mike Blakeburn | Camberley | England | 15-Aug-1980 | 20(53) |
| 21-Oct-2000 |  Richard Mahony | London Welsh | England | 23-Aug-1980 | 20(59) |
| 07-Oct-2000 | Paul Arnold | Sedgley Park | England | 30-Jul-1980 | 20(69) |
| 04-Nov-2000 | James Grindal | Leicester Tigers | England | 18-Aug-1980 | 20(78) |
| 07-Oct-2000 | Matt Vick | Westcombe Park | England | 15-Jul-1980 | 20(84) |
| 23-Sep-2000 | Rob Brookshaw | Whitchurch | England | 17-Jun-1980 | 20(98) |
| 07-Oct-2000 |  Andrew Murray | Tynedale | England | 29-Jun-1980 | 20(100) |
| 07-Oct-2000 | Ben Blackwall | Nottingham | England | 07-Jun-1980 | 20(122) |
| 07-Oct-2000 |  Adam Holloway | Reading | England | 05-Jun-1980 | 20(124) |
| 21-Oct-2000 |  Graham Barr | Esher | Scotland | 31-May-1980 | 20(143) |
| 07-Oct-2000 |  James Ball | Morley | England | 17-Apr-1980 | 20(173) |
| 11-Nov-2000 |  Ben Gollings | Harlequins | England | 12-May-1980 | 20(183) |
| 23-Sep-2000 | Andrew Roberts | Tynedale | England | 08-Mar-1980 | 20(199) |
| 21-Oct-2000 | Phil Murphy | Leeds Tykes | England | 01-Apr-1980 | 20(203) |
| 04-Nov-2000 | Jon Dawson | Harlequins | England | 12-Apr-1980 | 20(206) |
| 04-Nov-2000 |  Gavin Cattle | Birmingham Solihull | England | 05-Apr-1980 | 20(213) |
| 04-Nov-2000 | Andy Goode | Leicester Tigers | England | 03-Apr-1980 | 20(215) |
| 07-Oct-2000 | Richard Kenyon | Fylde | England | 23-Feb-1980 | 20(227) |
| 07-Oct-2000 |  Charles Vine | Lydney | England | 28-Jan-1980 | 20(253) |
| 04-Nov-2000 |  Neil Hallett | Rosslyn Park | England | 18-Feb-1980 | 20(260) |
| 04-Nov-2000 | David Flatman | Saracens | England | 21-Jan-1980 | 20(288) |
| 09-Dec-2000 | Joe Shaw | Northampton Saints | England | 20-Feb-1980 | 20(293) |
| 04-Nov-2000 | Adam M Jones | Harlequins | Wales | 12-Jan-1980 | 20(297) |
| 23-Sep-2000 |  Michael Jones | Liverpool St Helens | England | 10-Nov-1979 | 20(318) |
| 07-Oct-2000 | Andrew Grierson | Reading | * | 23-Nov-1979 | 20(319) |
| 07-Oct-2000 | Gareth Taylor | Nottingham | England | 19-Nov-1979 | 20(323) |
| 04-Nov-2000 | Adam Balding | Leicester Tigers | England | 07-Dec-1979 | 20(333) |
| 07-Oct-2000 | Matt Senior | Reading | * | 25-Oct-1979 | 20(348) |
| 12-Nov-2000 | Tom Shanklin | Saracens | Wales | 24-Nov-1979 | 20(354) |
| 10-Dec-2000 | Gareth Maclure | Newcastle Falcons | * | 17-Dec-1979 | 20(359) |
| 11-Nov-2000 |  Paul Sackey | London Irish | England | 08-Nov-1979 | 21(4) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).