| MD | Players | Nationality | DOB | A(D) |
|---|---|---|---|---|
| 15-Nov-2009 | Jack Wallace | England | 22-Jan-1991 | 18(298) |
| 08-Nov-2009 | Bob Baker | England | 12-Sep-1989 | 20(57) |
| 08-Nov-2009 | Hugo Ellis | England | 04-Feb-1988 | 21(278) |
| 07-Feb-2010 | Mark Odejobi | England | 09-Mar-1988 | 21(335) |
| 08-Nov-2009 | Lachlan Mitchell | Australia | 30-Sep-1987 | 22(40) |
| 08-Nov-2009 | Charlie Beech | England | 21-Jul-1987 | 22(111) |
| 07-Feb-2010 | Lee Smith | England | 08-Aug-1986 | 23(183) |
| 08-Nov-2009 | Warren Fury | Wales | 10-Dec-1985 | 23(333) |
| 08-Nov-2009 | Tom Varndell | England | 16-Aug-1985 | 24(84) |
| 15-Nov-2009 | Will Matthews | England | 14-Jan-1985 | 24(305) |
| 08-Nov-2009 | George Skivington | England | 03-Dec-1982 | 26(341) |
| 30-Jan-2010 | Sakaria Taulafo | Samoa | 29-Jan-1983 | 27(1) |
| 15-Nov-2009 | Daniel Leo | Samoa | 02-Oct-1982 | 27(44) |
| 08-Nov-2009 | Ben Jacobs | Australia | 17-May-1982 | 27(175) |
| 15-Nov-2009 | Joe Horn-Smith | England | 30-Apr-1982 | 27(199) |
| 08-Nov-2009 | John Hart | England | 20-Mar-1982 | 27(233) |
| 30-Jan-2010 | Ben Broster | Wales | 07-May-1982 | 27(268) |
| 08-Nov-2009 | David Lemi | Samoa | 10-Feb-1982 | 27(271) |
| 15-Nov-2009 | Eoghan Hickey | Ireland | 29-Oct-1981 | 28(17) |
| 08-Nov-2009 | Joe Ward | England | 03-Jun-1980 | 29(158) |
| 15-Nov-2009 | Paul Sackey | England | 08-Nov-1979 | 30(8) |
| 15-Nov-2009 | Richard Birkett | England | 01-Oct-1979 | 30(46) |
| 30-Jan-2010 | Steve Kefu | Australia | 06-Dec-1979 | 30(56) |
| 08-Nov-2009 | David Walder | England | 07-May-1978 | 31(186) |
| 07-Feb-2010 | Dan Ward-Smith | England | 02-Jan-1978 | 32(36) |
| 08-Nov-2009 | Mark van Gisbergen | England | 30-Jun-1977 | 32(131) |
| 08-Nov-2009 | Joe Worsley | England | 14-Jun-1977 | 32(147) |
| 30-Jan-2010 | Marty Veale | England | 09-Aug-1977 | 32(174) |
| 07-Feb-2010 | Mark Robinson | New Zealand | 21-Aug-1975 | 34(171) |
| 08-Nov-2009 | Simon Shaw | England | 01-Sep-1973 | 36(68) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).