| MD | Players | Nationality | DOB | A(D) |
|---|---|---|---|---|
| 26-Sep-2008 | Dan Cole | England | 09-May-1987 | 21(141) |
| 07-Sep-2008 | Jordan Crane | England | 03-Jun-1986 | 22(97) |
| 07-Mar-2009 | Ben Pienaar | England | 10-Sep-1986 | 22(179) |
| 07-Sep-2008 | Matt Smith | England | 15-Nov-1985 | 22(297) |
| 07-Sep-2008 | Tom Croft | England | 07-Nov-1985 | 22(305) |
| 07-Sep-2008 | Tom Varndell | England | 16-Aug-1985 | 23(22) |
| 07-Sep-2008 | Toby Flood | England | 08-Aug-1985 | 23(30) |
| 07-Sep-2008 | Richard Blaze | England | 19-Apr-1985 | 23(141) |
| 20-Sep-2008 | Johne Murphy | Ireland | 10-Nov-1984 | 23(314) |
| 20-Sep-2008 | Benjamin Kayser | France | 26-Jul-1984 | 24(56) |
| 20-Sep-2008 | Julian Dupuy | France | 19-Dec-1983 | 24(276) |
| 07-Sep-2008 | Marcos Ayerza | Argentina | 12-Jan-1983 | 25(239) |
| 15-Nov-2008 | Derick Hougaard | South Africa | 04-Jan-1983 | 25(316) |
| 07-Sep-2008 | Dan Hipkiss | England | 04-Jun-1982 | 26(96) |
| 26-Sep-2008 | Ben Woods | England | 09-Jun-1982 | 26(110) |
| 07-Sep-2008 | Harry Ellis | England | 17-May-1982 | 26(114) |
| 22-Nov-2008 | Brett Deacon | England | 07-Mar-1982 | 26(261) |
| 15-Feb-2009 | Sam Vesty | England | 26-Nov-1981 | 27(81) |
| 21-Feb-2009 | Martin Castrogiovanni | Italy | 21-Oct-1981 | 27(123) |
| 07-Sep-2008 | Alesana Tuilagi | Samoa | 24-Feb-1981 | 27(195) |
| 13-Sep-2008 | Aaron Mauger | New Zealand | 29-Nov-1980 | 27(288) |
| 01-Oct-2008 | Boris Stankovich | England | 01-Sep-1980 | 28(30) |
| 15-Feb-2009 | Louis Deacon | England | 07-Oct-1980 | 28(131) |
| 07-Sep-2008 | Ben Herring | New Zealand | 14-Mar-1980 | 28(177) |
| 22-Nov-2008 | Scott Hamilton | New Zealand | 04-Mar-1980 | 28(263) |
| 27-Mar-2009 | Ayoola Erinle | England | 20-Feb-1980 | 29(36) |
| 26-Sep-2008 | Marco Wentzel | South Africa | 06-May-1979 | 29(144) |
| 20-Dec-2008 | Craig Newby | New Zealand | 27-Jul-1979 | 29(147) |
| 28-Nov-2008 | Seru Rabeni | Fiji | 27-Dec-1978 | 29(337) |
| 07-Sep-2008 | Geordan Murphy | Ireland | 19-Apr-1978 | 30(142) |
| 15-Nov-2008 | Lewis Moody | England | 12-Jun-1978 | 30(157) |
| 07-Sep-2008 | George Chuter | England | 09-Jul-1976 | 32(60) |
| 07-Sep-2008 | Ben Kay | England | 14-Dec-1975 | 32(268) |
| 20-Sep-2008 | Martin Corry | England | 12-Oct-1973 | 34(344) |
| 07-Sep-2008 | Julian White | England | 14-May-1973 | 35(117) |
| 01-Mar-2009 | Mefin Davies | Wales | 02-Sep-1972 | 36(180) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).