| MD | Players | Nationality | DOB | A(D) |
|---|---|---|---|---|
| 08-Nov-2009 | George Ford | England | 16-Mar-1993 | 16(237) |
| 08-Nov-2009 | Alex Lewington | England | 20-Sep-1991 | 18(50) |
| 08-Nov-2009 | Joe Cobden | England | 01-Sep-1991 | 18(69) |
| 08-Nov-2009 | Matt Everard | England | 09-Jan-1991 | 18(304) |
| 08-Nov-2009 | Tom Armes | England | 19-Sep-1990 | 19(50) |
| 14-Nov-2009 | Andrew Forsyth | England | 09-Sep-1990 | 19(66) |
| 29-Jan-2010 | Callum Green | England | 15-Jul-1990 | 19(198) |
| 08-Nov-2009 | Will Hurrell | England | 15-Jan-1990 | 19(297) |
| 14-Nov-2009 | Ben Youngs | England | 05-Sep-1989 | 20(70) |
| 08-Nov-2009 | Greig Tonks | Scotland | 20-May-1989 | 20(172) |
| 06-Feb-2010 | Dan Hemingway | England | 03-Jun-1989 | 20(248) |
| 29-Jan-2010 | Billy Twelvetrees | England | 15-Nov-1988 | 21(75) |
| 08-Nov-2009 | Dan Cole | England | 09-May-1987 | 22(184) |
| 08-Nov-2009 | Tom Youngs | England | 28-Jan-1987 | 22(285) |
| 14-Nov-2009 | Ben Pienaar | England | 10-Sep-1986 | 23(65) |
| 14-Nov-2009 | Anthony Allen | England | 01-Sep-1986 | 23(74) |
| 14-Nov-2009 | Jordan Crane | England | 03-Jun-1986 | 23(164) |
| 08-Nov-2009 | Lucas Gonzalez Amorosino | Argentina | 02-Nov-1985 | 24(6) |
| 29-Jan-2010 | Matt Smith | England | 15-Nov-1985 | 24(75) |
| 14-Nov-2009 | Geoffrey Parling | England | 28-Oct-1983 | 26(18) |
| 08-Nov-2009 | Sam Raven | England | 21-Dec-1982 | 26(323) |
| 29-Jan-2010 | Joe Duffey | New Zealand | 22-Jun-1982 | 27(221) |
| 08-Nov-2009 | Robert Lee Harris | South Africa | 30-Mar-1982 | 27(223) |
| 29-Jan-2010 | Ben Woods | England | 09-Jun-1982 | 27(234) |
| 29-Jan-2010 | Harry Ellis | England | 17-May-1982 | 27(257) |
| 29-Jan-2010 | Brett Deacon | England | 07-Mar-1982 | 27(328) |
| 29-Jan-2010 | Sam Vesty | England | 26-Nov-1981 | 28(64) |
| 29-Jan-2010 | Alesana Tuilagi | Samoa | 24-Feb-1981 | 28(339) |
| 29-Jan-2010 | Aaron Mauger | New Zealand | 29-Nov-1980 | 29(61) |
| 14-Nov-2009 | Boris Stankovich | England | 01-Sep-1980 | 29(74) |
| 08-Nov-2009 | James Grindal | England | 18-Aug-1980 | 29(82) |
| 14-Nov-2009 | Jeremy Staunton | Ireland | 07-May-1980 | 29(191) |
| 14-Nov-2009 | Scott Hamilton | New Zealand | 04-Mar-1980 | 29(255) |
| 14-Nov-2009 | Lote Tuqiri | Australia | 23-Sep-1979 | 30(53) |
| 14-Nov-2009 | Craig Newby | New Zealand | 27-Jul-1979 | 30(111) |
| 08-Nov-2009 | Craig Hammond | Other | 11-Feb-1979 | 30(271) |
| 14-Nov-2009 | George Chuter | England | 09-Jul-1976 | 33(128) |
| 08-Nov-2009 | Ben Kay | England | 14-Dec-1975 | 33(330) |
| 29-Jan-2010 | Julian White | England | 14-May-1973 | 36(260) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).