| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 04-Oct-2008 |  Rob Milligan | Northampton Saints | England | 17-Apr-1990 | 18(171) |
| 03-Oct-2008 |  Will Welch | Newcastle Falcons | England | 03-Apr-1990 | 18(184) |
| 03-Oct-2008 | Rory Clegg | Newcastle Falcons | England | 06-Jan-1990 | 18(271) |
| 31-Oct-2008 |  Ed Styles | Sale Sharks | England | 07-Dec-1989 | 18(329) |
| 05-Oct-2008 |  Graham Kitchener | Worcester Warriors | England | 29-Sep-1989 | 19(6) |
| 04-Oct-2008 |  Ben Youngs | Leicester Tigers | England | 05-Sep-1989 | 19(29) |
| 04-Oct-2008 |  Josh Ovens | Bath | England | 11-Jul-1989 | 19(85) |
| 02-Nov-2008 | Robert Miller | Newcastle Falcons | England | 05-Aug-1989 | 19(89) |
| 04-Oct-2008 | Dan Hemingway | Leicester Tigers | England | 03-Jun-1989 | 19(123) |
| 04-Oct-2008 | Carl Fearns | Sale Sharks | England | 25-May-1989 | 19(132) |
| 03-Oct-2008 | Jason Tovey | Gwent Dragons | Wales | 28-Apr-1989 | 19(158) |
| 04-Oct-2008 |  Luke Eves | Bristol Bears | England | 11-Apr-1989 | 19(176) |
| 25-Oct-2008 |  Sebastian Stegmann | Harlequins | England | 12-Apr-1989 | 19(196) |
| 04-Oct-2008 | Courtney Lawes | Northampton Saints | England | 23-Feb-1989 | 19(223) |
| 03-Oct-2008 | Michael Young | Newcastle Falcons | England | 31-Dec-1988 | 19(276) |
| 04-Oct-2008 |  Leigh Halfpenny | Cardiff Blues | Wales | 22-Dec-1988 | 19(286) |
| 05-Oct-2008 | Rhys Webb | Ospreys | Wales | 09-Dec-1988 | 19(300) |
| 04-Oct-2008 | Ed Jackson | Bath | England | 02-Dec-1988 | 19(306) |
| 05-Oct-2008 |  Jon Fisher | London Irish | England | 15-Sep-1988 | 20(20) |
| 05-Oct-2008 |  Andy Saull | Saracens | England | 27-Aug-1988 | 20(39) |
| 05-Oct-2008 | Harry Spencer | Saracens | England | 12-Jul-1988 | 20(85) |
| 05-Oct-2008 | Miles Benjamin | Worcester Warriors | England | 11-May-1988 | 20(147) |
| 05-Oct-2008 | Alex Goode | Saracens | England | 07-May-1988 | 20(151) |
| 05-Oct-2008 | Charles Fellows | Worcester Warriors | England | 26-Apr-1988 | 20(162) |
| 05-Oct-2008 | Noah Cato | Saracens | England | 31-Mar-1988 | 20(188) |
| 04-Oct-2008 |  Mark Odejobi | London Wasps | England | 09-Mar-1988 | 20(209) |
| 05-Oct-2008 | Matthew Cox | Worcester Warriors | England | 06-Mar-1988 | 20(213) |
| 25-Oct-2008 | Gareth Williams | Harlequins | Wales | 07-Mar-1988 | 20(232) |
| 05-Oct-2008 | Jacob Abbott | Worcester Warriors | England | 04-Feb-1988 | 20(244) |
| 04-Oct-2008 | Mathew Turner | Bristol Bears | England | 18-Jan-1988 | 20(260) |
| 03-Oct-2008 | Dan Lydiatt | Gwent Dragons | Wales | 18-Dec-1987 | 20(290) |
| 02-Nov-2008 | Jordan Turner-Hall | Harlequins | England | 05-Jan-1988 | 20(302) |
| 25-Oct-2008 | Seb Jewell | Harlequins | England | 20-Dec-1987 | 20(310) |
| 05-Oct-2008 | Alex Grove | Worcester Warriors | Scotland | 30-Nov-1987 | 20(310) |
| 25-Oct-2008 | Tom Parker | London Irish | England | 19-Dec-1987 | 20(311) |
| 03-Oct-2008 | Robert Lewis | Gwent Dragons | Wales | 20-Nov-1987 | 20(318) |
| 04-Oct-2008 | Chris Ashwin | Bristol Bears | England | 19-Nov-1987 | 20(320) |
| 24-Oct-2008 | Danny Cipriani | London Wasps | England | 02-Nov-1987 | 20(357) |
| 04-Oct-2008 | Lachlan Mitchell | London Wasps | Australia | 30-Sep-1987 | 21(5) |
| 04-Oct-2008 | Dominic Waldouck | London Wasps | England | 26-Sep-1987 | 21(9) |
| 25-Oct-2008 | Charlie Gower | London Irish | England | 28-Sep-1987 | 21(28) |
| 05-Oct-2008 | Adam Thompstone | London Irish | England | 04-Sep-1987 | 21(32) |
| 24-Oct-2008 | Jack Cuthbert | Bath | Scotland | 03-Sep-1987 | 21(52) |
| 03-Oct-2008 | Lewis Evans | Gwent Dragons | Wales | 09-Jul-1987 | 21(87) |
| 03-Oct-2008 | Huw Gustafson | Gwent Dragons | Wales | 08-Jul-1987 | 21(88) |
| 04-Oct-2008 | David Tait | Sale Sharks | England | 05-Jul-1987 | 21(92) |
| 01-Nov-2008 | Tom Arscott | Bristol Bears | England | 25-Jul-1987 | 21(100) |
| 05-Oct-2008 | Tom Mercey | Saracens | England | 15-Jun-1987 | 21(113) |
| 04-Oct-2008 | Dan Cole | Leicester Tigers | England | 09-May-1987 | 21(149) |
| 04-Oct-2008 | Selorm Kuadey | Sale Sharks | England | 05-May-1987 | 21(153) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).