| MD | Players | Nationality | DOB | A(D) |
|---|---|---|---|---|
| 31-Oct-2009 | Callum Green | England | 15-Jul-1990 | 19(108) |
| 04-Sep-2009 | Ben Youngs | England | 05-Sep-1989 | 19(364) |
| 14-Feb-2010 | Billy Twelvetrees | England | 15-Nov-1988 | 21(91) |
| 20-Nov-2009 | Dan Cole | England | 09-May-1987 | 22(196) |
| 04-Sep-2009 | Anthony Allen | England | 01-Sep-1986 | 23(3) |
| 31-Oct-2009 | Ben Pienaar | England | 10-Sep-1986 | 23(51) |
| 04-Sep-2009 | Jordan Crane | England | 03-Jun-1986 | 23(93) |
| 04-Sep-2009 | Matt Smith | England | 15-Nov-1985 | 23(293) |
| 12-Sep-2009 | Tom Croft | England | 07-Nov-1985 | 23(309) |
| 24-Oct-2009 | Lucas Gonzalez Amorosino | Argentina | 02-Nov-1985 | 23(356) |
| 28-Nov-2009 | Toby Flood | England | 08-Aug-1985 | 24(112) |
| 04-Sep-2009 | Richard Blaze | England | 19-Apr-1985 | 24(138) |
| 12-Sep-2009 | Johne Murphy | Ireland | 10-Nov-1984 | 24(306) |
| 31-Oct-2009 | Geoffrey Parling | England | 28-Oct-1983 | 26(4) |
| 04-Sep-2009 | Marcos Ayerza | Argentina | 12-Jan-1983 | 26(236) |
| 04-Sep-2009 | Ben Woods | England | 09-Jun-1982 | 27(87) |
| 04-Sep-2009 | Dan Hipkiss | England | 04-Jun-1982 | 27(92) |
| 03-Oct-2009 | Joe Duffey | New Zealand | 22-Jun-1982 | 27(103) |
| 12-Sep-2009 | Harry Ellis | England | 17-May-1982 | 27(118) |
| 19-Sep-2009 | Brett Deacon | England | 07-Mar-1982 | 27(196) |
| 04-Sep-2009 | Martin Castrogiovanni | Italy | 21-Oct-1981 | 27(318) |
| 14-Feb-2010 | Robert Lee Harris | South Africa | 30-Mar-1982 | 27(321) |
| 26-Sep-2009 | Aaron Mauger | New Zealand | 29-Nov-1980 | 28(301) |
| 04-Sep-2009 | Louis Deacon | England | 07-Oct-1980 | 28(332) |
| 14-Feb-2010 | Alesana Tuilagi | Samoa | 24-Feb-1981 | 28(355) |
| 03-Oct-2009 | Boris Stankovich | England | 01-Sep-1980 | 29(32) |
| 26-Sep-2009 | James Grindal | England | 18-Aug-1980 | 29(39) |
| 04-Sep-2009 | Jeremy Staunton | Ireland | 07-May-1980 | 29(120) |
| 04-Sep-2009 | Scott Hamilton | New Zealand | 04-Mar-1980 | 29(184) |
| 04-Sep-2009 | Craig Newby | New Zealand | 27-Jul-1979 | 30(40) |
| 20-Nov-2009 | Lote Tuqiri | Australia | 23-Sep-1979 | 30(59) |
| 26-Sep-2009 | Lewis Moody | England | 12-Jun-1978 | 31(107) |
| 04-Sep-2009 | Geordan Murphy | Ireland | 19-Apr-1978 | 31(138) |
| 04-Sep-2009 | George Chuter | England | 09-Jul-1976 | 33(57) |
| 19-Sep-2009 | Ben Kay | England | 14-Dec-1975 | 33(280) |
| 26-Sep-2009 | Julian White | England | 14-May-1973 | 36(135) |
| 19-Sep-2009 | Mefin Davies | Wales | 02-Sep-1972 | 37(17) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).