| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 16-Jan-2010 |  Paul Couet-Lannes | Biarritz | France | 12-Mar-1991 | 18(311) |
| 16-Jan-2010 | Yann Lesgourgues | Biarritz | France | 01-Jan-1991 | 19(15) |
| 11-Oct-2009 |  James Gaskell | Sale Sharks | England | 20-May-1990 | 19(144) |
| 09-Oct-2009 |  Tom Homer | London Irish | England | 01-Apr-1990 | 19(191) |
| 11-Oct-2009 | Jonny Kennedy | Sale Sharks | Scotland | 16-Feb-1990 | 19(237) |
| 10-Oct-2009 |  Florian Cazenave | Perpignan | France | 30-Dec-1989 | 19(284) |
| 11-Oct-2009 |  Daniel Biggar | Ospreys | Wales | 16-Oct-1989 | 19(360) |
| 16-Jan-2010 |  Dave O'Callaghan | Munster | Ireland | 12-Jan-1990 | 20(4) |
| 24-Jan-2010 |  Rory Clegg | Harlequins | England | 06-Jan-1990 | 20(18) |
| 13-Dec-2009 | George Lowe | Harlequins | England | 22-Oct-1989 | 20(52) |
| 09-Oct-2009 |  Charles Sharples | Gloucester | England | 17-Aug-1989 | 20(53) |
| 19-Dec-2009 |  Ben Youngs | Leicester Tigers | England | 05-Sep-1989 | 20(105) |
| 11-Dec-2009 | Richie Gray | Glasgow Warriors | Scotland | 24-Aug-1989 | 20(109) |
| 11-Oct-2009 | Carl Fearns | Sale Sharks | England | 25-May-1989 | 20(139) |
| 09-Oct-2009 | Jason Tovey | Gwent Dragons | Wales | 28-Apr-1989 | 20(164) |
| 17-Oct-2009 | David Lewis | Gloucester | England | 29-Apr-1989 | 20(171) |
| 18-Oct-2009 |  Alexandre Lapandry | Clermont Auvergne | France | 13-Apr-1989 | 20(188) |
| 24-Jan-2010 | Laurent Tranier | Biarritz | France | 16-Jul-1989 | 20(192) |
| 23-Jan-2010 |  Djibril Camara | Stade Francais | France | 22-Jun-1989 | 20(215) |
| 10-Oct-2009 |  Courtney Lawes | Northampton Saints | England | 23-Feb-1989 | 20(229) |
| 12-Dec-2009 | Guilaume Namy | Brive | France | 03-Apr-1989 | 20(253) |
| 16-Oct-2009 |  Leigh Halfpenny | Cardiff Blues | Wales | 22-Dec-1988 | 20(298) |
| 10-Oct-2009 |  Daniel Evans | Scarlets | Wales | 15-Nov-1988 | 20(329) |
| 10-Oct-2009 | Morgan Parra | Clermont Auvergne | France | 15-Nov-1988 | 20(329) |
| 11-Oct-2009 | Billy Twelvetrees | Leicester Tigers | England | 15-Nov-1988 | 20(330) |
| 10-Oct-2009 | Mickey Wilson | Viadana | Italy | 04-Nov-1988 | 20(340) |
| 16-Oct-2009 | Phil Price | Gwent Dragons | Wales | 10-Nov-1988 | 20(340) |
| 11-Dec-2009 | Yohan Vivalda | Perpignan | France | 09-Dec-1988 | 21(2) |
| 10-Oct-2009 | Sam Warburton | Cardiff Blues | Wales | 05-Oct-1988 | 21(5) |
| 10-Apr-2010 | Wenceslas Lauret | Biarritz | France | 28-Mar-1989 | 21(13) |
| 12-Dec-2009 | Gareth Owen | Ospreys | Wales | 05-Nov-1988 | 21(37) |
| 12-Dec-2009 | Mathieu Bastareaud | Stade Francais | France | 17-Sep-1988 | 21(86) |
| 11-Dec-2009 | Bertrand Guiry | Perpignan | France | 30-Jul-1988 | 21(134) |
| 17-Oct-2009 |  Yann David | Toulouse | France | 15-Apr-1988 | 21(185) |
| 10-Oct-2009 | Jon Davies | Scarlets | Wales | 05-Apr-1988 | 21(188) |
| 15-Jan-2010 |  Jamie Smith | Ulster | Ireland | 25-Jun-1988 | 21(204) |
| 09-Oct-2009 | Dan Lydiatt | Gwent Dragons | Wales | 18-Dec-1987 | 21(296) |
| 15-Jan-2010 | Ruaridh Jackson | Glasgow Warriors | Scotland | 12-Feb-1988 | 21(338) |
| 19-Dec-2009 | Yohan Maestri | Toulouse | France | 14-Jan-1988 | 21(340) |
| 09-Oct-2009 |  Cian Healy | Leinster | Ireland | 07-Oct-1987 | 22(3) |
| 10-Oct-2009 | Keith Earls | Munster | Ireland | 02-Oct-1987 | 22(9) |
| 24-Jan-2010 | Jordan Turner-Hall | Harlequins | England | 05-Jan-1988 | 22(20) |
| 09-Oct-2009 | Luke Fitzgerald | Leinster | Ireland | 13-Sep-1987 | 22(27) |
| 10-Oct-2009 | Lou Reed | Scarlets | Wales | 10-Sep-1987 | 22(31) |
| 17-Oct-2009 | Alexis Palisson | Brive | France | 09-Sep-1987 | 22(39) |
| 13-Dec-2009 | Ilikena Bolakoro | Biarritz | Fiji | 28-Oct-1987 | 22(47) |
| 23-Jan-2010 | Thibault Dubarry | Brive | France | 24-Nov-1987 | 22(61) |
| 10-Oct-2009 | Antoine Burban | Stade Francais | France | 22-Jul-1987 | 22(81) |
| 09-Oct-2009 | Timoci Nagusa | Ulster | Fiji | 14-Jul-1987 | 22(88) |
| 10-Oct-2009 | Richard Vernon | Glasgow Warriors | Scotland | 07-Jul-1987 | 22(96) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).