| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 10-Oct-2009 |  Oliver Williams | Luctonians | England | 27-Dec-1993 | 15(287) |
| 31-Oct-2009 | Sam Egerton | Newport (Salop) | England | 15-Jan-1992 | 17(290) |
| 03-Oct-2009 | Drew Cheshire | Luctonians | England | 06-Sep-1991 | 18(28) |
| 12-Sep-2009 | William Hodnett | Luctonians | England | 28-Jun-1991 | 18(77) |
| 05-Sep-2009 | Craig Hossack | Luctonians | England | 23-Feb-1991 | 18(195) |
| 05-Sep-2009 | Daniel Smith | Luctonians | England | 01-Oct-1990 | 18(340) |
| 05-Sep-2009 | Mark Kohler | Luton | England | 16-Aug-1989 | 20(20) |
| 07-Nov-2009 | Alec Smith | Hinckley | England | 13-Jul-1989 | 20(117) |
| 06-Feb-2010 | Alexander James | Luctonians | England | 04-Feb-1989 | 21(2) |
| 23-Jan-2010 | Alexandru Mitu | Luctonians | Romania | 03-Sep-1988 | 21(142) |
| 05-Sep-2009 | Ben James | Luctonians | England | 20-Feb-1988 | 21(198) |
| 12-Sep-2009 | Adam Knight | Hereford | England | 25-Jul-1987 | 22(50) |
| 05-Sep-2009 | Adam Stevens | Newport (Salop) | England | 01-Jul-1987 | 22(67) |
| 05-Sep-2009 | Tomasi Tanumi | Newport (Salop) | Other | 18-May-1987 | 22(111) |
| 24-Oct-2009 | Nigel Burrows | Bromsgrove | England | 01-Jul-1987 | 22(116) |
| 05-Sep-2009 | Terry Cooper | Luton | Other | 01-Dec-1986 | 22(279) |
| 10-Oct-2009 | Ben Brierley | Ampthill | Germany | 20-Dec-1986 | 22(295) |
| 10-Oct-2009 | Michael Jones | Luctonians | England | 18-Oct-1986 | 22(358) |
| 16-Jan-2010 | Guy Thompson | Hereford | England | 23-Jan-1987 | 22(359) |
| 05-Sep-2009 | Chris Harris | Kettering | England | 29-Jun-1986 | 23(68) |
| 31-Oct-2009 | Scott Rawlings | Longton | England | 24-Jun-1986 | 23(129) |
| 05-Sep-2009 | Thomas Dines | Luctonians | Other | 08-Jan-1986 | 23(240) |
| 05-Sep-2009 | Laurence Alden | Luton | England | 15-Feb-1985 | 24(202) |
| 05-Sep-2009 | Brett Prior | Luctonians | England | 29-Nov-1984 | 24(280) |
| 05-Sep-2009 | Ben Forbes | Ampthill | Other | 27-Sep-1984 | 24(343) |
| 05-Sep-2009 | Nicholas Stevens | Luton | England | 23-Aug-1984 | 25(13) |
| 05-Sep-2009 | Liam Lancaster | Luton | Other | 19-Aug-1983 | 26(18) |
| 05-Sep-2009 | Mike Rust | Kenilworth | Other | 29-Jun-1983 | 26(69) |
| 05-Sep-2009 | Ade Hales | Kenilworth | Other | 25-May-1983 | 26(104) |
| 31-Oct-2009 | Gareth Rawlings | Longton | England | 02-Jun-1983 | 26(152) |
| 26-Sep-2009 | William Temperley | Luton | England | 16-Sep-1982 | 27(10) |
| 05-Sep-2009 | James Hersey | Kenilworth | England | 10-Aug-1982 | 27(26) |
| 07-Nov-2009 | Scott Hills | Luton | Other | 19-Sep-1982 | 27(49) |
| 05-Sep-2009 | Christoff Blom | Bedford Athletic | South Africa | 30-Mar-1982 | 27(159) |
| 05-Sep-2009 | Mike Ormesher | Luton | England | 28-Jan-1982 | 27(220) |
| 05-Sep-2009 | Chris Jaques | Luctonians | Other | 22-Jan-1982 | 27(226) |
| 05-Sep-2009 | Andy Phillips | Peterborough | England | 04-Nov-1981 | 27(305) |
| 05-Sep-2009 | Sam Overton | Kenilworth | Other | 18-Jul-1981 | 28(49) |
| 31-Oct-2009 | James Aston | Malvern | Other | 19-Jan-1981 | 28(285) |
| 05-Sep-2009 | Lee Anker | Luton | England | 01-Nov-1980 | 28(308) |
| 05-Sep-2009 | Gareth Jones | South Leicester | Other | 27-Aug-1980 | 29(9) |
| 06-Feb-2010 | James Wadey | Kenilworth | England | 08-Jan-1981 | 29(29) |
| 05-Sep-2009 | Nicholas Byron | Luctonians | England | 23-Mar-1980 | 29(166) |
| 05-Sep-2009 | Tristan Wati | Kenilworth | Other | 11-Feb-1980 | 29(207) |
| 10-Oct-2009 | Stephen Jaques | Luctonians | England | 16-Jan-1980 | 29(268) |
| 05-Sep-2009 | Tom Beechy | Malvern | England | 14-Oct-1979 | 29(327) |
| 31-Oct-2009 | Sam Viggers | Kenilworth | Other | 19-Mar-1979 | 30(227) |
| 05-Sep-2009 | Anthony Marfell | Luctonians | England | 16-Oct-1978 | 30(325) |
| 26-Sep-2009 | Will Price | Luctonians | England | 12-Aug-1978 | 31(46) |
| 05-Sep-2009 | Toby Wilson | Bromsgrove | Other | 11-Sep-1977 | 31(360) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).