| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 28-Nov-2008 |  Kristian Phillips | Ospreys | Wales | 02-Sep-1990 | 18(88) |
| 28-Nov-2008 | Matthew Jarvis | Ospreys | Wales | 25-Aug-1990 | 18(96) |
| 12-Sep-2008 | Daniel Biggar | Ospreys | Wales | 16-Oct-1989 | 18(332) |
| 15-May-2009 | Ashley Beck | Ospreys | Wales | 15-Apr-1990 | 19(30) |
| 24-Apr-2009 |  James Loxton | Cardiff Blues | Wales | 02-Feb-1990 | 19(81) |
| 16-May-2009 |  Ian McKinley | Leinster | Italy | 04-Dec-1989 | 19(163) |
| 19-Dec-2008 | Jason Tovey | Gwent Dragons | Wales | 28-Apr-1989 | 19(235) |
| 15-May-2009 | Richie Gray | Glasgow Warriors | Scotland | 24-Aug-1989 | 19(264) |
| 12-Sep-2008 | Leigh Halfpenny | Cardiff Blues | Wales | 22-Dec-1988 | 19(264) |
| 05-Sep-2008 | Rhys Webb | Ospreys | Wales | 09-Dec-1988 | 19(270) |
| 12-Sep-2008 | Gareth Owen | Ospreys | Wales | 05-Nov-1988 | 19(311) |
| 16-May-2009 |  Nick Reynolds | Scarlets | Wales | 07-Apr-1989 | 20(39) |
| 08-Mar-2009 | Daniel Evans | Scarlets | Wales | 15-Nov-1988 | 20(113) |
| 19-Sep-2008 | Jon Davies | Scarlets | Wales | 05-Apr-1988 | 20(167) |
| 03-Apr-2009 | Sam Warburton | Cardiff Blues | Wales | 05-Oct-1988 | 20(180) |
| 15-May-2009 | David Evans | Ospreys | Wales | 19-Aug-1988 | 20(269) |
| 14-Sep-2008 | Dan Lydiatt | Gwent Dragons | Wales | 18-Dec-1987 | 20(271) |
| 09-Sep-2008 | Darren Allinson | Cardiff Blues | Wales | 13-Nov-1987 | 20(301) |
| 06-Sep-2008 | Cian Healy | Leinster | Ireland | 07-Oct-1987 | 20(335) |
| 05-Sep-2008 |  Keith Earls | Munster | Ireland | 02-Oct-1987 | 20(339) |
| 05-Sep-2008 | Lou Reed | Scarlets | Wales | 10-Sep-1987 | 20(361) |
| 12-Sep-2008 | Luke Fitzgerald | Leinster | Ireland | 13-Sep-1987 | 20(365) |
| 20-Feb-2009 | Ruaridh Jackson | Glasgow Warriors | Scotland | 12-Feb-1988 | 21(9) |
| 19-Dec-2008 | Robert Lewis | Gwent Dragons | Wales | 20-Nov-1987 | 21(30) |
| 05-Sep-2008 |  Timoci Nagusa | Ulster | Fiji | 14-Jul-1987 | 21(54) |
| 05-Sep-2008 | Lewis Evans | Gwent Dragons | Wales | 09-Jul-1987 | 21(59) |
| 12-Sep-2008 | Niall O'Connor | Ulster | Ireland | 07-Jul-1987 | 21(68) |
| 14-Sep-2008 | Huw Gustafson | Gwent Dragons | Wales | 08-Jul-1987 | 21(69) |
| 28-Nov-2008 | Mark McCrea | Ulster | Ireland | 07-Sep-1987 | 21(83) |
| 13-Sep-2008 | Aidan Wynne | Connacht | Ireland | 26-May-1987 | 21(111) |
| 05-Sep-2008 | Darren Cave | Ulster | Ireland | 05-Apr-1987 | 21(154) |
| 05-Sep-2008 | Ian Keatley | Connacht | Ireland | 01-Apr-1987 | 21(158) |
| 12-Sep-2008 | David Pollock | Ulster | Ireland | 19-Mar-1987 | 21(178) |
| 21-Feb-2009 | Paul O'Donohoe | Leinster | Ireland | 27-Aug-1987 | 21(179) |
| 05-Sep-2008 | Ashley Smith | Gwent Dragons | Wales | 13-Feb-1987 | 21(205) |
| 19-Sep-2008 | Sean O'Brien | Leinster | Ireland | 14-Feb-1987 | 21(218) |
| 16-May-2009 | Kyle Tonetti | Leinster | Ireland | 08-Oct-1987 | 21(221) |
| 28-Nov-2008 | Tom James | Cardiff Blues | Wales | 17-Apr-1987 | 21(226) |
| 26-Sep-2008 | Andrew Browne | Connacht | Ireland | 21-Jan-1987 | 21(249) |
| 19-Sep-2008 | Ken Owens | Scarlets | Wales | 03-Jan-1987 | 21(260) |
| 07-Mar-2009 | Ian Whitten | Ulster | Ireland | 05-Jun-1987 | 21(276) |
| 06-Sep-2008 | Jamie Roberts | Cardiff Blues | Wales | 08-Nov-1986 | 21(303) |
| 22-Feb-2009 | Martyn Thomas | Gwent Dragons | Wales | 24-Apr-1987 | 21(305) |
| 28-Nov-2008 | Rhys Priestland | Scarlets | Wales | 07-Jan-1987 | 21(326) |
| 05-Sep-2008 | John Barclay | Glasgow Warriors | Scotland | 24-Sep-1986 | 21(347) |
| 05-Sep-2008 | Darren Daniels | Scarlets | Wales | 13-Sep-1986 | 21(358) |
| 15-May-2009 | Pat McArthur | Glasgow Warriors | Scotland | 27-Apr-1987 | 22(19) |
| 09-Sep-2008 | Aled Brew | Cardiff Blues | Wales | 09-Aug-1986 | 22(32) |
| 28-Nov-2008 | Callum Forrester | Glasgow Warriors | Scotland | 03-Oct-1986 | 22(57) |
| 05-Sep-2008 | Rory Sidey | Gwent Dragons | Australia | 04-Jul-1986 | 22(64) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).