| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 05-May-2007 |  Anthony Allen | Gloucester | England | 01-Sep-1986 | 20(246) |
| 05-May-2007 | Ryan Lamb | Gloucester | England | 18-May-1986 | 20(352) |
| 05-May-2007 |  Tom Varndell | Leicester Tigers | England | 16-Aug-1985 | 21(262) |
| 05-May-2007 |  Luke Arscott | Bristol Bears | England | 07-Jul-1984 | 22(302) |
| 05-May-2007 |  Ben Skirving | Saracens | England | 09-Oct-1983 | 23(208) |
| 05-May-2007 | Luke Narraway | Gloucester | England | 07-Sep-1983 | 23(240) |
| 05-May-2007 | Mark Foster | Gloucester | England | 02-Sep-1983 | 23(245) |
| 05-May-2007 | Danny Gray | Bristol Bears | England | 21-May-1983 | 23(349) |
| 05-May-2007 | Marcos Ayerza | Leicester Tigers | Argentina | 12-Jan-1983 | 24(113) |
| 05-May-2007 | Nick Wood | Gloucester | England | 09-Jan-1983 | 24(116) |
| 05-May-2007 | Kameli Ratuvou | Saracens | Fiji | 06-Nov-1982 | 24(180) |
| 12-May-2007 | Ollie Smith | Leicester Tigers | England | 14-Aug-1982 | 24(271) |
| 05-May-2007 | Dan Hipkiss | Leicester Tigers | England | 04-Jun-1982 | 24(335) |
| 05-May-2007 | James Simpson-Daniel | Gloucester | England | 30-May-1982 | 24(340) |
| 05-May-2007 | Harry Ellis | Leicester Tigers | England | 17-May-1982 | 24(353) |
| 05-May-2007 | David Lemi | Bristol Bears | Samoa | 10-Feb-1982 | 25(84) |
| 05-May-2007 | Sam Vesty | Leicester Tigers | England | 26-Nov-1981 | 25(160) |
| 12-May-2007 | Frank Murphy | Leicester Tigers | Ireland | 02-Dec-1981 | 25(161) |
| 05-May-2007 | Shane Jennings | Leicester Tigers | Ireland | 08-Jul-1981 | 25(301) |
| 12-May-2007 | Alesana Tuilagi | Leicester Tigers | Samoa | 24-Feb-1981 | 26(77) |
| 05-May-2007 | Alfie To'oala | Bristol Bears | Samoa | 30-Jan-1981 | 26(95) |
| 05-May-2007 | Lee Robinson | Bristol Bears | England | 30-Dec-1980 | 26(126) |
| 05-May-2007 | Sam Cox | Bristol Bears | England | 11-Dec-1980 | 26(145) |
| 05-May-2007 | Louis Deacon | Leicester Tigers | England | 07-Oct-1980 | 26(210) |
| 05-May-2007 | Marco Bortolami | Gloucester | Italy | 12-Jun-1980 | 26(327) |
| 05-May-2007 | Andy Goode | Leicester Tigers | England | 03-Apr-1980 | 27(31) |
| 05-May-2007 | Joe El Abd | Bristol Bears | England | 23-Feb-1980 | 27(71) |
| 05-May-2007 | Alex Brown | Gloucester | England | 17-May-1979 | 27(353) |
| 05-May-2007 | Iain Balshaw | Gloucester | England | 18-Apr-1979 | 28(17) |
| 05-May-2007 | Neil Brew | Bristol Bears | New Zealand | 09-Mar-1979 | 28(57) |
| 05-May-2007 | Sean Hohneck | Bristol Bears | New Zealand | 08-Jan-1979 | 28(117) |
| 05-May-2007 | Seru Rabeni | Leicester Tigers | Fiji | 27-Dec-1978 | 28(129) |
| 05-May-2007 | Neil de Koch | Saracens | South Africa | 20-Nov-1978 | 28(166) |
| 05-May-2007 | Peter Buxton | Gloucester | England | 21-Aug-1978 | 28(257) |
| 05-May-2007 | Lewis Moody | Leicester Tigers | England | 12-Jun-1978 | 28(327) |
| 05-May-2007 | Willie Walker | Gloucester | New Zealand | 19-May-1978 | 28(351) |
| 05-May-2007 | Shaun Perry | Bristol Bears | England | 04-May-1978 | 29(1) |
| 05-May-2007 | Andrew Hazell | Gloucester | England | 25-Apr-1978 | 29(10) |
| 05-May-2007 | Geordan Murphy | Leicester Tigers | Ireland | 19-Apr-1978 | 29(16) |
| 05-May-2007 | Peter Richards | Gloucester | England | 10-Mar-1978 | 29(56) |
| 05-May-2007 | Dan Scarbrough | Saracens | England | 16-Feb-1978 | 29(78) |
| 05-May-2007 | Leo Cullen | Leicester Tigers | Ireland | 09-Jan-1978 | 29(116) |
| 05-May-2007 | Kevin Sorrell | Saracens | England | 06-Mar-1977 | 30(60) |
| 12-May-2007 | William James | Gloucester | Wales | 22-Dec-1976 | 30(141) |
| 05-May-2007 | Nick Lloyd | Saracens | England | 12-Oct-1976 | 30(205) |
| 05-May-2007 | George Chuter | Leicester Tigers | England | 09-Jul-1976 | 30(300) |
| 05-May-2007 | Carlos Nieto | Gloucester | Italy | 28-Apr-1976 | 31(7) |
| 05-May-2007 | Iain Fullerton | Saracens | Scotland | 25-Apr-1976 | 31(9) |
| 05-May-2007 | Paul Gustard | Saracens | England | 02-Feb-1976 | 31(93) |
| 12-May-2007 | Ben Kay | Leicester Tigers | England | 14-Dec-1975 | 31(149) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).