| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 16-May-2010 |  Ben Youngs | Leicester Tigers | England | 05-Sep-1989 | 20(253) |
| 16-May-2010 |  Andy Saull | Saracens | England | 27-Aug-1988 | 21(262) |
| 16-May-2010 | Alex Goode | Saracens | England | 07-May-1988 | 22(9) |
| 16-May-2010 |  Chris Ashton | Northampton Saints | England | 29-Mar-1987 | 23(48) |
| 16-May-2010 | Adam Powell | Saracens | England | 01-Jan-1987 | 23(135) |
| 16-May-2010 |  Matt Banahan | Bath | England | 30-Dec-1986 | 23(137) |
| 16-May-2010 | Anthony Allen | Leicester Tigers | England | 01-Sep-1986 | 23(257) |
| 16-May-2010 | Nick Abendanon | Bath | England | 27-Aug-1986 | 23(262) |
| 16-May-2010 | Bradley Barritt | Saracens | England | 07-Aug-1986 | 23(282) |
| 16-May-2010 | Jordan Crane | Leicester Tigers | England | 03-Jun-1986 | 23(347) |
| 16-May-2010 | Dylan Hartley | Northampton Saints | England | 24-Mar-1986 | 24(53) |
| 16-May-2010 | Matt Smith | Leicester Tigers | England | 15-Nov-1985 | 24(182) |
| 16-May-2010 | Julian Salvi | Bath | Australia | 09-Nov-1985 | 24(188) |
| 16-May-2010 | Tom Croft | Leicester Tigers | England | 07-Nov-1985 | 24(190) |
| 16-May-2010 | Petrus Johannes Juandre Kruger | Northampton Saints | South Africa | 06-Sep-1985 | 24(252) |
| 16-May-2010 | Toby Flood | Leicester Tigers | England | 08-Aug-1985 | 24(281) |
| 16-May-2010 | Ben Foden | Northampton Saints | England | 22-Jul-1985 | 24(298) |
| 16-May-2010 | Michael Tagicakibau | Saracens | Fiji | 09-May-1985 | 25(7) |
| 16-May-2010 | Dave Wilson | Bath | England | 09-Apr-1985 | 25(37) |
| 16-May-2010 | Lee Dickson | Northampton Saints | England | 29-Mar-1985 | 25(48) |
| 16-May-2010 | Brian Mujati | Northampton Saints | South Africa | 28-Sep-1984 | 25(230) |
| 16-May-2010 | Matt Carraro | Bath | Australia | 04-Aug-1984 | 25(285) |
| 16-May-2010 | Stephen Myler | Northampton Saints | England | 21-Jul-1984 | 25(299) |
| 16-May-2010 | Geoffrey Parling | Leicester Tigers | England | 28-Oct-1983 | 26(201) |
| 16-May-2010 | Luke Watson | Bath | South Africa | 26-Oct-1983 | 26(203) |
| 16-May-2010 | Jon Clarke | Northampton Saints | England | 22-Oct-1983 | 26(207) |
| 16-May-2010 | Chris Wyles | Saracens | United States | 13-Sep-1983 | 26(246) |
| 16-May-2010 | Jacques Burger | Saracens | Namibia | 29-Jul-1983 | 26(292) |
| 16-May-2010 | Marcos Ayerza | Leicester Tigers | Argentina | 12-Jan-1983 | 27(124) |
| 16-May-2010 | Michael Claassens | Bath | South Africa | 29-Jan-1982 | 28(107) |
| 16-May-2010 | Mouritz Botha | Saracens | England | 29-Jan-1982 | 28(107) |
| 16-May-2010 | Soane Tonga'uiha | Northampton Saints | Tonga | 21-Jan-1982 | 28(115) |
| 16-May-2010 | Olly Barkley | Bath | England | 28-Nov-1981 | 28(169) |
| 16-May-2010 | Stuart Hooper | Bath | England | 18-Nov-1981 | 28(179) |
| 16-May-2010 | Martin Castrogiovanni | Leicester Tigers | Italy | 21-Oct-1981 | 28(207) |
| 16-May-2010 | Phil Dowson | Northampton Saints | England | 01-Oct-1981 | 28(227) |
| 16-May-2010 | Roger Wilson | Northampton Saints | Ireland | 21-Sep-1981 | 28(237) |
| 16-May-2010 | Petrus Du Plessis | Saracens | England | 31-May-1981 | 28(350) |
| 16-May-2010 | Schalk Brits | Saracens | South Africa | 16-May-1981 | 29(0) |
| 16-May-2010 | James Downey | Northampton Saints | Ireland | 23-Mar-1981 | 29(54) |
| 16-May-2010 | Alesana Tuilagi | Leicester Tigers | Samoa | 24-Feb-1981 | 29(81) |
| 16-May-2010 | Matias Aguero | Saracens | Italy | 13-Feb-1981 | 29(92) |
| 16-May-2010 | Louis Deacon | Leicester Tigers | England | 07-Oct-1980 | 29(221) |
| 16-May-2010 | Ernst Joubert | Saracens | South Africa | 05-Aug-1980 | 29(284) |
| 16-May-2010 | Scott Hamilton | Leicester Tigers | New Zealand | 04-Mar-1980 | 30(73) |
| 16-May-2010 | David Flatman | Bath | England | 21-Jan-1980 | 30(116) |
| 29-May-2010 | Steve Borthwick | Saracens | England | 12-Oct-1979 | 30(230) |
| 16-May-2010 | Butch James | Bath | South Africa | 08-Jul-1979 | 30(313) |
| 16-May-2010 | Neil Best | Northampton Saints | Ireland | 03-Apr-1979 | 31(44) |
| 16-May-2010 | Lee Mears | Bath | England | 05-Mar-1979 | 31(73) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).