| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 09-Oct-2009 |  Geoffrey Palis | Albi | France | 08-Jul-1991 | 18(94) |
| 11-Oct-2009 |  Jake Sharp | Saracens | England | 07-May-1991 | 18(158) |
| 09-Oct-2009 | Tiernan O'Halloran | Connacht | Ireland | 26-Feb-1991 | 18(226) |
| 11-Oct-2009 | Edoardo Lubian | Rovigo | Italy | 11-Oct-1990 | 19(0) |
| 18-Oct-2009 | Tommaso Iannone | Parma | Italy | 16-Sep-1990 | 19(32) |
| 12-Dec-2009 | Constantino Ricciardi | Padova | Italy | 25-Aug-1990 | 19(109) |
| 09-Oct-2009 | Clement Lagain | Bayonne | France | 02-Jun-1990 | 19(129) |
| 09-Oct-2009 | Julien Larroque | Montauban | France | 28-Apr-1990 | 19(164) |
| 19-Dec-2009 | Jeremy Sinzelle | Toulon | France | 02-Jul-1990 | 19(170) |
| 18-Oct-2009 |  Will Welch | Newcastle Falcons | England | 03-Apr-1990 | 19(198) |
| 12-Dec-2009 | Enrico Targa | Padova | Italy | 24-May-1990 | 19(202) |
| 18-Dec-2009 | Michael Tait | Newcastle Falcons | Scotland | 07-May-1990 | 19(225) |
| 19-Dec-2009 | Gabriele Cicchinelli | Roma | Italy | 28-Feb-1990 | 19(294) |
| 09-Oct-2009 | Philip Swainston | Leeds Tykes | England | 12-Dec-1989 | 19(301) |
| 10-Apr-2010 |  Tavis Knoyle | Scarlets | Wales | 02-Jun-1990 | 19(312) |
| 12-Dec-2009 | Jacob Rowan | Leeds Tykes | England | 14-Jan-1990 | 19(332) |
| 11-Apr-2010 |  Freddie Burns | Gloucester | England | 13-May-1990 | 19(333) |
| 08-Oct-2009 |  Graham Kitchener | Worcester Warriors | England | 29-Sep-1989 | 20(9) |
| 11-Apr-2010 | Jonny May | Gloucester | England | 01-Apr-1990 | 20(10) |
| 10-Oct-2009 | Robert Miller | Newcastle Falcons | England | 05-Aug-1989 | 20(66) |
| 19-Dec-2009 | Geoffrery Doumayrou | Montpellier | France | 16-Sep-1989 | 20(94) |
| 09-Oct-2009 | Calum Clark | Leeds Tykes | England | 10-Jun-1989 | 20(121) |
| 14-Jan-2010 |  Bob Baker | London Wasps | England | 12-Sep-1989 | 20(124) |
| 09-Oct-2009 | Benjamin Fall | Bayonne | France | 29-Apr-1989 | 20(163) |
| 08-Oct-2009 | Mickael De Marco | Montpellier | Spain | 22-Apr-1989 | 20(169) |
| 19-Dec-2009 | Carlo Vannini | Roma | Italy | 22-Jun-1989 | 20(180) |
| 10-Apr-2010 | Alexandre Dumoulin | Bourgoin | France | 24-Aug-1989 | 20(229) |
| 09-Oct-2009 | Cyril Montet | Montauban | France | 06-Feb-1989 | 20(245) |
| 17-Dec-2009 | Lorenzo Romano | Parma | Italy | 31-Mar-1989 | 20(261) |
| 10-Oct-2009 | Michael Young | Newcastle Falcons | England | 31-Dec-1988 | 20(283) |
| 14-Jan-2010 | Madalin Lemnaru | Bucuresti | Romania | 26-Mar-1989 | 20(294) |
| 08-Oct-2009 | John Arr | Worcester Warriors | England | 29-Nov-1988 | 20(313) |
| 12-Dec-2009 | Pierre Bernard | Bayonne | France | 31-Jan-1989 | 20(315) |
| 11-Oct-2009 | Andrea Pratichetti | Rovigo | Italy | 26-Nov-1988 | 20(319) |
| 11-Oct-2009 | Andrea De Marchi | Rovigo | Italy | 19-Nov-1988 | 20(326) |
| 09-Oct-2009 | Clement Maynadier | Albi | France | 11-Oct-1988 | 20(363) |
| 17-Oct-2009 | George Crook | Worcester Warriors | England | 13-Oct-1988 | 21(4) |
| 09-Oct-2009 | Paul Bonnefond | Castres | France | 13-Sep-1988 | 21(26) |
| 10-Oct-2009 | Dario Chistolini | Padova | Italy | 14-Sep-1988 | 21(26) |
| 12-Dec-2009 | Simone Favaro | Parma | Italy | 07-Nov-1988 | 21(35) |
| 15-Oct-2009 | Andy Saull | Saracens | England | 27-Aug-1988 | 21(49) |
| 23-Jan-2010 | Marc-Antoine Rallier | Castres | France | 02-Dec-1988 | 21(52) |
| 09-Oct-2009 | Cedric Coll | Montauban | France | 08-Aug-1988 | 21(62) |
| 11-Oct-2009 | Marcello De Gasperi | Rovigo | Italy | 12-Jul-1988 | 21(91) |
| 11-Oct-2009 | Joe Simpson | London Wasps | England | 05-Jul-1988 | 21(98) |
| 11-Oct-2009 | Andrea Bacchetti | Rovigo | Italy | 04-Jul-1988 | 21(99) |
| 17-Oct-2009 | Tommaso D'Apice | Roma | Italy | 30-Jun-1988 | 21(109) |
| 11-Apr-2010 |  Leigh Halfpenny | Cardiff Blues | Wales | 22-Dec-1988 | 21(110) |
| 14-Jan-2010 | Valentin Ivan | Bucuresti | Romania | 16-Sep-1988 | 21(120) |
| 08-Oct-2009 | Miles Benjamin | Worcester Warriors | England | 11-May-1988 | 21(150) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).