| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 14-May-2006 |  Tom Varndell | Leicester Tigers | England | 16-Aug-1985 | 20(271) |
| 14-May-2006 |  Topsy Ojo | London Irish | England | 28-Jul-1985 | 20(290) |
| 14-May-2006 | Delon Armitage | London Irish | England | 15-Dec-1983 | 22(151) |
| 14-May-2006 |  Magnus Lund | Sale Sharks | England | 25-Jun-1983 | 22(324) |
| 14-May-2006 | Juan Manuel Leguizamon | London Irish | Argentina | 06-Jun-1983 | 22(343) |
| 27-May-2006 | Richard Wigglesworth | Sale Sharks | England | 09-Jun-1983 | 22(353) |
| 14-May-2006 | Sailosi Tagicakibau | London Irish | Samoa | 14-Nov-1982 | 23(181) |
| 14-May-2006 |  Daniel Leo | London Wasps | Samoa | 02-Oct-1982 | 23(224) |
| 14-May-2006 | Ollie Smith | Leicester Tigers | England | 14-Aug-1982 | 23(273) |
| 14-May-2006 | Harry Ellis | Leicester Tigers | England | 17-May-1982 | 23(362) |
| 14-May-2006 | Paul Hodgson | London Irish | England | 25-Apr-1982 | 24(19) |
| 14-May-2006 | Shane Jennings | Leicester Tigers | Ireland | 08-Jul-1981 | 24(310) |
| 14-May-2006 | Alesana Tuilagi | Leicester Tigers | Samoa | 24-Feb-1981 | 25(79) |
| 14-May-2006 | Andrew Titterell | Sale Sharks | England | 10-Jan-1981 | 25(124) |
| 14-May-2006 | Tom Voyce | London Wasps | England | 05-Jan-1981 | 25(129) |
| 14-May-2006 | Eion Redden | London Wasps | Ireland | 20-Nov-1980 | 25(175) |
| 14-May-2006 | Charlie Hodgson | Sale Sharks | England | 12-Nov-1980 | 25(183) |
| 14-May-2006 | Richard Skuse | London Irish | England | 11-Sep-1980 | 25(245) |
| 14-May-2006 | Chris Jones | Sale Sharks | England | 24-Aug-1980 | 25(263) |
| 14-May-2006 | Joe Ward | London Wasps | England | 03-Jun-1980 | 25(345) |
| 14-May-2006 | Jeremy Staunton | London Wasps | Ireland | 07-May-1980 | 26(7) |
| 14-May-2006 | Andy Goode | Leicester Tigers | England | 03-Apr-1980 | 26(41) |
| 14-May-2006 | Riki Flutey | London Irish | England | 10-Feb-1980 | 26(94) |
| 14-May-2006 | Declan Danagher | London Irish | England | 11-Jan-1980 | 26(124) |
| 14-May-2006 | Mark Cueto | Sale Sharks | England | 26-Dec-1979 | 26(140) |
| 14-May-2006 | Paul Sackey | London Wasps | England | 08-Nov-1979 | 26(188) |
| 14-May-2006 | Richard Birkett | London Wasps | England | 01-Oct-1979 | 26(226) |
| 14-May-2006 | Kieran Roche | London Irish | England | 03-May-1979 | 27(11) |
| 14-May-2006 | Tim Payne | London Wasps | England | 29-Apr-1979 | 27(15) |
| 14-May-2006 | Bob Casey | London Irish | Ireland | 18-Jul-1978 | 27(300) |
| 14-May-2006 | Elvis Seveali'i | Sale Sharks | Samoa | 20-Jun-1978 | 27(328) |
| 14-May-2006 | Dominic Feau'nati | London Irish | Samoa | 14-Jun-1978 | 27(334) |
| 14-May-2006 | Lewis Moody | Leicester Tigers | England | 12-Jun-1978 | 27(336) |
| 14-May-2006 | Stuart Abbott | London Wasps | England | 03-Jun-1978 | 27(345) |
| 14-May-2006 | Geordan Murphy | Leicester Tigers | Ireland | 19-Apr-1978 | 28(25) |
| 14-May-2006 | Jason White | Sale Sharks | Scotland | 17-Apr-1978 | 28(27) |
| 14-May-2006 | Leo Cullen | Leicester Tigers | Ireland | 09-Jan-1978 | 28(125) |
| 14-May-2006 | Sebastian Chabal | Sale Sharks | France | 08-Dec-1977 | 28(157) |
| 14-May-2006 | Peter Bracken | London Wasps | Ireland | 01-Dec-1977 | 28(164) |
| 14-May-2006 | Lionel Faure | Sale Sharks | France | 26-Nov-1977 | 28(169) |
| 14-May-2006 | Mark van Gisbergen | London Wasps | England | 30-Jun-1977 | 28(318) |
| 14-May-2006 | Joe Worsley | London Wasps | England | 14-Jun-1977 | 28(334) |
| 14-May-2006 | Sililo Martens | Sale Sharks | Tonga | 27-Apr-1977 | 29(17) |
| 14-May-2006 | Josh Lewsey | London Wasps | England | 30-Nov-1976 | 29(165) |
| 14-May-2006 | George Chuter | Leicester Tigers | England | 09-Jul-1976 | 29(309) |
| 14-May-2006 | Robbie Russell | London Irish | Scotland | 01-May-1976 | 30(13) |
| 14-May-2006 | Ben Kay | Leicester Tigers | England | 14-Dec-1975 | 30(152) |
| 14-May-2006 | Oriol Ripol | Sale Sharks | Spain | 06-Sep-1975 | 30(251) |
| 14-May-2006 | Darryl Gibson | Leicester Tigers | New Zealand | 02-Mar-1975 | 31(73) |
| 14-May-2006 | Ignacio Fernandez Lobbe | Sale Sharks | Argentina | 20-Nov-1974 | 31(175) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).