| MD | Players | Nationality | DOB | A(D) |
|---|---|---|---|---|
| 11-Oct-2009 | Jake Sharp | England | 07-May-1991 | 18(158) |
| 15-Oct-2009 | Andy Saull | England | 27-Aug-1988 | 21(49) |
| 19-Dec-2009 | Alex Goode | England | 07-May-1988 | 21(226) |
| 11-Dec-2009 | Noah Cato | England | 31-Mar-1988 | 21(255) |
| 19-Dec-2009 | Tom Mercey | England | 15-Jun-1987 | 22(188) |
| 11-Dec-2009 | Adam Powell | England | 01-Jan-1987 | 22(345) |
| 11-Dec-2009 | Rhys Gill | Wales | 30-Oct-1986 | 23(42) |
| 15-Oct-2009 | Bradley Barritt | England | 07-Aug-1986 | 23(69) |
| 11-Oct-2009 | Don Barrell | England | 03-Feb-1986 | 23(250) |
| 11-Oct-2009 | Michael Tagicakibau | Fiji | 09-May-1985 | 24(155) |
| 11-Oct-2009 | Hayden Smith | United States | 10-Apr-1985 | 24(184) |
| 11-Oct-2009 | Tom Ryder | Scotland | 21-Feb-1985 | 24(232) |
| 15-Oct-2009 | Chris Wyles | United States | 13-Sep-1983 | 26(33) |
| 11-Oct-2009 | Justin Melck | South Africa | 04-Jan-1983 | 26(281) |
| 15-Oct-2009 | Derick Hougaard | South Africa | 04-Jan-1983 | 26(285) |
| 11-Dec-2009 | Kameli Ratuvou | Fiji | 06-Nov-1982 | 27(35) |
| 15-Oct-2009 | Ethienne Reynecke | South Africa | 20-Mar-1982 | 27(209) |
| 19-Dec-2009 | Mouritz Botha | England | 29-Jan-1982 | 27(324) |
| 11-Oct-2009 | Matias Aguero | Italy | 13-Feb-1981 | 28(240) |
| 14-Jan-2010 | Schalk Brits | South Africa | 16-May-1981 | 28(243) |
| 11-Oct-2009 | Richard Haughton | England | 08-Nov-1980 | 28(337) |
| 11-Oct-2009 | Michael Owen | Wales | 07-Nov-1980 | 28(338) |
| 11-Oct-2009 | Richard Skuse | England | 11-Sep-1980 | 29(30) |
| 11-Oct-2009 | Kevin Barrett | Ireland | 06-Jul-1980 | 29(97) |
| 11-Dec-2009 | Ernst Joubert | South Africa | 05-Aug-1980 | 29(128) |
| 15-Oct-2009 | Steve Borthwick | England | 12-Oct-1979 | 30(4) |
| 15-Oct-2009 | Wikus van Heerden | South Africa | 25-Feb-1979 | 30(233) |
| 11-Oct-2009 | Rod Penney | England | 22-Jul-1978 | 31(81) |
| 11-Oct-2009 | Fabio Ongaro | Italy | 23-Sep-1977 | 32(18) |
| 11-Oct-2009 | Michael Horak | England | 03-Jun-1977 | 32(130) |
| 11-Oct-2009 | Kevin Sorrell | England | 06-Mar-1977 | 32(219) |
| 19-Dec-2009 | Hugh Vyvyan | England | 08-Sep-1976 | 33(102) |
| 11-Dec-2009 | Carlos Nieto | Italy | 28-Apr-1976 | 33(227) |
| 11-Dec-2009 | Glen Jackson | New Zealand | 23-Oct-1975 | 34(50) |
| 11-Dec-2009 | Mosese Raulini | Fiji | 27-Jun-1975 | 34(168) |
| 15-Oct-2009 | Justin Marshall | New Zealand | 05-Aug-1973 | 36(71) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).