| MD | Players | Nationality | DOB | A(D) |
|---|---|---|---|---|
| 09-Oct-2009 | Matt Banahan | England | 30-Dec-1986 | 22(284) |
| 09-Oct-2009 | Nick Abendanon | England | 27-Aug-1986 | 23(43) |
| 13-Dec-2009 | Tom Cheeseman | Wales | 22-Mar-1986 | 23(266) |
| 09-Oct-2009 | Ryan Davis | England | 11-Nov-1985 | 23(332) |
| 09-Oct-2009 | Julian Salvi | Australia | 09-Nov-1985 | 23(334) |
| 13-Dec-2009 | Dave Wilson | England | 09-Apr-1985 | 24(248) |
| 09-Oct-2009 | Matt Carraro | Australia | 04-Aug-1984 | 25(66) |
| 09-Oct-2009 | Jonathan Faamatuainu | Samoa | 29-Dec-1983 | 25(285) |
| 18-Oct-2009 | Ben Skirving | England | 09-Oct-1983 | 26(10) |
| 13-Dec-2009 | Luke Watson | South Africa | 26-Oct-1983 | 26(49) |
| 09-Oct-2009 | Shontayne Hape | England | 30-Jan-1982 | 27(252) |
| 09-Oct-2009 | Michael Claassens | South Africa | 29-Jan-1982 | 27(253) |
| 09-Oct-2009 | Stuart Hooper | England | 18-Nov-1981 | 27(325) |
| 13-Dec-2009 | Michael Stephenson | England | 20-Sep-1980 | 29(84) |
| 09-Oct-2009 | David Flatman | England | 21-Jan-1980 | 29(262) |
| 13-Dec-2009 | Peter Short | England | 20-Jun-1979 | 30(177) |
| 09-Oct-2009 | Lee Mears | England | 05-Mar-1979 | 30(219) |
| 09-Oct-2009 | Joe Maddock | New Zealand | 20-Dec-1978 | 30(294) |
| 09-Oct-2009 | Andy Beattie | England | 06-Sep-1978 | 31(33) |
| 18-Oct-2009 | Pieter Dixon | Zimbabwe | 17-Oct-1977 | 32(1) |
| 18-Oct-2009 | Nicky Little | Fiji | 13-Sep-1976 | 33(35) |
| 09-Oct-2009 | David Barnes | England | 12-Jul-1976 | 33(89) |
| 09-Oct-2009 | Duncan Bell | England | 01-Oct-1974 | 35(9) |
| 09-Oct-2009 | Danny Grewcock | England | 07-Nov-1972 | 36(336) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).