| MD | Players | Nationality | DOB | A(D) |
|---|---|---|---|---|
| 08-Nov-2009 | Freddie Burns | England | 13-May-1990 | 19(179) |
| 15-Nov-2009 | Jonny May | England | 01-Apr-1990 | 19(228) |
| 30-Jan-2010 | Yann Thomas | England | 16-Apr-1990 | 19(289) |
| 30-Jan-2010 | Charles Sharples | England | 17-Aug-1989 | 20(166) |
| 08-Nov-2009 | Henry Trinder | England | 14-Apr-1989 | 20(208) |
| 30-Jan-2010 | David Lewis | England | 29-Apr-1989 | 20(276) |
| 07-Feb-2010 | Darren Dawiduik | England | 21-Sep-1987 | 22(140) |
| 08-Nov-2009 | David Attwood | England | 05-Apr-1987 | 22(218) |
| 15-Nov-2009 | Olly Morgan | England | 03-Nov-1985 | 24(12) |
| 08-Nov-2009 | Paul Doran-Jones | England | 02-May-1985 | 24(190) |
| 07-Feb-2010 | Rupert Harden | England | 09-May-1985 | 24(274) |
| 30-Jan-2010 | Akapusi Qera | Fiji | 24-Apr-1984 | 25(281) |
| 07-Feb-2010 | Luke Narraway | England | 07-Sep-1983 | 26(154) |
| 08-Nov-2009 | Alasdair Strokosch | Scotland | 21-Feb-1983 | 26(261) |
| 15-Nov-2009 | Nick Wood | England | 09-Jan-1983 | 26(311) |
| 08-Nov-2009 | Gareth Delve | Wales | 30-Dec-1982 | 26(314) |
| 07-Feb-2010 | Tim Taylor | England | 04-Nov-1982 | 27(95) |
| 08-Nov-2009 | James Simpson-Daniel | England | 30-May-1982 | 27(162) |
| 08-Nov-2009 | Nicky Robinson | Wales | 03-Jan-1982 | 27(309) |
| 08-Nov-2009 | Scott Lawson | Scotland | 28-Sep-1981 | 28(41) |
| 15-Nov-2009 | Ben Phillips | Wales | 05-Sep-1981 | 28(71) |
| 08-Nov-2009 | Rory Lawson | Scotland | 12-Mar-1981 | 28(241) |
| 08-Nov-2009 | Tim Molenaar | New Zealand | 19-Feb-1981 | 28(262) |
| 15-Nov-2009 | Tom Voyce | England | 05-Jan-1981 | 28(314) |
| 15-Nov-2009 | Eliota Fuimaono Sapolu | Samoa | 31-Oct-1980 | 29(15) |
| 30-Jan-2010 | Marco Bortolami | Italy | 12-Jun-1980 | 29(232) |
| 08-Nov-2009 | Lesley Vainikolo | England | 04-May-1979 | 30(189) |
| 30-Jan-2010 | Alex Brown | England | 17-May-1979 | 30(259) |
| 08-Nov-2009 | Adam Eustace | England | 09-Jan-1979 | 30(304) |
| 15-Nov-2009 | Peter Buxton | England | 21-Aug-1978 | 31(86) |
| 15-Nov-2009 | Apolosi Satala | Fiji | 14-Aug-1978 | 31(94) |
| 14-Mar-2010 | Mike Tindall | England | 18-Oct-1978 | 31(147) |
| 07-Feb-2010 | Andrew Hazell | England | 25-Apr-1978 | 31(289) |
| 07-Feb-2010 | Semi Tadulala | Fiji | 03-Mar-1978 | 31(342) |
| 08-Nov-2009 | Greg Somerville | New Zealand | 28-Nov-1977 | 31(345) |
| 08-Nov-2009 | William James | Wales | 22-Dec-1976 | 32(321) |
| 14-Mar-2010 | Jake Boer | South Africa | 01-Nov-1975 | 34(134) |
| 30-Jan-2010 | Olivier Azam | France | 21-Oct-1974 | 35(102) |
| 15-Nov-2009 | Pierre Capdevielle | France | 30-Mar-1974 | 35(231) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).