| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 19-Mar-2016 |  Reiko Ioane | Blues | New Zealand | 18-Mar-1997 | 19(1) |
| 27-Feb-2016 | Shaun Stevenson | Chiefs | New Zealand | 14-Nov-1996 | 19(104) |
| 09-Jul-2016 | Sione Tuipulotu | Melbourne Rebels | Australia | 12-Feb-1997 | 19(147) |
| 06-May-2016 | Campbell Magnay | Queensland Reds | Australia | 10-Nov-1996 | 19(177) |
| 01-Jul-2016 | Lukhan Lealaiaulolo-Tui | Queensland Reds | Australia | 19-Sep-1996 | 19(285) |
| 19-Mar-2016 | Ruan Ackermann | Lions | South Africa | 29-Dec-1995 | 20(81) |
| 09-Apr-2016 | James Hall | Southern Kings | South Africa | 02-Jan-1996 | 20(98) |
| 26-Mar-2016 | Jason Jenkins | Bulls | South Africa | 02-Dec-1995 | 20(115) |
| 27-Feb-2016 | Sean Wainui | Crusaders | New Zealand | 23-Oct-1995 | 20(127) |
| 05-Mar-2016 | Leolin Zas | Western Stormers | South Africa | 20-Oct-1995 | 20(137) |
| 19-Mar-2016 | Sam McNicol | Chiefs | New Zealand | 06-Oct-1995 | 20(165) |
| 16-Apr-2016 | Andrew Kellaway | New South Wales | Australia | 12-Oct-1995 | 20(187) |
| 29-Apr-2016 | Hanro Liebenberg | Bulls | South Africa | 10-Oct-1995 | 20(202) |
| 27-Feb-2016 | Daniel du Preez | Sharks | South Africa | 05-Aug-1995 | 20(206) |
| 27-Feb-2016 | Jean Luc du Preez | Sharks | South Africa | 05-Aug-1995 | 20(206) |
| 14-May-2016 | Wes Goosen | Hurricanes | New Zealand | 20-Oct-1995 | 20(207) |
| 11-Mar-2016 | Jermaine Ainsley | Western Force | Australia | 08-Aug-1995 | 20(216) |
| 12-Mar-2016 | Ox Nche | Cheetahs | South Africa | 23-Jul-1995 | 20(233) |
| 27-Feb-2016 | Hyron Andrews | Sharks | South Africa | 06-Jul-1995 | 20(236) |
| 27-Feb-2016 | Malcolm Jaer | Southern Kings | South Africa | 29-Jun-1995 | 20(243) |
| 11-Mar-2016 | Akira Ioane | Blues | New Zealand | 16-Jun-1995 | 20(269) |
| 27-Feb-2016 | Warrick Gelant | Bulls | South Africa | 20-May-1995 | 20(283) |
| 05-Mar-2016 | Jonah Placid | Melbourne Rebels | Australia | 14-May-1995 | 20(296) |
| 08-Apr-2016 | Atunaisa Moli | Chiefs | New Zealand | 12-Jun-1995 | 20(301) |
| 27-Feb-2016 | Damian McKenzie | Chiefs | New Zealand | 20-Apr-1995 | 20(313) |
| 26-Feb-2016 | Blake Gibson | Blues | New Zealand | 19-Apr-1995 | 20(313) |
| 26-Mar-2016 | JD Schickerling | Western Stormers | South Africa | 09-May-1995 | 20(322) |
| 19-Mar-2016 | Anton Leinert-Brown | Chiefs | New Zealand | 15-Apr-1995 | 20(339) |
| 26-Feb-2016 | Tevita Li | Blues | New Zealand | 23-Mar-1995 | 20(340) |
| 26-Feb-2016 | Leni Apisai | Hurricanes | New Zealand | 08-Mar-1995 | 20(355) |
| 27-Feb-2016 | RG Snyman | Bulls | South Africa | 29-Jan-1995 | 21(29) |
| 25-Mar-2016 | CJ Velleman | Southern Kings | South Africa | 24-Feb-1995 | 21(30) |
| 26-Feb-2016 | Emiliano Boffelli | Jaguares | Argentina | 16-Jan-1995 | 21(41) |
| 26-Feb-2016 | Vince Aso | Hurricanes | New Zealand | 05-Jan-1995 | 21(52) |
| 16-Jul-2016 | Dewald Human | Southern Kings | South Africa | 19-May-1995 | 21(59) |
| 05-Mar-2016 | Latu Vaeno | Chiefs | Tonga | 05-Jan-1995 | 21(60) |
| 27-Feb-2016 | David Havili | Crusaders | New Zealand | 23-Dec-1994 | 21(66) |
| 15-Jul-2016 | Thomas du Toit | Sharks | South Africa | 05-May-1995 | 21(72) |
| 28-May-2016 | Daniel du Plessis | Western Stormers | South Africa | 17-Mar-1995 | 21(73) |
| 26-Feb-2016 | Guido Petti | Jaguares | Argentina | 17-Nov-1994 | 21(101) |
| 27-Feb-2016 | David Horwitz | New South Wales | Australia | 30-Sep-1994 | 21(150) |
| 01-Jul-2016 | Jack Tuttle | Queensland Reds | Australia | 02-Feb-1995 | 21(150) |
| 29-Apr-2016 | Harry Scobie | Western Force | Australia | 12-Nov-1994 | 21(169) |
| 08-Jul-2016 | Duncan Paia'aua | Queensland Reds | Samoa | 20-Jan-1995 | 21(170) |
| 30-Jul-2016 | Ricky Riccitelli | Hurricanes | New Zealand | 03-Feb-1995 | 21(178) |
| 12-Mar-2016 | Rohan Janse van Rensburg | Lions | South Africa | 11-Sep-1994 | 21(183) |
| 27-Mar-2016 | Junior Laloifi | Queensland Reds | Australia | 25-Sep-1994 | 21(184) |
| 27-Feb-2016 | Reece Hodge | Melbourne Rebels | Australia | 26-Aug-1994 | 21(185) |
| 26-Feb-2016 | Sergeal Petersen | Cheetahs | South Africa | 01-Aug-1994 | 21(209) |
| 16-Apr-2016 | Tom Robertson | New South Wales | Australia | 28-Aug-1994 | 21(232) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).