| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 22-Oct-2000 |  Clement Poitrenaud | Toulouse | France | 20-May-1982 | 18(156) |
| 20-Jan-2001 |  Ollie Smith | Leicester Tigers | England | 14-Aug-1982 | 18(160) |
| 28-Oct-2000 |  Gavin Henson | Swansea | Wales | 01-Feb-1982 | 18(270) |
| 21-Mar-2001 |  James Simpson-Daniel | Gloucester | England | 30-May-1982 | 18(296) |
| 07-Oct-2000 |  Ian Vass | Northampton Saints | England | 17-Aug-1981 | 19(51) |
| 07-Oct-2000 | Andrea Masi | LAquila | Italy | 30-Mar-1981 | 19(191) |
| 07-Oct-2000 | Simon Webster | Northampton Saints | Scotland | 08-Mar-1981 | 19(213) |
| 21-Oct-2000 | Vincent Clement | Colomiers | France | 14-Mar-1981 | 19(221) |
| 21-Jan-2001 |  Alex Page | London Wasps | England | 29-May-1981 | 19(237) |
| 07-Oct-2000 | Michael Owen | Pontypridd | Wales | 07-Nov-1980 | 19(334) |
| 14-Oct-2000 | Mathieu Larrouy | Pau | France | 11-Oct-1980 | 20(3) |
| 13-Jan-2001 | Tom Voyce | Bath | England | 05-Jan-1981 | 20(8) |
| 07-Oct-2000 | Andrew Farley | LAquila | Ireland | 21-Aug-1980 | 20(47) |
| 07-Oct-2000 |  Raphael Poulain | Stade Francais | France | 16-Aug-1980 | 20(52) |
| 21-Jan-2001 | Matthew Leek | London Wasps | England | 25-Oct-1980 | 20(88) |
| 13-Jan-2001 | Louis Deacon | Leicester Tigers | England | 07-Oct-1980 | 20(98) |
| 07-Oct-2000 | Andy Goode | Leicester Tigers | England | 03-Apr-1980 | 20(187) |
| 14-Oct-2000 |  Jamie Robinson | Cardiff Blues | Wales | 07-Apr-1980 | 20(190) |
| 07-Oct-2000 | Jonathan Steel | Glasgow Warriors | Scotland | 14-Mar-1980 | 20(207) |
| 06-Oct-2000 | Rhys Williams | Cardiff Blues | Wales | 23-Feb-1980 | 20(226) |
| 07-Oct-2000 | Imanol Harinordoquy | Pau | France | 20-Feb-1980 | 20(230) |
| 06-Oct-2000 |  Gordon D'Arcy | Leinster | Ireland | 10-Feb-1980 | 20(239) |
| 07-Oct-2000 | Federico Angeloni | LAquila | Italy | 31-Jan-1980 | 20(250) |
| 07-Oct-2000 |  David Flatman | Saracens | England | 21-Jan-1980 | 20(260) |
| 22-Oct-2000 | Sylvain Guilhem | Pau | France | 01-Feb-1980 | 20(264) |
| 13-Jan-2001 | Andrew Rennick | Northampton Saints | Scotland | 18-Mar-1980 | 20(301) |
| 15-Oct-2000 | Adam Balding | Leicester Tigers | England | 07-Dec-1979 | 20(313) |
| 20-Jan-2001 | Richard Johnston | Pontypridd | Wales | 26-Feb-1980 | 20(329) |
| 06-Oct-2000 |  Mark Jones | Scarlets | Wales | 14-Oct-1979 | 20(358) |
| 07-Oct-2000 | Steve Borthwick | Bath | England | 12-Oct-1979 | 20(361) |
| 21-Oct-2000 | Alix Popham | Newport | Wales | 17-Oct-1979 | 21(5) |
| 14-Jan-2001 | Colin Stewart | Glasgow Warriors | Scotland | 06-Jan-1980 | 21(9) |
| 06-Oct-2000 |  Simon Taylor | Edinburgh Rugby | Scotland | 17-Aug-1979 | 21(51) |
| 14-Jan-2001 | Tom Shanklin | Saracens | Wales | 24-Nov-1979 | 21(52) |
| 20-Jan-2001 | Donald McFadyen | Glasgow Warriors | Scotland | 11-Oct-1979 | 21(102) |
| 06-Oct-2000 | Dafydd Jones | Scarlets | Wales | 24-Jun-1979 | 21(105) |
| 14-Jan-2001 | Richard Birkett | London Wasps | England | 01-Oct-1979 | 21(106) |
| 07-Oct-2000 | Robert Sidoli | Pontypridd | Wales | 21-Jun-1979 | 21(109) |
| 07-Oct-2000 | Damien Traille | Pau | France | 12-Jun-1979 | 21(118) |
| 23-Jan-2001 | Scott Mitchell | Newport | Wales | 21-Sep-1979 | 21(125) |
| 07-Oct-2000 | Alexandre Albouy | Castres | France | 26-May-1979 | 21(135) |
| 07-Oct-2000 | Gareth Cooper | Bath | Wales | 07-May-1979 | 21(154) |
| 07-Oct-2000 | Iain Balshaw | Bath | England | 18-Apr-1979 | 21(173) |
| 19-Jan-2001 | Matthias Rolland | Colomiers | France | 02-Jul-1979 | 21(202) |
| 28-Oct-2000 | Cerith Rees | Swansea | Wales | 01-Mar-1979 | 21(242) |
| 06-Oct-2000 | Brian O'Driscoll | Leinster | Ireland | 21-Jan-1979 | 21(259) |
| 06-Oct-2000 | Adam Eustace | Gloucester | England | 09-Jan-1979 | 21(271) |
| 07-Oct-2000 | Jean Bouilhou | Toulouse | France | 07-Dec-1978 | 21(305) |
| 07-Oct-2000 | Ramiro Pez | Roma | Italy | 06-Dec-1978 | 21(306) |
| 07-Oct-2000 | Ben Johnston | Saracens | England | 08-Nov-1978 | 21(334) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).