| MD | Players | Nationality | DOB | A(D) |
|---|---|---|---|---|
| 11-Oct-2009 | James Gaskell | England | 20-May-1990 | 19(144) |
| 11-Oct-2009 | Jonny Kennedy | Scotland | 16-Feb-1990 | 19(237) |
| 11-Oct-2009 | Carl Fearns | England | 25-May-1989 | 20(139) |
| 16-Oct-2009 | Marc Jones | Wales | 03-Apr-1987 | 22(197) |
| 11-Oct-2009 | Jack Forster | England | 19-Mar-1987 | 22(207) |
| 24-Jan-2010 | David Doherty | England | 28-Jan-1987 | 22(362) |
| 11-Oct-2009 | Anitele'a Tuilagi | Samoa | 06-May-1986 | 23(158) |
| 11-Oct-2009 | Robert O'Donnell | England | 15-Dec-1985 | 23(300) |
| 13-Dec-2009 | Matthew Tait | England | 06-Feb-1986 | 23(310) |
| 13-Dec-2009 | Neil Briggs | England | 01-Jun-1985 | 24(195) |
| 11-Oct-2009 | Sean Cox | England | 16-Jan-1985 | 24(268) |
| 11-Oct-2009 | David Seymour | England | 27-Sep-1984 | 25(14) |
| 16-Jan-2010 | Martin Halsall | England | 25-Dec-1984 | 25(22) |
| 16-Oct-2009 | Lee Thomas | Wales | 02-Jun-1984 | 25(136) |
| 20-Dec-2009 | Luke Abraham | England | 26-Sep-1983 | 26(86) |
| 11-Oct-2009 | Nick MacLeod | Wales | 22-Mar-1983 | 26(204) |
| 24-Jan-2010 | Richard Wigglesworth | England | 09-Jun-1983 | 26(230) |
| 11-Oct-2009 | Dwayne Peel | Wales | 31-Aug-1981 | 28(41) |
| 11-Oct-2009 | Marika Vakacegu | Fiji | 23-Jun-1981 | 28(110) |
| 16-Oct-2009 | Eifion Roberts | Wales | 13-Feb-1981 | 28(245) |
| 16-Oct-2009 | Charlie Hodgson | England | 12-Nov-1980 | 28(338) |
| 20-Dec-2009 | Chris Jones | England | 24-Aug-1980 | 29(118) |
| 16-Oct-2009 | Sisa Koyamaibole | Fiji | 06-Mar-1980 | 29(224) |
| 16-Oct-2009 | Mark Cueto | England | 26-Dec-1979 | 29(295) |
| 16-Oct-2009 | Andrew Sheridan | England | 01-Nov-1979 | 29(350) |
| 16-Oct-2009 | Dean Schofield | England | 19-Jan-1979 | 30(271) |
| 11-Oct-2009 | Mahonri Schwalger | Samoa | 15-Sep-1978 | 31(26) |
| 11-Oct-2009 | Ben Cohen | England | 14-Sep-1978 | 31(28) |
| 11-Oct-2009 | Gavin Kerr | Scotland | 03-Apr-1977 | 32(191) |
| 11-Oct-2009 | Oriol Ripol | Spain | 06-Sep-1975 | 34(36) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).