| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 21-May-2016 |  Zander Fagerson | Glasgow Warriors | Scotland | 19-Jan-1996 | 20(123) |
| 20-May-2016 |  Gary Ringrose | Leinster | Ireland | 26-Jan-1995 | 21(115) |
| 28-May-2016 | Ross Moloney | Leinster | Ireland | 11-May-1994 | 22(18) |
| 21-May-2016 | Jonny Gray | Glasgow Warriors | Scotland | 14-Mar-1994 | 22(69) |
| 21-May-2016 | Ultan Dilane | Connacht | Ireland | 09-Nov-1993 | 22(194) |
| 21-May-2016 | Robbie Henshaw | Connacht | Ireland | 12-Jun-1993 | 22(344) |
| 21-May-2016 | Mark Bennett | Glasgow Warriors | Scotland | 03-Feb-1993 | 23(107) |
| 21-May-2016 | Finn Russell | Glasgow Warriors | Scotland | 23-Sep-1992 | 23(240) |
| 20-May-2016 |  Stuart McCloskey | Ulster | Ireland | 06-Aug-1992 | 23(287) |
| 21-May-2016 | Stuart Hogg | Glasgow Warriors | Scotland | 24-Jun-1992 | 23(331) |
| 21-May-2016 | Jake Heenan | Connacht | New Zealand | 17-Mar-1992 | 24(65) |
| 20-May-2016 | Iain Henderson | Ulster | Ireland | 21-Feb-1992 | 24(89) |
| 21-May-2016 | Kieran Marmion | Connacht | Ireland | 11-Feb-1992 | 24(100) |
| 20-May-2016 | Paddy Jackson | Ulster | Ireland | 05-Jan-1992 | 24(136) |
| 21-May-2016 | Finley Bealham | Connacht | Ireland | 09-Oct-1991 | 24(225) |
| 21-May-2016 | Eoin McKeown | Connacht | Ireland | 23-Jun-1991 | 24(333) |
| 20-May-2016 | Jordi Murphy | Leinster | Ireland | 22-Apr-1991 | 25(29) |
| 20-May-2016 | Michael Kearney | Leinster | Ireland | 29-Mar-1991 | 25(53) |
| 20-May-2016 | Craig Gilroy | Ulster | Ireland | 11-Mar-1991 | 25(71) |
| 20-May-2016 | Luke Marshall | Ulster | Ireland | 03-Mar-1991 | 25(79) |
| 21-May-2016 | Tiernan O'Halloran | Connacht | Ireland | 26-Feb-1991 | 25(85) |
| 21-May-2016 | Niyi Adeolokun | Connacht | Ireland | 03-Nov-1990 | 25(200) |
| 20-May-2016 | Rhys Ruddock | Leinster | Ireland | 13-Oct-1990 | 25(220) |
| 21-May-2016 | Bundee Aki | Connacht | Ireland | 07-Apr-1990 | 26(45) |
| 21-May-2016 | AJ MacGinty | Connacht | United States | 26-Feb-1990 | 26(85) |
| 20-May-2016 | Jack McGrath | Leinster | Ireland | 11-Oct-1989 | 26(222) |
| 21-May-2016 | Peter Horne | Glasgow Warriors | Scotland | 05-Oct-1989 | 26(229) |
| 21-May-2016 | Henry Pyrgos | Glasgow Warriors | Scotland | 09-Jul-1989 | 26(317) |
| 20-May-2016 | David Kearney | Leinster | Ireland | 19-Jun-1989 | 26(336) |
| 21-May-2016 | Fraser Brown | Glasgow Warriors | Scotland | 20-Jun-1989 | 26(336) |
| 21-May-2016 | Ryan Wilson | Glasgow Warriors | Scotland | 18-May-1989 | 27(3) |
| 20-May-2016 | Sean Reidy | Ulster | Ireland | 10-May-1989 | 27(10) |
| 21-May-2016 | Matthew Healy | Connacht | Ireland | 14-Mar-1989 | 27(68) |
| 21-May-2016 | Simone Favaro | Glasgow Warriors | Italy | 07-Nov-1988 | 27(195) |
| 21-May-2016 | Tommy Seymour | Glasgow Warriors | Scotland | 01-Jul-1988 | 27(324) |
| 21-May-2016 | Leone Nakarawa | Glasgow Warriors | Fiji | 02-Apr-1988 | 28(49) |
| 20-May-2016 | Peter Browne | Ulster | England | 25-Oct-1987 | 28(208) |
| 20-May-2016 | Luke Fitzgerald | Leinster | Ireland | 13-Sep-1987 | 28(250) |
| 21-May-2016 | Gordon Reid | Glasgow Warriors | Scotland | 04-Mar-1987 | 29(79) |
| 20-May-2016 | Ben Te'o | Leinster | England | 27-Jan-1987 | 29(114) |
| 21-May-2016 | Joshua Strauss | Glasgow Warriors | Scotland | 23-Oct-1986 | 29(211) |
| 20-May-2016 | Devin Toner | Leinster | Ireland | 29-Jun-1986 | 29(326) |
| 20-May-2016 | Richard Lutton | Ulster | England | 26-Feb-1986 | 30(84) |
| 20-May-2016 | Callum Black | Ulster | England | 25-Feb-1986 | 30(85) |
| 20-May-2016 | Richard Strauss | Leinster | Ireland | 29-Jan-1986 | 30(112) |
| 20-May-2016 | Jared Payne | Ulster | Ireland | 13-Oct-1985 | 30(220) |
| 21-May-2016 | Tom McCartney | Connacht | New Zealand | 06-Sep-1985 | 30(258) |
| 20-May-2016 | Jonathan Sexton | Leinster | Ireland | 11-Jul-1985 | 30(314) |
| 21-May-2016 | Ronan Loughney | Connacht | Ireland | 01-Nov-1984 | 31(202) |
| 20-May-2016 | Andrew Trimble | Ulster | Ireland | 20-Oct-1984 | 31(212) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).