| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 18-Oct-2008 |  Daniel Biggar | Ospreys | Wales | 16-Oct-1989 | 19(2) |
| 17-Jan-2009 |  Ben Youngs | Leicester Tigers | England | 05-Sep-1989 | 19(134) |
| 06-Dec-2008 |  Djibril Camara | Stade Francais | France | 22-Jun-1989 | 19(167) |
| 17-Jan-2009 |  Laurent Tranier | Biarritz | France | 16-Jul-1989 | 19(185) |
| 05-Dec-2008 |  Carl Fearns | Sale Sharks | England | 25-May-1989 | 19(194) |
| 13-Dec-2008 | Jason Tovey | Gwent Dragons | Wales | 28-Apr-1989 | 19(229) |
| 07-Dec-2008 |  Alexandre Lapandry | Clermont Auvergne | France | 13-Apr-1989 | 19(238) |
| 13-Dec-2008 | Wenceslas Lauret | Biarritz | France | 28-Mar-1989 | 19(260) |
| 11-Oct-2008 |  Leigh Halfpenny | Cardiff Blues | Wales | 22-Dec-1988 | 19(293) |
| 18-Oct-2008 | Rhys Webb | Ospreys | Wales | 09-Dec-1988 | 19(313) |
| 11-Oct-2008 | Andrea Pratichetti | Calvisano | Italy | 26-Nov-1988 | 19(319) |
| 06-Dec-2008 | Mathieu Bastareaud | Stade Francais | France | 17-Sep-1988 | 20(80) |
| 25-Jan-2009 | Paul Bonnefond | Castres | France | 13-Sep-1988 | 20(134) |
| 05-Dec-2008 |  Jon Davies | Scarlets | Wales | 05-Apr-1988 | 20(244) |
| 11-Oct-2008 |  Jordan Turner-Hall | Harlequins | England | 05-Jan-1988 | 20(280) |
| 07-Dec-2008 | Ruaridh Jackson | Glasgow Warriors | Scotland | 12-Feb-1988 | 20(299) |
| 12-Oct-2008 |  Danny Cipriani | London Wasps | England | 02-Nov-1987 | 20(345) |
| 10-Oct-2008 |  Keith Earls | Munster | Ireland | 02-Oct-1987 | 21(9) |
| 11-Oct-2008 |  Luke Fitzgerald | Leinster | Ireland | 13-Sep-1987 | 21(29) |
| 05-Dec-2008 | Ilikena Bolakoro | Biarritz | Fiji | 28-Oct-1987 | 21(39) |
| 25-Jan-2009 | Dan Lydiatt | Gwent Dragons | Wales | 18-Dec-1987 | 21(39) |
| 12-Dec-2008 | Cian Healy | Leinster | Ireland | 07-Oct-1987 | 21(67) |
| 05-Dec-2008 | Dominic Waldouck | London Wasps | England | 26-Sep-1987 | 21(71) |
| 11-Oct-2008 | Lewis Evans | Gwent Dragons | Wales | 09-Jul-1987 | 21(95) |
| 11-Oct-2008 |  Niall O'Connor | Ulster | Ireland | 07-Jul-1987 | 21(97) |
| 18-Oct-2008 | Timoci Nagusa | Ulster | Fiji | 14-Jul-1987 | 21(97) |
| 17-Oct-2008 | Richard Vernon | Glasgow Warriors | Scotland | 07-Jul-1987 | 21(103) |
| 11-Oct-2008 | Luke McLean | Calvisano | Italy | 11-May-1987 | 21(154) |
| 11-Oct-2008 | Darren Cave | Ulster | Ireland | 05-Apr-1987 | 21(190) |
| 24-Jan-2009 | David Tait | Sale Sharks | England | 05-Jul-1987 | 21(204) |
| 18-Oct-2008 | David Pollock | Ulster | Ireland | 19-Mar-1987 | 21(214) |
| 24-Jan-2009 | Ian Whitten | Ulster | Ireland | 05-Jun-1987 | 21(234) |
| 05-Dec-2008 | Marc Jones | Sale Sharks | Wales | 03-Apr-1987 | 21(247) |
| 11-Oct-2008 | David Doherty | Sale Sharks | England | 28-Jan-1987 | 21(257) |
| 18-Jan-2009 | Tom James | Cardiff Blues | Wales | 17-Apr-1987 | 21(277) |
| 11-Oct-2008 | Danny Care | Harlequins | England | 02-Jan-1987 | 21(283) |
| 12-Oct-2008 | Matt Banahan | Bath | England | 30-Dec-1986 | 21(287) |
| 06-Dec-2008 | Luca Beccaris | Calvisano | Italy | 19-Feb-1987 | 21(291) |
| 06-Dec-2008 | Sean O'Brien | Leinster | Ireland | 14-Feb-1987 | 21(296) |
| 13-Dec-2008 | Ashley Smith | Gwent Dragons | Wales | 13-Feb-1987 | 21(304) |
| 23-Jan-2009 | Filippo Cristiano | Calvisano | Italy | 13-Mar-1987 | 21(317) |
| 12-Oct-2008 |  Maxime Medard | Toulouse | France | 16-Nov-1986 | 21(331) |
| 12-Dec-2008 | Rhys Priestland | Scarlets | Wales | 07-Jan-1987 | 21(340) |
| 24-Jan-2009 | Thomas Combezou | Clermont Auvergne | France | 26-Jan-1987 | 21(364) |
| 11-Oct-2008 | John Barclay | Glasgow Warriors | Scotland | 24-Sep-1986 | 22(18) |
| 05-Dec-2008 | Laurent Delboulbes | Montauban | France | 17-Nov-1986 | 22(19) |
| 11-Oct-2008 | Darren Daniels | Scarlets | Wales | 13-Sep-1986 | 22(29) |
| 12-Oct-2008 | Nick Abendanon | Bath | England | 27-Aug-1986 | 22(47) |
| 18-Jan-2009 | Jamie Roberts | Cardiff Blues | Wales | 08-Nov-1986 | 22(72) |
| 18-Jan-2009 | Lee Williams | Scarlets | Wales | 27-Oct-1986 | 22(84) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).