| MD | Players | Nationality | DOB | A(D) |
|---|---|---|---|---|
| 13-Jan-2001 | Tom Voyce | England | 05-Jan-1981 | 20(8) |
| 07-Oct-2000 | Steve Borthwick | England | 12-Oct-1979 | 20(361) |
| 07-Oct-2000 | Gareth Cooper | Wales | 07-May-1979 | 21(154) |
| 07-Oct-2000 | Iain Balshaw | England | 18-Apr-1979 | 21(173) |
| 07-Oct-2000 | Mike Tindall | England | 18-Oct-1978 | 21(355) |
| 13-Jan-2001 | Shaun Berne | Australia | 08-Jan-1979 | 22(6) |
| 07-Oct-2000 | Gavin Thomas | Wales | 22-Oct-1977 | 22(351) |
| 07-Oct-2000 | Matt Perry | England | 27-Jan-1977 | 23(253) |
| 21-Oct-2000 | Chris Horsman | Wales | 02-Feb-1977 | 23(261) |
| 07-Oct-2000 | David Barnes | England | 12-Jul-1976 | 24(87) |
| 28-Oct-2000 | Simon Emms | Wales | 27-Jan-1975 | 25(275) |
| 21-Oct-2000 | Kevin Maggs | Ireland | 03-Jun-1974 | 26(141) |
| 07-Oct-2000 | Mark Regan | England | 28-Jan-1972 | 28(253) |
| 21-Oct-2000 | Angus Gardiner | New Zealand | 06-Oct-1971 | 29(16) |
| 28-Oct-2000 | Mike Catt | England | 17-Sep-1971 | 29(42) |
| 07-Oct-2000 | Adebayo Adebayo | England | 30-Nov-1970 | 29(312) |
| 07-Oct-2000 | Dan Lyle | United States | 28-Sep-1970 | 30(10) |
| 07-Oct-2000 | John Mallett | England | 28-May-1970 | 30(133) |
| 07-Oct-2000 | Phil De Glanville | England | 01-Oct-1968 | 32(6) |
| 07-Oct-2000 | Martin Haag | England | 28-Jul-1968 | 32(71) |
| 07-Oct-2000 | Ben Clarke | England | 15-Apr-1968 | 32(175) |
| 07-Oct-2000 | Jon Preston | New Zealand | 15-Nov-1967 | 32(327) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).