| MD | Players | Nationality | DOB | A(D) |
|---|---|---|---|---|
| 19-Dec-2009 | Ben Youngs | England | 05-Sep-1989 | 20(105) |
| 11-Oct-2009 | Billy Twelvetrees | England | 15-Nov-1988 | 20(330) |
| 16-Jan-2010 | Dan Cole | England | 09-May-1987 | 22(253) |
| 11-Oct-2009 | Anthony Allen | England | 01-Sep-1986 | 23(40) |
| 11-Oct-2009 | Jordan Crane | England | 03-Jun-1986 | 23(130) |
| 11-Oct-2009 | Tom Croft | England | 07-Nov-1985 | 23(338) |
| 11-Oct-2009 | Lucas Gonzalez Amorosino | Argentina | 02-Nov-1985 | 23(343) |
| 13-Dec-2009 | Toby Flood | England | 08-Aug-1985 | 24(127) |
| 11-Oct-2009 | Johne Murphy | Ireland | 10-Nov-1984 | 24(335) |
| 17-Oct-2009 | Geoffrey Parling | England | 28-Oct-1983 | 25(355) |
| 11-Oct-2009 | Marcos Ayerza | Argentina | 12-Jan-1983 | 26(273) |
| 19-Dec-2009 | Dan Hipkiss | England | 04-Jun-1982 | 27(198) |
| 16-Jan-2010 | Ben Woods | England | 09-Jun-1982 | 27(221) |
| 17-Oct-2009 | Martin Castrogiovanni | Italy | 21-Oct-1981 | 27(361) |
| 16-Jan-2010 | Sam Vesty | England | 26-Nov-1981 | 28(51) |
| 13-Dec-2009 | Alesana Tuilagi | Samoa | 24-Feb-1981 | 28(292) |
| 11-Oct-2009 | Louis Deacon | England | 07-Oct-1980 | 29(4) |
| 13-Dec-2009 | Aaron Mauger | New Zealand | 29-Nov-1980 | 29(14) |
| 17-Oct-2009 | Boris Stankovich | England | 01-Sep-1980 | 29(46) |
| 11-Oct-2009 | James Grindal | England | 18-Aug-1980 | 29(54) |
| 11-Oct-2009 | Jeremy Staunton | Ireland | 07-May-1980 | 29(157) |
| 11-Oct-2009 | Scott Hamilton | New Zealand | 04-Mar-1980 | 29(221) |
| 13-Dec-2009 | Lote Tuqiri | Australia | 23-Sep-1979 | 30(82) |
| 13-Dec-2009 | Craig Newby | New Zealand | 27-Jul-1979 | 30(140) |
| 11-Oct-2009 | Lewis Moody | England | 12-Jun-1978 | 31(122) |
| 11-Oct-2009 | George Chuter | England | 09-Jul-1976 | 33(94) |
| 11-Oct-2009 | Ben Kay | England | 14-Dec-1975 | 33(302) |
| 11-Oct-2009 | Julian White | England | 14-May-1973 | 36(150) |
| 13-Dec-2009 | Mefin Davies | Wales | 02-Sep-1972 | 37(102) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).