| MD | Players | Nationality | DOB | A(D) |
|---|---|---|---|---|
| 15-Nov-2009 | Jonathan Joseph | England | 21-May-1991 | 18(179) |
| 08-Nov-2009 | Tom Homer | England | 01-Apr-1990 | 19(221) |
| 08-Nov-2009 | Jon Fisher | England | 15-Sep-1988 | 21(54) |
| 08-Nov-2009 | Alex Corbisiero | England | 30-Aug-1988 | 21(70) |
| 08-Nov-2009 | Charlie Gower | England | 28-Sep-1987 | 22(42) |
| 06-Feb-2010 | Matthew Garvey | England | 23-Oct-1987 | 22(107) |
| 08-Nov-2009 | Jamie Lennard | England | 23-Feb-1987 | 22(259) |
| 31-Jan-2010 | Ryan Lamb | England | 18-May-1986 | 23(258) |
| 08-Nov-2009 | Dan Murphy | England | 27-Oct-1985 | 24(12) |
| 15-Nov-2009 | Steffon Armitage | England | 20-Sep-1985 | 24(56) |
| 31-Jan-2010 | Topsy Ojo | England | 28-Jul-1985 | 24(187) |
| 08-Nov-2009 | Richard Thorpe | Canada | 01-Nov-1984 | 25(7) |
| 08-Nov-2009 | Gary Johnson | England | 24-Apr-1984 | 25(198) |
| 08-Nov-2009 | David Paice | England | 24-Nov-1983 | 25(350) |
| 06-Feb-2010 | Alfredo Lalanne | Argentina | 03-Mar-1983 | 26(341) |
| 31-Jan-2010 | Paulica Ion | Romania | 10-Jan-1983 | 27(21) |
| 31-Jan-2010 | Sailosi Tagicakibau | Samoa | 14-Nov-1982 | 27(78) |
| 31-Jan-2010 | James Buckland | England | 21-Sep-1981 | 28(132) |
| 08-Nov-2009 | John Rudd | England | 26-May-1981 | 28(166) |
| 15-Nov-2009 | Clarke Dermody | New Zealand | 22-Apr-1980 | 29(207) |
| 06-Feb-2010 | Seilala Mapusua | Samoa | 27-Feb-1980 | 29(345) |
| 08-Nov-2009 | Kieran Roche | England | 03-May-1979 | 30(190) |
| 31-Jan-2010 | George Stowers | Fiji | 14-Feb-1979 | 30(352) |
| 06-Feb-2010 | Elvis Seveali'i | Samoa | 20-Jun-1978 | 31(231) |
| 08-Nov-2009 | Peter Hewat | Australia | 17-Mar-1978 | 31(236) |
| 08-Nov-2009 | Peter Richards | England | 10-Mar-1978 | 31(244) |
| 08-Nov-2009 | Chris Malone | Australia | 08-Jan-1978 | 31(305) |
| 06-Feb-2010 | Faan Rautenbach | South Africa | 22-Feb-1976 | 33(350) |
| 08-Nov-2009 | Andy Perry | England | 28-Dec-1974 | 34(316) |
| 08-Nov-2009 | Mike Catt | England | 17-Sep-1971 | 38(53) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).