| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 07-May-2005 |  Tom Rees | London Wasps | England | 11-Sep-1984 | 20(238) |
| 07-May-2005 |  Magnus Lund | Sale Sharks | England | 25-Jun-1983 | 21(317) |
| 14-May-2005 |  Ollie Smith | Leicester Tigers | England | 14-Aug-1982 | 22(274) |
| 14-May-2005 | Harry Ellis | Leicester Tigers | England | 17-May-1982 | 22(363) |
| 14-May-2005 | John Hart | London Wasps | England | 20-Mar-1982 | 23(55) |
| 14-May-2005 | Sam Vesty | Leicester Tigers | England | 26-Nov-1981 | 23(169) |
| 07-May-2005 | Andrew Titterell | Sale Sharks | England | 10-Jan-1981 | 24(117) |
| 07-May-2005 | Tom Voyce | London Wasps | England | 05-Jan-1981 | 24(122) |
| 07-May-2005 | Charlie Hodgson | Sale Sharks | England | 12-Nov-1980 | 24(176) |
| 14-May-2005 | Louis Deacon | Leicester Tigers | England | 07-Oct-1980 | 24(219) |
| 07-May-2005 | Chris Jones | Sale Sharks | England | 24-Aug-1980 | 24(256) |
| 14-May-2005 | Andy Goode | Leicester Tigers | England | 03-Apr-1980 | 25(41) |
| 07-May-2005 | Ayoola Erinle | London Wasps | England | 20-Feb-1980 | 25(77) |
| 07-May-2005 | Mark Cueto | Sale Sharks | England | 26-Dec-1979 | 25(133) |
| 07-May-2005 | Paul Sackey | London Wasps | England | 08-Nov-1979 | 25(181) |
| 07-May-2005 | Richard Birkett | London Wasps | England | 01-Oct-1979 | 25(219) |
| 07-May-2005 | Ben Coutts | Sale Sharks | Australia | 15-Sep-1979 | 25(235) |
| 07-May-2005 | Steve Hanley | Sale Sharks | England | 11-Jun-1979 | 25(331) |
| 07-May-2005 | Tim Payne | London Wasps | England | 29-Apr-1979 | 26(9) |
| 07-May-2005 | Dean Schofield | Sale Sharks | England | 19-Jan-1979 | 26(109) |
| 07-May-2005 | Jason White | Sale Sharks | Scotland | 17-Apr-1978 | 27(20) |
| 14-May-2005 | Geordan Murphy | Leicester Tigers | Ireland | 19-Apr-1978 | 27(25) |
| 07-May-2005 | Sebastian Chabal | Sale Sharks | France | 08-Dec-1977 | 27(150) |
| 14-May-2005 | Leon Lloyd | Leicester Tigers | England | 22-Sep-1977 | 27(234) |
| 07-May-2005 | Mark van Gisbergen | London Wasps | England | 30-Jun-1977 | 27(311) |
| 07-May-2005 | Joe Worsley | London Wasps | England | 14-Jun-1977 | 27(327) |
| 07-May-2005 | Josh Lewsey | London Wasps | England | 30-Nov-1976 | 28(158) |
| 14-May-2005 | George Chuter | Leicester Tigers | England | 09-Jul-1976 | 28(309) |
| 14-May-2005 | Ben Kay | Leicester Tigers | England | 14-Dec-1975 | 29(152) |
| 07-May-2005 | Phil Greening | London Wasps | England | 03-Oct-1975 | 29(217) |
| 07-May-2005 | Barry Stewart | Sale Sharks | Scotland | 03-Jun-1975 | 29(339) |
| 14-May-2005 | Darryl Gibson | Leicester Tigers | New Zealand | 02-Mar-1975 | 30(74) |
| 07-May-2005 | Alex King | London Wasps | England | 17-Jan-1975 | 30(111) |
| 14-May-2005 | Darren Morris | Leicester Tigers | Wales | 24-Sep-1974 | 30(233) |
| 07-May-2005 | Jason Robinson | Sale Sharks | England | 30-Jul-1974 | 30(282) |
| 07-May-2005 | Will Green | London Wasps | England | 25-Oct-1973 | 31(194) |
| 14-May-2005 | Martin Corry | Leicester Tigers | England | 12-Oct-1973 | 31(215) |
| 07-May-2005 | Simon Shaw | London Wasps | England | 01-Sep-1973 | 31(249) |
| 14-May-2005 | Julian White | Leicester Tigers | England | 14-May-1973 | 32(0) |
| 07-May-2005 | Jos Baxendell | Sale Sharks | England | 03-Dec-1972 | 32(155) |
| 07-May-2005 | Matt Dawson | London Wasps | England | 31-Oct-1972 | 32(188) |
| 07-May-2005 | Lawrence Dallaglio | London Wasps | England | 10-Aug-1972 | 32(270) |
| 07-May-2005 | Bryan Redpath | Sale Sharks | Scotland | 21-Jul-1971 | 33(291) |
| 07-May-2005 | Robert Todd | Sale Sharks | * | 19-Apr-1971 | 34(19) |
| 14-May-2005 | Martin Johnson | Leicester Tigers | England | 09-Mar-1970 | 35(67) |
| 14-May-2005 | Neil Back | Leicester Tigers | England | 16-Jan-1969 | 36(118) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).