| MD | Players | Nationality | DOB | A(D) |
|---|---|---|---|---|
| 10-Oct-2009 | Courtney Lawes | England | 23-Feb-1989 | 20(229) |
| 10-Oct-2009 | Chris Ashton | England | 29-Mar-1987 | 22(196) |
| 10-Oct-2009 | Shane Geraghty | England | 12-Aug-1986 | 23(59) |
| 10-Oct-2009 | Dylan Hartley | England | 24-Mar-1986 | 23(200) |
| 10-Oct-2009 | Petrus Johannes Juandre Kruger | South Africa | 06-Sep-1985 | 24(34) |
| 12-Dec-2009 | Joe Ansbro | Scotland | 29-Oct-1985 | 24(44) |
| 10-Oct-2009 | Ben Foden | England | 22-Jul-1985 | 24(80) |
| 10-Oct-2009 | Lee Dickson | England | 29-Mar-1985 | 24(195) |
| 17-Jan-2010 | Brian Mujati | South Africa | 28-Sep-1984 | 25(111) |
| 19-Dec-2009 | Stephen Myler | England | 21-Jul-1984 | 25(151) |
| 10-Oct-2009 | Jon Clarke | England | 22-Oct-1983 | 25(354) |
| 12-Dec-2009 | Christian Day | England | 24-Jun-1983 | 26(172) |
| 10-Oct-2009 | Soane Tonga'uiha | Tonga | 21-Jan-1982 | 27(262) |
| 10-Oct-2009 | Phil Dowson | England | 01-Oct-1981 | 28(9) |
| 10-Oct-2009 | Roger Wilson | Ireland | 21-Sep-1981 | 28(19) |
| 10-Oct-2009 | James Downey | Ireland | 23-Mar-1981 | 28(201) |
| 12-Dec-2009 | Euan Murray | Scotland | 07-Aug-1980 | 29(127) |
| 10-Oct-2009 | Neil Best | Ireland | 03-Apr-1979 | 30(191) |
| 10-Oct-2009 | Bruce Reihana | New Zealand | 06-Apr-1976 | 33(187) |
| 10-Oct-2009 | Santigo Bonorino | Argentina | 05-May-1975 | 34(159) |
| 19-Dec-2009 | Ignacio Fernandez Lobbe | Argentina | 20-Nov-1974 | 35(30) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).