| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 03-Jul-2020 |  Gus Bell | New South Wales | Australia | 04-Oct-2000 | 19(272) |
| 03-Jul-2020 | Mark Nawaqanitawase | New South Wales | Australia | 11-Sep-2000 | 19(295) |
| 03-Jul-2020 | Will Harris | New South Wales | Australia | 08-Jun-2000 | 20(25) |
| 03-Jul-2020 | Joey Walton | New South Wales | Australia | 27-May-2000 | 20(37) |
| 11-Jul-2020 | Byron Ralston | Western Force | Australia | 03-May-2000 | 20(69) |
| 15-Aug-2020 | Jordan Petaia | Queensland Reds | Australia | 14-Mar-2000 | 20(154) |
| 04-Jul-2020 | Noah Lolesio | Brumbies | Australia | 18-Dec-1999 | 20(199) |
| 03-Jul-2020 | Harry Wilson | Queensland Reds | Australia | 22-Nov-1999 | 20(224) |
| 24-Jul-2020 | Trevor Hosea | Melbourne Rebels | Australia | 24-Nov-1999 | 20(243) |
| 25-Jul-2020 | Nick Frost | Brumbies | Australia | 10-Oct-1999 | 20(289) |
| 03-Jul-2020 | Will Harrison | New South Wales | Australia | 30-Jul-1999 | 20(339) |
| 31-Jul-2020 | Esei Ha'angana | Melbourne Rebels | Australia | 21-Apr-1999 | 21(102) |
| 03-Jul-2020 | Fraser McReight | Queensland Reds | Australia | 19-Feb-1999 | 21(135) |
| 04-Jul-2020 | Josh Kemeny | Melbourne Rebels | Australia | 29-Nov-1998 | 21(218) |
| 03-Jul-2020 | Tata McDermott | Queensland Reds | Australia | 18-Sep-1998 | 21(289) |
| 03-Jul-2020 | Harry Hoopert | Queensland Reds | Australia | 16-Sep-1998 | 21(291) |
| 04-Jul-2020 | Robert Valetini | Brumbies | Australia | 03-Sep-1998 | 21(305) |
| 25-Jul-2020 | Bayley Kuenzle | Brumbies | Australia | 18-Jun-1998 | 22(38) |
| 03-Jul-2020 | James Ramm | New South Wales | Australia | 30-Apr-1998 | 22(65) |
| 03-Jul-2020 | Okoumene Paisami | Queensland Reds | Australia | 10-Apr-1998 | 22(85) |
| 04-Jul-2020 | Mackenzie Hansen | Brumbies | Ireland | 27-Mar-1998 | 22(100) |
| 03-Jul-2020 | Angus Blyth | Queensland Reds | Australia | 04-Mar-1998 | 22(122) |
| 03-Jul-2020 | Hamish Stewart | Queensland Reds | Australia | 03-Mar-1998 | 22(123) |
| 22-Aug-2020 | Ryan Lonergan | Brumbies | Australia | 06-Apr-1998 | 22(139) |
| 03-Jul-2020 | Liam Wright | Queensland Reds | Australia | 06-Nov-1997 | 22(240) |
| 11-Jul-2020 | Angus Wagner | Western Force | Australia | 30-Oct-1997 | 22(255) |
| 04-Jul-2020 | Tom Wright | Brumbies | Australia | 21-Jul-1997 | 22(349) |
| 04-Jul-2020 | Darcy Swain | Brumbies | Australia | 05-Jul-1997 | 22(365) |
| 11-Jul-2020 | Nicolas Jooste | Western Force | Australia | 07-Jul-1997 | 23(4) |
| 17-Jul-2020 | Angus Scott-Young | Queensland Reds | Australia | 23-Apr-1997 | 23(85) |
| 18-Jul-2020 | Tom Horton | New South Wales | Australia | 18-Apr-1997 | 23(91) |
| 03-Jul-2020 | Harry Johnson-Holmes | New South Wales | Australia | 02-Mar-1997 | 23(123) |
| 04-Jul-2020 | Pone Fa'amuasili | Melbourne Rebels | Australia | 26-Feb-1997 | 23(128) |
| 03-Jul-2020 | Jack Maddocks | New South Wales | Australia | 05-Feb-1997 | 23(148) |
| 04-Jul-2020 | Jordan Uelese | Melbourne Rebels | Australia | 24-Jan-1997 | 23(161) |
| 03-Jul-2020 | Lachlan Swinton | New South Wales | Australia | 16-Jan-1997 | 23(168) |
| 11-Jul-2020 | Jack McGregor | Western Force | Australia | 10-Jan-1997 | 23(182) |
| 14-Aug-2020 | Jake Strachan | Western Force | Australia | 25-Dec-1996 | 23(232) |
| 10-Jul-2020 | Campbell Magnay | Melbourne Rebels | Australia | 10-Nov-1996 | 23(242) |
| 03-Jul-2020 | Lukhan Salakaia-Loto | Queensland Reds | Australia | 19-Sep-1996 | 23(287) |
| 11-Jul-2020 | Tevin Ferris | Western Force | Australia | 24-Jul-1996 | 23(352) |
| 03-Jul-2020 | Taniela Tupou | Queensland Reds | Tonga | 10-May-1996 | 24(54) |
| 03-Jul-2020 | Brandon Paenga-Amosa | Queensland Reds | Australia | 25-Dec-1995 | 24(191) |
| 07-Aug-2020 | Brad Wilkin | Melbourne Rebels | Australia | 08-Dec-1995 | 24(243) |
| 04-Jul-2020 | Andrew Kellaway | Melbourne Rebels | Australia | 12-Oct-1995 | 24(266) |
| 29-Aug-2020 | Tepai Moeroa | New South Wales | Cook Islands | 02-Oct-1995 | 24(332) |
| 31-Jul-2020 | Jermaine Ainsley | Melbourne Rebels | Australia | 08-Aug-1995 | 24(358) |
| 04-Jul-2020 | Irae Simone | Brumbies | Australia | 10-Jul-1995 | 24(360) |
| 04-Jul-2020 | Matt Gibbon | Melbourne Rebels | Australia | 03-Jun-1995 | 25(32) |
| 03-Jul-2020 | Mitch Short | New South Wales | Australia | 25-May-1995 | 25(40) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).