| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 05-May-2001 |  Ollie Smith | Leicester Tigers | England | 14-Aug-1982 | 18(265) |
| 29-Apr-2001 |  James Simpson-Daniel | Gloucester | England | 30-May-1982 | 18(335) |
| 28-Apr-2001 |  Mark Soden | Northampton Saints | England | 10-Apr-1981 | 20(18) |
| 28-Apr-2001 |  Andrew Lloyd | Bath | England | 09-Apr-1981 | 20(19) |
| 29-Apr-2001 | John Goodridge | Gloucester | England | 26-Feb-1981 | 20(62) |
| 28-Apr-2001 | Tom Voyce | Bath | England | 05-Jan-1981 | 20(113) |
| 13-May-2001 | Sam Cox | Bath | England | 11-Dec-1980 | 20(153) |
| 05-May-2001 | Louis Deacon | Leicester Tigers | England | 07-Oct-1980 | 20(210) |
| 28-Apr-2001 |  Michael Stephenson | Newcastle Falcons | England | 20-Sep-1980 | 20(220) |
| 28-Apr-2001 |  Luke Harbut | Saracens | England | 18-Apr-1980 | 21(10) |
| 28-Apr-2001 | Andy Goode | Leicester Tigers | England | 03-Apr-1980 | 21(25) |
| 28-Apr-2001 |  Robert Hoadley | London Irish | Ireland | 28-Mar-1980 | 21(31) |
| 28-Apr-2001 | David Flatman | Saracens | England | 21-Jan-1980 | 21(98) |
| 28-Apr-2001 | Declan Danagher | London Irish | England | 11-Jan-1980 | 21(108) |
| 28-Apr-2001 | Adam Balding | Leicester Tigers | England | 07-Dec-1979 | 21(143) |
| 28-Apr-2001 | Paul Sackey | London Irish | England | 08-Nov-1979 | 21(172) |
| 28-Apr-2001 | Steve Borthwick | Bath | England | 12-Oct-1979 | 21(199) |
| 29-Apr-2001 |  Richard Birkett | London Wasps | England | 01-Oct-1979 | 21(211) |
| 28-Apr-2001 | Kieran Campbell | London Irish | Ireland | 06-Jul-1979 | 21(297) |
| 05-May-2001 | Peter Short | Leicester Tigers | England | 20-Jun-1979 | 21(320) |
| 28-Apr-2001 | Jamie Noon | Newcastle Falcons | England | 09-May-1979 | 21(355) |
| 28-Apr-2001 | Gareth Cooper | Bath | Wales | 07-May-1979 | 21(357) |
| 28-Apr-2001 | Iain Balshaw | Bath | England | 18-Apr-1979 | 22(11) |
| 28-Apr-2001 | Epeli Taione | Newcastle Falcons | Tonga | 02-Mar-1979 | 22(58) |
| 28-Apr-2001 | Tom May | Newcastle Falcons | England | 05-Feb-1979 | 22(83) |
| 28-Apr-2001 | Micky Ward | Newcastle Falcons | England | 09-Jan-1979 | 22(110) |
| 28-Apr-2001 | Shaun Berne | Bath | Australia | 08-Jan-1979 | 22(111) |
| 28-Apr-2001 | Ben Johnston | Saracens | England | 08-Nov-1978 | 22(172) |
| 28-Apr-2001 | Ben Cohen | Northampton Saints | England | 14-Sep-1978 | 22(227) |
| 28-Apr-2001 | Anthony Roques | Saracens | England | 07-Sep-1978 | 22(234) |
| 28-Apr-2001 | Steve Thompson | Northampton Saints | England | 15-Jul-1978 | 22(288) |
| 28-Apr-2001 | Lewis Moody | Leicester Tigers | England | 12-Jun-1978 | 22(321) |
| 28-Apr-2001 | David Walder | Newcastle Falcons | England | 07-May-1978 | 22(357) |
| 29-Apr-2001 | Andrew Hazell | Gloucester | England | 25-Apr-1978 | 23(4) |
| 28-Apr-2001 | Geordan Murphy | Leicester Tigers | Ireland | 19-Apr-1978 | 23(9) |
| 28-Apr-2001 | Brett Sparg | Saracens | Namibia | 21-Mar-1978 | 23(38) |
| 28-Apr-2001 | Andrew Newman | Northampton Saints | Wales | 07-Jan-1978 | 23(111) |
| 28-Apr-2001 | Geoff Appleford | London Irish | England | 26-Sep-1977 | 23(214) |
| 28-Apr-2001 | Leon Lloyd | Leicester Tigers | England | 22-Sep-1977 | 23(218) |
| 28-Apr-2001 | Andy Long | Bath | England | 02-Sep-1977 | 23(238) |
| 28-Apr-2001 | Liam Botham | Newcastle Falcons | England | 26-Aug-1977 | 23(245) |
| 28-Apr-2001 | Ricky Nebbett | Leicester Tigers | England | 16-Aug-1977 | 23(255) |
| 29-Apr-2001 | Paul Sampson | London Wasps | England | 12-Jul-1977 | 23(291) |
| 29-Apr-2001 | Joe Worsley | London Wasps | England | 14-Jun-1977 | 23(319) |
| 29-Apr-2001 | Martyn Wood | London Wasps | England | 25-Apr-1977 | 24(4) |
| 28-Apr-2001 | Nmandi Ezulike | London Irish | * | 16-Feb-1977 | 24(71) |
| 28-Apr-2001 | Matt Perry | Bath | England | 27-Jan-1977 | 24(91) |
| 29-Apr-2001 | Josh Lewsey | London Wasps | England | 30-Nov-1976 | 24(150) |
| 28-Apr-2001 | Kelvin Todd | Northampton Saints | England | 23-Sep-1976 | 24(217) |
| 05-May-2001 | Steve Booth | Leicester Tigers | England | 18-Sep-1976 | 24(229) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).