| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 20-Mar-2010 |  Tom Prydie | Wales | Wales | 23-Feb-1992 | 18(26) |
| 07-Feb-2010 |  Benjamin Fall | France | France | 29-Apr-1989 | 20(284) |
| 13-Feb-2010 | Leigh Halfpenny | Wales | Wales | 22-Dec-1988 | 21(53) |
| 07-Feb-2010 | Morgan Parra | France | France | 15-Nov-1988 | 21(84) |
| 07-Feb-2010 | Mathieu Bastareaud | France | France | 17-Sep-1988 | 21(143) |
| 20-Mar-2010 | Sam Warburton | Wales | Wales | 05-Oct-1988 | 21(166) |
| 06-Feb-2010 |  Cian Healy | Ireland | Ireland | 07-Oct-1987 | 22(123) |
| 13-Feb-2010 | Keith Earls | Ireland | Ireland | 02-Oct-1987 | 22(135) |
| 13-Feb-2010 | Alexis Palisson | France | France | 09-Sep-1987 | 22(158) |
| 06-Feb-2010 |  Luke McLean | Italy | Italy | 11-May-1987 | 22(272) |
| 14-Feb-2010 |  Dan Cole | England | England | 09-May-1987 | 22(282) |
| 06-Feb-2010 | Tom James | Wales | Wales | 17-Apr-1987 | 22(296) |
| 20-Mar-2010 | Chris Ashton | England | England | 29-Mar-1987 | 22(357) |
| 06-Feb-2010 | Danny Care | England | England | 02-Jan-1987 | 23(35) |
| 07-Feb-2010 | Francois Trinh Duc | France | France | 11-Nov-1986 | 23(88) |
| 06-Feb-2010 | Jamie Roberts | Wales | Wales | 08-Nov-1986 | 23(90) |
| 07-Feb-2010 |  John Barclay | Scotland | Scotland | 24-Sep-1986 | 23(136) |
| 07-Feb-2010 | Fulgence Ouedraogo | France | France | 21-Jul-1986 | 23(201) |
| 06-Feb-2010 | Dylan Hartley | England | England | 24-Mar-1986 | 23(319) |
| 06-Feb-2010 | Matthew Tait | England | England | 06-Feb-1986 | 24(0) |
| 14-Mar-2010 | Marc Andreu | France | France | 27-Dec-1985 | 24(77) |
| 07-Feb-2010 | John Beattie | Scotland | Scotland | 21-Nov-1985 | 24(78) |
| 06-Feb-2010 | Alan Wyn Jones | Wales | Wales | 19-Sep-1985 | 24(140) |
| 07-Feb-2010 | Thomas Domingo | France | France | 20-Aug-1985 | 24(171) |
| 06-Feb-2010 | Toby Flood | England | England | 08-Aug-1985 | 24(182) |
| 13-Feb-2010 | Stephen Ferris | Ireland | Ireland | 02-Aug-1985 | 24(195) |
| 06-Feb-2010 | James Hook | Wales | Wales | 27-Jun-1985 | 24(224) |
| 27-Feb-2010 | Jonathan Sexton | Ireland | Ireland | 11-Jul-1985 | 24(231) |
| 20-Mar-2010 | Ben Foden | England | England | 22-Jul-1985 | 24(241) |
| 06-Feb-2010 | Dave Wilson | England | England | 09-Apr-1985 | 24(303) |
| 06-Feb-2010 | James Haskell | England | England | 02-Apr-1985 | 24(310) |
| 07-Feb-2010 | Thom Evans | Scotland | Scotland | 02-Apr-1985 | 24(311) |
| 06-Feb-2010 | Leonardo Ghiraldini | Italy | Italy | 26-Dec-1984 | 25(42) |
| 26-Feb-2010 | Bradley Davies | Wales | Wales | 09-Jan-1985 | 25(48) |
| 07-Feb-2010 | Moray Low | Scotland | Scotland | 28-Nov-1984 | 25(71) |
| 06-Feb-2010 | Andrew Trimble | Ireland | Ireland | 20-Oct-1984 | 25(109) |
| 06-Feb-2010 | Kevin McLaughlin | Ireland | Ireland | 20-Sep-1984 | 25(139) |
| 07-Feb-2010 | Ross Ford | Scotland | Scotland | 23-Apr-1984 | 25(290) |
| 06-Feb-2010 | Tommy Bowe | Ireland | Ireland | 22-Feb-1984 | 25(350) |
| 06-Feb-2010 | Gonzalo Garcia | Italy | Italy | 18-Feb-1984 | 25(354) |
| 06-Feb-2010 | Alessandro Zanni | Italy | Italy | 31-Jan-1984 | 26(7) |
| 13-Mar-2010 | Nick De Luca | Scotland | Scotland | 01-Feb-1984 | 26(41) |
| 06-Feb-2010 | Delon Armitage | England | England | 15-Dec-1983 | 26(54) |
| 06-Feb-2010 | James Heaslip | Ireland | Ireland | 15-Dec-1983 | 26(54) |
| 06-Feb-2010 | Tomas O'Leary | Ireland | Ireland | 22-Oct-1983 | 26(108) |
| 07-Feb-2010 | Max Evans | Scotland | Scotland | 28-Sep-1983 | 26(133) |
| 07-Feb-2010 | Alasdair Dickinson | Scotland | Scotland | 11-Sep-1983 | 26(150) |
| 26-Feb-2010 | Huw Bennett | Wales | Wales | 11-Jun-1983 | 26(261) |
| 26-Feb-2010 | Richie Rees | Wales | Wales | 21-May-1983 | 26(282) |
| 26-Feb-2010 | Julien Malzieu | France | France | 04-May-1983 | 26(299) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).