| MD | Players | Nationality | DOB | A(D) |
|---|---|---|---|---|
| 14-Nov-2009 | Guy Mercer | England | 12-Dec-1989 | 19(337) |
| 08-Nov-2009 | Josh Ovens | England | 11-Jul-1989 | 20(120) |
| 08-Nov-2009 | Ben Williams | England | 28-May-1989 | 20(164) |
| 14-Nov-2009 | Nathan Catt | England | 06-Jan-1988 | 21(313) |
| 08-Nov-2009 | Jack Cuthbert | Scotland | 03-Sep-1987 | 22(67) |
| 06-Feb-2010 | Nick Abendanon | England | 27-Aug-1986 | 23(163) |
| 08-Nov-2009 | Aaron Jarvis | Wales | 20-May-1986 | 23(172) |
| 08-Nov-2009 | Tom Cheeseman | Wales | 22-Mar-1986 | 23(231) |
| 14-Nov-2009 | Ryan Davis | England | 11-Nov-1985 | 24(3) |
| 08-Nov-2009 | Rhys Crane | England | 14-Oct-1985 | 24(25) |
| 14-Nov-2009 | Mike Baxter | England | 16-Dec-1984 | 24(333) |
| 06-Feb-2010 | Matt Carraro | Australia | 04-Aug-1984 | 25(186) |
| 08-Nov-2009 | Jonathan Faamatuainu | Samoa | 29-Dec-1983 | 25(315) |
| 14-Nov-2009 | Ben Skirving | England | 09-Oct-1983 | 26(37) |
| 08-Nov-2009 | Rob Hawkins | England | 14-Apr-1983 | 26(209) |
| 06-Feb-2010 | Stuart Hooper | England | 18-Nov-1981 | 28(80) |
| 08-Nov-2009 | Michael Stephenson | England | 20-Sep-1980 | 29(49) |
| 08-Nov-2009 | Scott Hobson | England | 25-Jan-1980 | 29(288) |
| 08-Nov-2009 | Peter Short | England | 20-Jun-1979 | 30(142) |
| 08-Nov-2009 | Daniel Browne | Ireland | 13-Apr-1979 | 30(210) |
| 06-Feb-2010 | Butch James | South Africa | 08-Jul-1979 | 30(214) |
| 14-Nov-2009 | Joe Maddock | New Zealand | 20-Dec-1978 | 30(330) |
| 08-Nov-2009 | Scott Bemand | England | 21-Sep-1978 | 31(49) |
| 14-Nov-2009 | Andy Beattie | England | 06-Sep-1978 | 31(70) |
| 29-Jan-2010 | Pieter Dixon | Zimbabwe | 17-Oct-1977 | 32(104) |
| 08-Nov-2009 | Nicky Little | Fiji | 13-Sep-1976 | 33(56) |
| 08-Nov-2009 | David Barnes | England | 12-Jul-1976 | 33(119) |
| 29-Jan-2010 | Duncan Bell | England | 01-Oct-1974 | 35(121) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).